Enhanced bone regeneration using an insulin-loaded nano-hydroxyapatite/collagen/PLGA composite scaffold

Xing Wang,1,* Guilan Zhang,2,* Feng Qi,3 Yongfeng Cheng,1 Xuguang Lu,1 Lu Wang,1 Jing Zhao,1 Bin Zhao1 1Shanxi Medical University Stomatological Hospital, Taiyuan, 2Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, China; 3Department of Mechanical and Aerospace En...

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Autores principales:	Wang X, Zhang G, Qi F, Cheng Y, Lu X, Wang L, Zhao J, Zhao B
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Insulin Composite Scaffold Drug delivery system Bone tissue engineering Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4e21bbd5798e4bb5bed9185c98242279
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spelling	oai:doaj.org-article:4e21bbd5798e4bb5bed9185c982422792021-12-02T05:39:46ZEnhanced bone regeneration using an insulin-loaded nano-hydroxyapatite/collagen/PLGA composite scaffold1178-2013https://doaj.org/article/4e21bbd5798e4bb5bed9185c982422792017-12-01T00:00:00Zhttps://www.dovepress.com/enhanced-bone-regeneration-using-an-insulin-loaded-nano-hydroxyapatite-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xing Wang,1,* Guilan Zhang,2,* Feng Qi,3 Yongfeng Cheng,1 Xuguang Lu,1 Lu Wang,1 Jing Zhao,1 Bin Zhao1 1Shanxi Medical University Stomatological Hospital, Taiyuan, 2Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, China; 3Department of Mechanical and Aerospace Engineering, University of Missouri, Columbia, MO, USA *These authors contributed equally to this work Abstract: Insulin is widely considered as a classical hormone and drug in maintaining energy and glucose homeostasis. Recently, insulin has been increasingly recognized as an indispensable factor for osteogenesis and bone turnover, but its applications in bone regeneration have been restricted because of the short periods of activity and uncontrolled release. In this study, we incorporated insulin-loaded poly lactic-co-glycolic-acid (PLGA) nanospheres into nano-hydroxyapatite/collagen (nHAC) scaffolds and investigated the bioactivity of the composite scaffolds in vitro and in vivo. Bioactive insulin was successfully released from the nanospheres within the scaffold, and the release kinetics of insulin could be efficiently controlled by uniform-sized nanospheres. The physical characterizations of the composite scaffolds demonstrated that incorporation of nanospheres in nHAC scaffolds using this method did not significantly change the porosity, pore diameters, and compressive strengths of nHAC. In vitro, the insulin-loaded nHAC/PLGA composite scaffolds possessed favorable biological function for bone marrow mesenchymal stem cells adhesion and proliferation, as well as the differentiation into osteoblasts. In vivo, the optimized bone regenerative capability of this composite scaffold was confirmed in rabbit mandible critical size defects. These results demonstrated successful development of a functional insulin–PLGA–nHAC composite scaffold that enhances the bone regeneration capability of nHAC. Keywords: insulin, composite scaffold, drug delivery system, bone tissue engineering Wang XZhang GQi FCheng YLu XWang LZhao JZhao BDove Medical PressarticleInsulinComposite ScaffoldDrug delivery systemBone tissue engineeringMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 117-127 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Insulin Composite Scaffold Drug delivery system Bone tissue engineering Medicine (General) R5-920
spellingShingle	Insulin Composite Scaffold Drug delivery system Bone tissue engineering Medicine (General) R5-920 Wang X Zhang G Qi F Cheng Y Lu X Wang L Zhao J Zhao B Enhanced bone regeneration using an insulin-loaded nano-hydroxyapatite/collagen/PLGA composite scaffold
description	Xing Wang,1,* Guilan Zhang,2,* Feng Qi,3 Yongfeng Cheng,1 Xuguang Lu,1 Lu Wang,1 Jing Zhao,1 Bin Zhao1 1Shanxi Medical University Stomatological Hospital, Taiyuan, 2Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, China; 3Department of Mechanical and Aerospace Engineering, University of Missouri, Columbia, MO, USA *These authors contributed equally to this work Abstract: Insulin is widely considered as a classical hormone and drug in maintaining energy and glucose homeostasis. Recently, insulin has been increasingly recognized as an indispensable factor for osteogenesis and bone turnover, but its applications in bone regeneration have been restricted because of the short periods of activity and uncontrolled release. In this study, we incorporated insulin-loaded poly lactic-co-glycolic-acid (PLGA) nanospheres into nano-hydroxyapatite/collagen (nHAC) scaffolds and investigated the bioactivity of the composite scaffolds in vitro and in vivo. Bioactive insulin was successfully released from the nanospheres within the scaffold, and the release kinetics of insulin could be efficiently controlled by uniform-sized nanospheres. The physical characterizations of the composite scaffolds demonstrated that incorporation of nanospheres in nHAC scaffolds using this method did not significantly change the porosity, pore diameters, and compressive strengths of nHAC. In vitro, the insulin-loaded nHAC/PLGA composite scaffolds possessed favorable biological function for bone marrow mesenchymal stem cells adhesion and proliferation, as well as the differentiation into osteoblasts. In vivo, the optimized bone regenerative capability of this composite scaffold was confirmed in rabbit mandible critical size defects. These results demonstrated successful development of a functional insulin–PLGA–nHAC composite scaffold that enhances the bone regeneration capability of nHAC. Keywords: insulin, composite scaffold, drug delivery system, bone tissue engineering
format	article
author	Wang X Zhang G Qi F Cheng Y Lu X Wang L Zhao J Zhao B
author_facet	Wang X Zhang G Qi F Cheng Y Lu X Wang L Zhao J Zhao B
author_sort	Wang X
title	Enhanced bone regeneration using an insulin-loaded nano-hydroxyapatite/collagen/PLGA composite scaffold
title_short	Enhanced bone regeneration using an insulin-loaded nano-hydroxyapatite/collagen/PLGA composite scaffold
title_full	Enhanced bone regeneration using an insulin-loaded nano-hydroxyapatite/collagen/PLGA composite scaffold
title_fullStr	Enhanced bone regeneration using an insulin-loaded nano-hydroxyapatite/collagen/PLGA composite scaffold
title_full_unstemmed	Enhanced bone regeneration using an insulin-loaded nano-hydroxyapatite/collagen/PLGA composite scaffold
title_sort	enhanced bone regeneration using an insulin-loaded nano-hydroxyapatite/collagen/plga composite scaffold
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/4e21bbd5798e4bb5bed9185c98242279
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Enhanced bone regeneration using an insulin-loaded nano-hydroxyapatite/collagen/PLGA composite scaffold

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