Host and Parasite Transcriptomic Changes upon Successive <named-content content-type="genus-species">Plasmodium falciparum</named-content> Infections in Early Childhood

ABSTRACT Children are highly susceptible to clinical malaria, and in regions where malaria is endemic, their immune systems must face successive encounters with Plasmodium falciparum parasites before they develop immunity, first against severe disease and later against uncomplicated malaria. Underst...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Katie R. Bradwell, Drissa Coulibaly, Abdoulaye K. Koné, Matthew B. Laurens, Ahmadou Dembélé, Youssouf Tolo, Karim Traoré, Amadou Niangaly, Andrea A. Berry, Bourema Kouriba, Christopher V. Plowe, Ogobara K. Doumbo, Kirsten E. Lyke, Shannon Takala-Harrison, Mahamadou A. Thera, Mark A. Travassos, David Serre
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	malaria transcriptomics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4e3104401ead46c1a245fe5ab39d23c3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4e3104401ead46c1a245fe5ab39d23c3
record_format	dspace
spelling	oai:doaj.org-article:4e3104401ead46c1a245fe5ab39d23c32021-12-02T18:44:37ZHost and Parasite Transcriptomic Changes upon Successive <named-content content-type="genus-species">Plasmodium falciparum</named-content> Infections in Early Childhood10.1128/mSystems.00116-202379-5077https://doaj.org/article/4e3104401ead46c1a245fe5ab39d23c32020-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00116-20https://doaj.org/toc/2379-5077ABSTRACT Children are highly susceptible to clinical malaria, and in regions where malaria is endemic, their immune systems must face successive encounters with Plasmodium falciparum parasites before they develop immunity, first against severe disease and later against uncomplicated malaria. Understanding cellular and molecular interactions between host and parasites during an infection could provide insights into the processes underlying this gradual acquisition of immunity, as well as to how parasites adapt to infect hosts that are successively more malaria experienced. Here, we describe methods to analyze the host and parasite gene expression profiles generated simultaneously from blood samples collected from five consecutive symptomatic P. falciparum infections in three Malian children. We show that the data generated enable statistical assessment of the proportions of (i) each white blood cell subset and (ii) the parasite developmental stages, as well as investigations of host-parasite gene coexpression. We also use the sequences generated to analyze allelic variations in transcribed regions and determine the complexity of each infection. While limited by the modest sample size, our analyses suggest that host gene expression profiles primarily clustered by individual, while the parasite gene expression profiles seemed to differentiate early from late infections. Overall, this study provides a solid framework to examine the mechanisms underlying acquisition of immunity to malaria infections using whole-blood transcriptome sequencing (RNA-seq). IMPORTANCE We show that dual RNA-seq from patient blood samples allows characterization of host/parasite interactions during malaria infections and can provide a solid framework to study the acquisition of antimalarial immunity, as well as the adaptations of P. falciparum to malaria-experienced hosts.Katie R. BradwellDrissa CoulibalyAbdoulaye K. KonéMatthew B. LaurensAhmadou DembéléYoussouf ToloKarim TraoréAmadou NiangalyAndrea A. BerryBourema KouribaChristopher V. PloweOgobara K. DoumboKirsten E. LykeShannon Takala-HarrisonMahamadou A. TheraMark A. TravassosDavid SerreAmerican Society for MicrobiologyarticlemalariatranscriptomicsMicrobiologyQR1-502ENmSystems, Vol 5, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	malaria transcriptomics Microbiology QR1-502
spellingShingle	malaria transcriptomics Microbiology QR1-502 Katie R. Bradwell Drissa Coulibaly Abdoulaye K. Koné Matthew B. Laurens Ahmadou Dembélé Youssouf Tolo Karim Traoré Amadou Niangaly Andrea A. Berry Bourema Kouriba Christopher V. Plowe Ogobara K. Doumbo Kirsten E. Lyke Shannon Takala-Harrison Mahamadou A. Thera Mark A. Travassos David Serre Host and Parasite Transcriptomic Changes upon Successive <named-content content-type="genus-species">Plasmodium falciparum</named-content> Infections in Early Childhood
description	ABSTRACT Children are highly susceptible to clinical malaria, and in regions where malaria is endemic, their immune systems must face successive encounters with Plasmodium falciparum parasites before they develop immunity, first against severe disease and later against uncomplicated malaria. Understanding cellular and molecular interactions between host and parasites during an infection could provide insights into the processes underlying this gradual acquisition of immunity, as well as to how parasites adapt to infect hosts that are successively more malaria experienced. Here, we describe methods to analyze the host and parasite gene expression profiles generated simultaneously from blood samples collected from five consecutive symptomatic P. falciparum infections in three Malian children. We show that the data generated enable statistical assessment of the proportions of (i) each white blood cell subset and (ii) the parasite developmental stages, as well as investigations of host-parasite gene coexpression. We also use the sequences generated to analyze allelic variations in transcribed regions and determine the complexity of each infection. While limited by the modest sample size, our analyses suggest that host gene expression profiles primarily clustered by individual, while the parasite gene expression profiles seemed to differentiate early from late infections. Overall, this study provides a solid framework to examine the mechanisms underlying acquisition of immunity to malaria infections using whole-blood transcriptome sequencing (RNA-seq). IMPORTANCE We show that dual RNA-seq from patient blood samples allows characterization of host/parasite interactions during malaria infections and can provide a solid framework to study the acquisition of antimalarial immunity, as well as the adaptations of P. falciparum to malaria-experienced hosts.
format	article
author	Katie R. Bradwell Drissa Coulibaly Abdoulaye K. Koné Matthew B. Laurens Ahmadou Dembélé Youssouf Tolo Karim Traoré Amadou Niangaly Andrea A. Berry Bourema Kouriba Christopher V. Plowe Ogobara K. Doumbo Kirsten E. Lyke Shannon Takala-Harrison Mahamadou A. Thera Mark A. Travassos David Serre
author_facet	Katie R. Bradwell Drissa Coulibaly Abdoulaye K. Koné Matthew B. Laurens Ahmadou Dembélé Youssouf Tolo Karim Traoré Amadou Niangaly Andrea A. Berry Bourema Kouriba Christopher V. Plowe Ogobara K. Doumbo Kirsten E. Lyke Shannon Takala-Harrison Mahamadou A. Thera Mark A. Travassos David Serre
author_sort	Katie R. Bradwell
title	Host and Parasite Transcriptomic Changes upon Successive <named-content content-type="genus-species">Plasmodium falciparum</named-content> Infections in Early Childhood
title_short	Host and Parasite Transcriptomic Changes upon Successive <named-content content-type="genus-species">Plasmodium falciparum</named-content> Infections in Early Childhood
title_full	Host and Parasite Transcriptomic Changes upon Successive <named-content content-type="genus-species">Plasmodium falciparum</named-content> Infections in Early Childhood
title_fullStr	Host and Parasite Transcriptomic Changes upon Successive <named-content content-type="genus-species">Plasmodium falciparum</named-content> Infections in Early Childhood
title_full_unstemmed	Host and Parasite Transcriptomic Changes upon Successive <named-content content-type="genus-species">Plasmodium falciparum</named-content> Infections in Early Childhood
title_sort	host and parasite transcriptomic changes upon successive <named-content content-type="genus-species">plasmodium falciparum</named-content> infections in early childhood
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/4e3104401ead46c1a245fe5ab39d23c3
work_keys_str_mv	AT katierbradwell hostandparasitetranscriptomicchangesuponsuccessivenamedcontentcontenttypegenusspeciesplasmodiumfalciparumnamedcontentinfectionsinearlychildhood AT drissacoulibaly hostandparasitetranscriptomicchangesuponsuccessivenamedcontentcontenttypegenusspeciesplasmodiumfalciparumnamedcontentinfectionsinearlychildhood AT abdoulayekkone hostandparasitetranscriptomicchangesuponsuccessivenamedcontentcontenttypegenusspeciesplasmodiumfalciparumnamedcontentinfectionsinearlychildhood AT matthewblaurens hostandparasitetranscriptomicchangesuponsuccessivenamedcontentcontenttypegenusspeciesplasmodiumfalciparumnamedcontentinfectionsinearlychildhood AT ahmadoudembele hostandparasitetranscriptomicchangesuponsuccessivenamedcontentcontenttypegenusspeciesplasmodiumfalciparumnamedcontentinfectionsinearlychildhood AT youssouftolo hostandparasitetranscriptomicchangesuponsuccessivenamedcontentcontenttypegenusspeciesplasmodiumfalciparumnamedcontentinfectionsinearlychildhood AT karimtraore hostandparasitetranscriptomicchangesuponsuccessivenamedcontentcontenttypegenusspeciesplasmodiumfalciparumnamedcontentinfectionsinearlychildhood AT amadouniangaly hostandparasitetranscriptomicchangesuponsuccessivenamedcontentcontenttypegenusspeciesplasmodiumfalciparumnamedcontentinfectionsinearlychildhood AT andreaaberry hostandparasitetranscriptomicchangesuponsuccessivenamedcontentcontenttypegenusspeciesplasmodiumfalciparumnamedcontentinfectionsinearlychildhood AT bouremakouriba hostandparasitetranscriptomicchangesuponsuccessivenamedcontentcontenttypegenusspeciesplasmodiumfalciparumnamedcontentinfectionsinearlychildhood AT christophervplowe hostandparasitetranscriptomicchangesuponsuccessivenamedcontentcontenttypegenusspeciesplasmodiumfalciparumnamedcontentinfectionsinearlychildhood AT ogobarakdoumbo hostandparasitetranscriptomicchangesuponsuccessivenamedcontentcontenttypegenusspeciesplasmodiumfalciparumnamedcontentinfectionsinearlychildhood AT kirstenelyke hostandparasitetranscriptomicchangesuponsuccessivenamedcontentcontenttypegenusspeciesplasmodiumfalciparumnamedcontentinfectionsinearlychildhood AT shannontakalaharrison hostandparasitetranscriptomicchangesuponsuccessivenamedcontentcontenttypegenusspeciesplasmodiumfalciparumnamedcontentinfectionsinearlychildhood AT mahamadouathera hostandparasitetranscriptomicchangesuponsuccessivenamedcontentcontenttypegenusspeciesplasmodiumfalciparumnamedcontentinfectionsinearlychildhood AT markatravassos hostandparasitetranscriptomicchangesuponsuccessivenamedcontentcontenttypegenusspeciesplasmodiumfalciparumnamedcontentinfectionsinearlychildhood AT davidserre hostandparasitetranscriptomicchangesuponsuccessivenamedcontentcontenttypegenusspeciesplasmodiumfalciparumnamedcontentinfectionsinearlychildhood
_version_	1718377679430877184

Host and Parasite Transcriptomic Changes upon Successive <named-content content-type="genus-species">Plasmodium falciparum</named-content> Infections in Early Childhood

Ejemplares similares