Identification of MBW Complex Components Implicated in the Biosynthesis of Flavonoids in Woodland Strawberry

Flavonoids belong to the family of polyphenolic secondary metabolites and contribute to fruit quality traits. It has been shown that MBW complexes (MYB-bHLH-WD40) regulate the flavonoids biosynthesis in different plants, but only a limited number of MBW complexes have been identified in strawberry s...

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Autores principales:	Pengbo Xu, Liang Wu, Minghao Cao, Chao Ma, Kun Xiao, Yanbang Li, Hongli Lian
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	woodland strawberry MBW complex flavonoid biosynthesis MYB bHLH Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/4e3d0e858c184cd192a53c1f089698d6
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spelling	oai:doaj.org-article:4e3d0e858c184cd192a53c1f089698d62021-11-08T06:50:13ZIdentification of MBW Complex Components Implicated in the Biosynthesis of Flavonoids in Woodland Strawberry1664-462X10.3389/fpls.2021.774943https://doaj.org/article/4e3d0e858c184cd192a53c1f089698d62021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.774943/fullhttps://doaj.org/toc/1664-462XFlavonoids belong to the family of polyphenolic secondary metabolites and contribute to fruit quality traits. It has been shown that MBW complexes (MYB-bHLH-WD40) regulate the flavonoids biosynthesis in different plants, but only a limited number of MBW complexes have been identified in strawberry species in general. In this study, we identified 112 R2R3-MYB proteins in woodland strawberry; 12 of them were found to have potential functions in regulating flavonoids biosynthesis by phylogenetic analysis. qRT-PCR assays showed that FvMYB3, FvMYB9, FvMYB11, FvMYB22, FvMYB64, and FvMYB105 mostly expressed at green stage of fruit development, aligned with proanthocyanidins accumulation; FvMYB10 and FvMYB41 showed higher expression levels at turning and ripe stages, aligned with anthocyanins accumulation. These results suggest that different MYBs might be involved in flavonoids biosynthesis at specific stages. Furthermore, FvMYB proteins were demonstrated to interact with FvbHLH proteins and induce expression from the promoters of CHS2 and DFR2 genes, which encode key enzymes in flavonoids biosynthesis. The co-expression of FvMYB and FvbHLH proteins in strawberry fruits also promoted the accumulation of proanthocyanidins. These findings confirmed and provided insights into the biofunction of MBW components in the regulation of flavonoid biosynthesis in woodland strawberry.Pengbo XuLiang WuMinghao CaoChao MaKun XiaoYanbang LiHongli LianFrontiers Media S.A.articlewoodland strawberryMBW complexflavonoid biosynthesisMYBbHLHPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	woodland strawberry MBW complex flavonoid biosynthesis MYB bHLH Plant culture SB1-1110
spellingShingle	woodland strawberry MBW complex flavonoid biosynthesis MYB bHLH Plant culture SB1-1110 Pengbo Xu Liang Wu Minghao Cao Chao Ma Kun Xiao Yanbang Li Hongli Lian Identification of MBW Complex Components Implicated in the Biosynthesis of Flavonoids in Woodland Strawberry
description	Flavonoids belong to the family of polyphenolic secondary metabolites and contribute to fruit quality traits. It has been shown that MBW complexes (MYB-bHLH-WD40) regulate the flavonoids biosynthesis in different plants, but only a limited number of MBW complexes have been identified in strawberry species in general. In this study, we identified 112 R2R3-MYB proteins in woodland strawberry; 12 of them were found to have potential functions in regulating flavonoids biosynthesis by phylogenetic analysis. qRT-PCR assays showed that FvMYB3, FvMYB9, FvMYB11, FvMYB22, FvMYB64, and FvMYB105 mostly expressed at green stage of fruit development, aligned with proanthocyanidins accumulation; FvMYB10 and FvMYB41 showed higher expression levels at turning and ripe stages, aligned with anthocyanins accumulation. These results suggest that different MYBs might be involved in flavonoids biosynthesis at specific stages. Furthermore, FvMYB proteins were demonstrated to interact with FvbHLH proteins and induce expression from the promoters of CHS2 and DFR2 genes, which encode key enzymes in flavonoids biosynthesis. The co-expression of FvMYB and FvbHLH proteins in strawberry fruits also promoted the accumulation of proanthocyanidins. These findings confirmed and provided insights into the biofunction of MBW components in the regulation of flavonoid biosynthesis in woodland strawberry.
format	article
author	Pengbo Xu Liang Wu Minghao Cao Chao Ma Kun Xiao Yanbang Li Hongli Lian
author_facet	Pengbo Xu Liang Wu Minghao Cao Chao Ma Kun Xiao Yanbang Li Hongli Lian
author_sort	Pengbo Xu
title	Identification of MBW Complex Components Implicated in the Biosynthesis of Flavonoids in Woodland Strawberry
title_short	Identification of MBW Complex Components Implicated in the Biosynthesis of Flavonoids in Woodland Strawberry
title_full	Identification of MBW Complex Components Implicated in the Biosynthesis of Flavonoids in Woodland Strawberry
title_fullStr	Identification of MBW Complex Components Implicated in the Biosynthesis of Flavonoids in Woodland Strawberry
title_full_unstemmed	Identification of MBW Complex Components Implicated in the Biosynthesis of Flavonoids in Woodland Strawberry
title_sort	identification of mbw complex components implicated in the biosynthesis of flavonoids in woodland strawberry
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/4e3d0e858c184cd192a53c1f089698d6
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_version_	1718442906410287104

Identification of MBW Complex Components Implicated in the Biosynthesis of Flavonoids in Woodland Strawberry

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