Circular RNA: Biosynthesis in vitro

Circular RNA: Biosynthesis in vitro

Circular RNA (circRNA) is a unique type of noncoding RNA molecule. Compared with traditional linear RNA, circRNA is a covalently closed circle produced by a process called backsplicing. CircRNA is abundant in many cells and has rich functions in cells, such as acting as miRNA sponge, protein sponge,...

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Autores principales: Xinjie Chen, Yuan Lu
Formato: article
Lenguaje:EN
Publicado: Frontiers Media S.A. 2021
Materias:
circular RNA
RNA synthesis
in vitro transcription
enzymatic ligation
ligase
permuted intron-exon
Biotechnology
TP248.13-248.65
Acceso en línea:https://doaj.org/article/4e46917b6d5c417bbf33eab7c8a18e49
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spelling oai:doaj.org-article:4e46917b6d5c417bbf33eab7c8a18e492021-12-01T19:04:07ZCircular RNA: Biosynthesis in vitro2296-418510.3389/fbioe.2021.787881https://doaj.org/article/4e46917b6d5c417bbf33eab7c8a18e492021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.787881/fullhttps://doaj.org/toc/2296-4185Circular RNA (circRNA) is a unique type of noncoding RNA molecule. Compared with traditional linear RNA, circRNA is a covalently closed circle produced by a process called backsplicing. CircRNA is abundant in many cells and has rich functions in cells, such as acting as miRNA sponge, protein sponge, protein scaffold, and mRNA regulator. With the continuous development of circRNA study, circRNA has also played an important role in medical applications, including circRNA vaccines and gene therapy. In this review, we illustrate the synthesis of circRNAs in vitro. We focus on biological ligation methods, such as enzymatic ligation from the bacteriophage T4 and ribozyme method. In addition, we summarize the current challenges in the design, synthesis, application, and production of circRNAs, and propose possible solutions in the future. CircRNA is expected to play an essential role in basic research and medical applications.Xinjie ChenYuan LuFrontiers Media S.A.articlecircular RNARNA synthesisin vitro transcriptionenzymatic ligationligasepermuted intron-exonBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution DOAJ
collection DOAJ
language EN
topic circular RNA
RNA synthesis
in vitro transcription
enzymatic ligation
ligase
permuted intron-exon
Biotechnology
TP248.13-248.65
spellingShingle circular RNA
RNA synthesis
in vitro transcription
enzymatic ligation
ligase
permuted intron-exon
Biotechnology
TP248.13-248.65
Xinjie Chen
Yuan Lu
Circular RNA: Biosynthesis in vitro
description Circular RNA (circRNA) is a unique type of noncoding RNA molecule. Compared with traditional linear RNA, circRNA is a covalently closed circle produced by a process called backsplicing. CircRNA is abundant in many cells and has rich functions in cells, such as acting as miRNA sponge, protein sponge, protein scaffold, and mRNA regulator. With the continuous development of circRNA study, circRNA has also played an important role in medical applications, including circRNA vaccines and gene therapy. In this review, we illustrate the synthesis of circRNAs in vitro. We focus on biological ligation methods, such as enzymatic ligation from the bacteriophage T4 and ribozyme method. In addition, we summarize the current challenges in the design, synthesis, application, and production of circRNAs, and propose possible solutions in the future. CircRNA is expected to play an essential role in basic research and medical applications.
format article
author Xinjie Chen
Yuan Lu
author_facet Xinjie Chen
Yuan Lu
author_sort Xinjie Chen
title Circular RNA: Biosynthesis in vitro
title_short Circular RNA: Biosynthesis in vitro
title_full Circular RNA: Biosynthesis in vitro
title_fullStr Circular RNA: Biosynthesis in vitro
title_full_unstemmed Circular RNA: Biosynthesis in vitro
title_sort circular rna: biosynthesis in vitro
publisher Frontiers Media S.A.
publishDate 2021
url https://doaj.org/article/4e46917b6d5c417bbf33eab7c8a18e49
work_keys_str_mv AT xinjiechen circularrnabiosynthesisinvitro
AT yuanlu circularrnabiosynthesisinvitro
_version_ 1718404630645309440

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