Short-term treatment with eicosapentaenoic acid improves inflammation and affects colonic differentiation markers and microbiota in patients with ulcerative colitis
Abstract Patients with long-standing ulcerative colitis (UC) have an increased colorectal cancer (CRC) risk. In this pilot study we evaluated the effect of Eicosapentaenoic acid as free fatty acid (EPA-FFA) supplementation on mucosal disease activity, colonic differentiation markers and microbiota c...
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Nature Portfolio
2017
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oai:doaj.org-article:4e3561bd67f740f69cdf357ffa89ab3f2021-12-02T15:05:57ZShort-term treatment with eicosapentaenoic acid improves inflammation and affects colonic differentiation markers and microbiota in patients with ulcerative colitis10.1038/s41598-017-07992-12045-2322https://doaj.org/article/4e3561bd67f740f69cdf357ffa89ab3f2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07992-1https://doaj.org/toc/2045-2322Abstract Patients with long-standing ulcerative colitis (UC) have an increased colorectal cancer (CRC) risk. In this pilot study we evaluated the effect of Eicosapentaenoic acid as free fatty acid (EPA-FFA) supplementation on mucosal disease activity, colonic differentiation markers and microbiota composition in UC patients. Twenty long-standing UC patients in stable clinical remission and with fecal calprotectin (FC) > 150 µg/g were enrolled (T0) and supplemented with EPA-FFA 2 g/daily for 90 days (T3). Endoscopic and histologic disease activities were measured by Mayo and Geboes scores, respectively. HES1, KLF4, STAT3, IL-10 and SOCS3 levels were determined using western blotting and qRT-PCR, while phospho-STAT3 levels were assessed by western blotting. Goblet cells were stained by Alcian blue. Microbiota analyses were performed on both fecal and colonic samples. Nineteen patients completed the study; seventeen (89.5%) were compliant. EPA-FFA treatment reduced FC levels at T3. Patients with FC > 150 µg/g at T3 (n = 2) were assumed as non-responders. EPA-FFA improved endoscopic and histological inflammation and induced IL-10, SOCS3, HES1 and KLF4 in compliant and responder patients. Importantly, long-term UC-driven microbiota composition was partially redressed by EPA-FFA. In conclusion, EPA-FFA supplementation reduced mucosal inflammation, promoted goblet cells differentiation and modulated intestinal microbiota composition in long-standing UC patients.Anna ProssomaritiEleonora ScaioliGiulia PiazziChiara FazioMatteo BellanovaElena BiagiMarco CandelaPatrizia BrigidiClarissa ConsolandiTiziana BalbiPasquale ChiecoAlessandra MunariniMilena ParialiManuela MinguzziFranco BazzoliAndrea BelluzziLuigi RicciardielloNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) |
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Medicine R Science Q Anna Prossomariti Eleonora Scaioli Giulia Piazzi Chiara Fazio Matteo Bellanova Elena Biagi Marco Candela Patrizia Brigidi Clarissa Consolandi Tiziana Balbi Pasquale Chieco Alessandra Munarini Milena Pariali Manuela Minguzzi Franco Bazzoli Andrea Belluzzi Luigi Ricciardiello Short-term treatment with eicosapentaenoic acid improves inflammation and affects colonic differentiation markers and microbiota in patients with ulcerative colitis |
| description |
Abstract Patients with long-standing ulcerative colitis (UC) have an increased colorectal cancer (CRC) risk. In this pilot study we evaluated the effect of Eicosapentaenoic acid as free fatty acid (EPA-FFA) supplementation on mucosal disease activity, colonic differentiation markers and microbiota composition in UC patients. Twenty long-standing UC patients in stable clinical remission and with fecal calprotectin (FC) > 150 µg/g were enrolled (T0) and supplemented with EPA-FFA 2 g/daily for 90 days (T3). Endoscopic and histologic disease activities were measured by Mayo and Geboes scores, respectively. HES1, KLF4, STAT3, IL-10 and SOCS3 levels were determined using western blotting and qRT-PCR, while phospho-STAT3 levels were assessed by western blotting. Goblet cells were stained by Alcian blue. Microbiota analyses were performed on both fecal and colonic samples. Nineteen patients completed the study; seventeen (89.5%) were compliant. EPA-FFA treatment reduced FC levels at T3. Patients with FC > 150 µg/g at T3 (n = 2) were assumed as non-responders. EPA-FFA improved endoscopic and histological inflammation and induced IL-10, SOCS3, HES1 and KLF4 in compliant and responder patients. Importantly, long-term UC-driven microbiota composition was partially redressed by EPA-FFA. In conclusion, EPA-FFA supplementation reduced mucosal inflammation, promoted goblet cells differentiation and modulated intestinal microbiota composition in long-standing UC patients. |
| format |
article |
| author |
Anna Prossomariti Eleonora Scaioli Giulia Piazzi Chiara Fazio Matteo Bellanova Elena Biagi Marco Candela Patrizia Brigidi Clarissa Consolandi Tiziana Balbi Pasquale Chieco Alessandra Munarini Milena Pariali Manuela Minguzzi Franco Bazzoli Andrea Belluzzi Luigi Ricciardiello |
| author_facet |
Anna Prossomariti Eleonora Scaioli Giulia Piazzi Chiara Fazio Matteo Bellanova Elena Biagi Marco Candela Patrizia Brigidi Clarissa Consolandi Tiziana Balbi Pasquale Chieco Alessandra Munarini Milena Pariali Manuela Minguzzi Franco Bazzoli Andrea Belluzzi Luigi Ricciardiello |
| author_sort |
Anna Prossomariti |
| title |
Short-term treatment with eicosapentaenoic acid improves inflammation and affects colonic differentiation markers and microbiota in patients with ulcerative colitis |
| title_short |
Short-term treatment with eicosapentaenoic acid improves inflammation and affects colonic differentiation markers and microbiota in patients with ulcerative colitis |
| title_full |
Short-term treatment with eicosapentaenoic acid improves inflammation and affects colonic differentiation markers and microbiota in patients with ulcerative colitis |
| title_fullStr |
Short-term treatment with eicosapentaenoic acid improves inflammation and affects colonic differentiation markers and microbiota in patients with ulcerative colitis |
| title_full_unstemmed |
Short-term treatment with eicosapentaenoic acid improves inflammation and affects colonic differentiation markers and microbiota in patients with ulcerative colitis |
| title_sort |
short-term treatment with eicosapentaenoic acid improves inflammation and affects colonic differentiation markers and microbiota in patients with ulcerative colitis |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/4e3561bd67f740f69cdf357ffa89ab3f |
| work_keys_str_mv |
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