Differences in the biological properties of mesenchymal stromal cells from traumatic temporomandibular joint fibrous and bony ankylosis: a comparative study

The aim of this study was to compare the functional characteristics of mesenchymal stromal cells (MSCs) from a sheep model of traumatic temporomandibular joint (TMJ) fibrous and bony ankylosis. A sheep model of bilateral TMJ trauma-induced fibrous ankylosis on one side and bony ankylosis on the cont...

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Autores principales:	Pei-Pei Zhang, Su-Xia Liang, Hua-Lun Wang, Kun Yang, Shao-Chen Nie, Tong-Mei Zhang, Yuan-Yuan Tian, Zhao-Yuan Xu, Wei Chen, Ying-Bin Yan
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	ovine animal model mesenchymal stromal cells surface markers temporomandibular joint ankylosis trauma Medicine (General) R5-920 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4e45fc746ec447dd96f3fa980b751720
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spelling	oai:doaj.org-article:4e45fc746ec447dd96f3fa980b7517202021-11-04T15:00:42ZDifferences in the biological properties of mesenchymal stromal cells from traumatic temporomandibular joint fibrous and bony ankylosis: a comparative study1976-83542151-248510.1080/19768354.2021.1978543https://doaj.org/article/4e45fc746ec447dd96f3fa980b7517202021-09-01T00:00:00Zhttp://dx.doi.org/10.1080/19768354.2021.1978543https://doaj.org/toc/1976-8354https://doaj.org/toc/2151-2485The aim of this study was to compare the functional characteristics of mesenchymal stromal cells (MSCs) from a sheep model of traumatic temporomandibular joint (TMJ) fibrous and bony ankylosis. A sheep model of bilateral TMJ trauma-induced fibrous ankylosis on one side and bony ankylosis on the contralateral side was used. MSCs from fibrous ankylosed callus (FA-MSCs) or bony ankylosed callus (BA-MSCs) at weeks 1, 2, 4, and 8 after surgery were isolated and cultured. MSCs derived from the bone marrow of the mandibular condyle (BM-MSCs) were used as controls. The MSCs from the different sources were characterized morphologically, phenotypically, and functionally. Adherence and trilineage differentiation potential were presented in the ovine MSCs. These cell populations highly positively expressed MSC-associated specific markers, namely CD29, CD44, and CD166, but lacked CD31 and CD45 expressions. The BA-MSCs had higher clonogenic and proliferative potentials than the FA-MSCs. The BA-MSCs also showed higher osteogenic and chondrogenic potentials, but lower adipogenic capacity than the FA-MSCs. In addition, the BA-MSCs demonstrated higher chondrogenic, but lower osteogenic capacity than the BM-MSCs. Our study suggests that inhibition of the osteogenic and chondrogenic differentiations of MSCs might be a promising strategy for preventing bony ankylosis in the future.Pei-Pei ZhangSu-Xia LiangHua-Lun WangKun YangShao-Chen NieTong-Mei ZhangYuan-Yuan TianZhao-Yuan XuWei ChenYing-Bin YanTaylor & Francis Grouparticleovine animal modelmesenchymal stromal cellssurface markerstemporomandibular joint ankylosistraumaMedicine (General)R5-920Biology (General)QH301-705.5ENAnimal Cells and Systems, Vol 25, Iss 5, Pp 296-311 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ovine animal model mesenchymal stromal cells surface markers temporomandibular joint ankylosis trauma Medicine (General) R5-920 Biology (General) QH301-705.5
spellingShingle	ovine animal model mesenchymal stromal cells surface markers temporomandibular joint ankylosis trauma Medicine (General) R5-920 Biology (General) QH301-705.5 Pei-Pei Zhang Su-Xia Liang Hua-Lun Wang Kun Yang Shao-Chen Nie Tong-Mei Zhang Yuan-Yuan Tian Zhao-Yuan Xu Wei Chen Ying-Bin Yan Differences in the biological properties of mesenchymal stromal cells from traumatic temporomandibular joint fibrous and bony ankylosis: a comparative study
description	The aim of this study was to compare the functional characteristics of mesenchymal stromal cells (MSCs) from a sheep model of traumatic temporomandibular joint (TMJ) fibrous and bony ankylosis. A sheep model of bilateral TMJ trauma-induced fibrous ankylosis on one side and bony ankylosis on the contralateral side was used. MSCs from fibrous ankylosed callus (FA-MSCs) or bony ankylosed callus (BA-MSCs) at weeks 1, 2, 4, and 8 after surgery were isolated and cultured. MSCs derived from the bone marrow of the mandibular condyle (BM-MSCs) were used as controls. The MSCs from the different sources were characterized morphologically, phenotypically, and functionally. Adherence and trilineage differentiation potential were presented in the ovine MSCs. These cell populations highly positively expressed MSC-associated specific markers, namely CD29, CD44, and CD166, but lacked CD31 and CD45 expressions. The BA-MSCs had higher clonogenic and proliferative potentials than the FA-MSCs. The BA-MSCs also showed higher osteogenic and chondrogenic potentials, but lower adipogenic capacity than the FA-MSCs. In addition, the BA-MSCs demonstrated higher chondrogenic, but lower osteogenic capacity than the BM-MSCs. Our study suggests that inhibition of the osteogenic and chondrogenic differentiations of MSCs might be a promising strategy for preventing bony ankylosis in the future.
format	article
author	Pei-Pei Zhang Su-Xia Liang Hua-Lun Wang Kun Yang Shao-Chen Nie Tong-Mei Zhang Yuan-Yuan Tian Zhao-Yuan Xu Wei Chen Ying-Bin Yan
author_facet	Pei-Pei Zhang Su-Xia Liang Hua-Lun Wang Kun Yang Shao-Chen Nie Tong-Mei Zhang Yuan-Yuan Tian Zhao-Yuan Xu Wei Chen Ying-Bin Yan
author_sort	Pei-Pei Zhang
title	Differences in the biological properties of mesenchymal stromal cells from traumatic temporomandibular joint fibrous and bony ankylosis: a comparative study
title_short	Differences in the biological properties of mesenchymal stromal cells from traumatic temporomandibular joint fibrous and bony ankylosis: a comparative study
title_full	Differences in the biological properties of mesenchymal stromal cells from traumatic temporomandibular joint fibrous and bony ankylosis: a comparative study
title_fullStr	Differences in the biological properties of mesenchymal stromal cells from traumatic temporomandibular joint fibrous and bony ankylosis: a comparative study
title_full_unstemmed	Differences in the biological properties of mesenchymal stromal cells from traumatic temporomandibular joint fibrous and bony ankylosis: a comparative study
title_sort	differences in the biological properties of mesenchymal stromal cells from traumatic temporomandibular joint fibrous and bony ankylosis: a comparative study
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/4e45fc746ec447dd96f3fa980b751720
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Differences in the biological properties of mesenchymal stromal cells from traumatic temporomandibular joint fibrous and bony ankylosis: a comparative study

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