Micro-computed tomography with contrast enhancement: An excellent technique for soft tissue examination in humans.

Manual dissection and histologic examination are commonly used to investigate human structures, but there are limitations in the damage caused to delicate structures or the provision of limited information. Micro-computed tomography (microCT) enables a three-dimensional volume-rendered observation o...

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Autores principales:	Jehoon O, Hyun-Jin Kwon, Tae-Hyeon Cho, Seung Hoon Woo, Yun-Hee Rhee, Hun-Mu Yang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4e43fc6456d94462be130b605805ef8f
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spelling	oai:doaj.org-article:4e43fc6456d94462be130b605805ef8f2021-12-02T20:09:19ZMicro-computed tomography with contrast enhancement: An excellent technique for soft tissue examination in humans.1932-620310.1371/journal.pone.0254264https://doaj.org/article/4e43fc6456d94462be130b605805ef8f2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254264https://doaj.org/toc/1932-6203Manual dissection and histologic examination are commonly used to investigate human structures, but there are limitations in the damage caused to delicate structures or the provision of limited information. Micro-computed tomography (microCT) enables a three-dimensional volume-rendered observation of the sample without destruction and deformation, but it can only visualize hard tissues in general. Therefore, contrast-enhancing agents are needed to help in visualizing soft tissue. This study aimed to introduce microCT with phosphotungstic acid preparation (PTA-microCT) by applying the method to different types of human tissue. Specimens from human cadavers were used to examine the orbicularis retaining ligament (ORL), nasolabial fold (NLF), and the calcaneal tunnel of the sole. Using PTA-microCT, relevant information of human structures was identified. In the ORL study, tiny and delicate ligamentous fibers were visualized in detail with multidirectional continuity. In the NLF study, complex structural formation consisting of various types of soft tissue were investigated comprehensively. In the calcaneal tunnel study, the space surrounded by diverse features and its inner vulnerable structures were examined without damage. Consequently, we successfully applied the PTA-microCT technique to the analysis of specific human soft tissue structures that are challenging to analyze by conventional methods.Jehoon OHyun-Jin KwonTae-Hyeon ChoSeung Hoon WooYun-Hee RheeHun-Mu YangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254264 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jehoon O Hyun-Jin Kwon Tae-Hyeon Cho Seung Hoon Woo Yun-Hee Rhee Hun-Mu Yang Micro-computed tomography with contrast enhancement: An excellent technique for soft tissue examination in humans.
description	Manual dissection and histologic examination are commonly used to investigate human structures, but there are limitations in the damage caused to delicate structures or the provision of limited information. Micro-computed tomography (microCT) enables a three-dimensional volume-rendered observation of the sample without destruction and deformation, but it can only visualize hard tissues in general. Therefore, contrast-enhancing agents are needed to help in visualizing soft tissue. This study aimed to introduce microCT with phosphotungstic acid preparation (PTA-microCT) by applying the method to different types of human tissue. Specimens from human cadavers were used to examine the orbicularis retaining ligament (ORL), nasolabial fold (NLF), and the calcaneal tunnel of the sole. Using PTA-microCT, relevant information of human structures was identified. In the ORL study, tiny and delicate ligamentous fibers were visualized in detail with multidirectional continuity. In the NLF study, complex structural formation consisting of various types of soft tissue were investigated comprehensively. In the calcaneal tunnel study, the space surrounded by diverse features and its inner vulnerable structures were examined without damage. Consequently, we successfully applied the PTA-microCT technique to the analysis of specific human soft tissue structures that are challenging to analyze by conventional methods.
format	article
author	Jehoon O Hyun-Jin Kwon Tae-Hyeon Cho Seung Hoon Woo Yun-Hee Rhee Hun-Mu Yang
author_facet	Jehoon O Hyun-Jin Kwon Tae-Hyeon Cho Seung Hoon Woo Yun-Hee Rhee Hun-Mu Yang
author_sort	Jehoon O
title	Micro-computed tomography with contrast enhancement: An excellent technique for soft tissue examination in humans.
title_short	Micro-computed tomography with contrast enhancement: An excellent technique for soft tissue examination in humans.
title_full	Micro-computed tomography with contrast enhancement: An excellent technique for soft tissue examination in humans.
title_fullStr	Micro-computed tomography with contrast enhancement: An excellent technique for soft tissue examination in humans.
title_full_unstemmed	Micro-computed tomography with contrast enhancement: An excellent technique for soft tissue examination in humans.
title_sort	micro-computed tomography with contrast enhancement: an excellent technique for soft tissue examination in humans.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/4e43fc6456d94462be130b605805ef8f
work_keys_str_mv	AT jehoono microcomputedtomographywithcontrastenhancementanexcellenttechniqueforsofttissueexaminationinhumans AT hyunjinkwon microcomputedtomographywithcontrastenhancementanexcellenttechniqueforsofttissueexaminationinhumans AT taehyeoncho microcomputedtomographywithcontrastenhancementanexcellenttechniqueforsofttissueexaminationinhumans AT seunghoonwoo microcomputedtomographywithcontrastenhancementanexcellenttechniqueforsofttissueexaminationinhumans AT yunheerhee microcomputedtomographywithcontrastenhancementanexcellenttechniqueforsofttissueexaminationinhumans AT hunmuyang microcomputedtomographywithcontrastenhancementanexcellenttechniqueforsofttissueexaminationinhumans
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Micro-computed tomography with contrast enhancement: An excellent technique for soft tissue examination in humans.

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