Differences in mucosal gene expression in the colon of two inbred mouse strains after colonization with commensal gut bacteria.

The host genotype has been proposed to contribute to individually composed bacterial communities in the gut. To provide deeper insight into interactions between gut bacteria and host, we associated germ-free C3H and C57BL/10 mice with intestinal bacteria from a C57BL/10 donor mouse. Analysis of micr...

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Autores principales:	Frances Brodziak, Caroline Meharg, Michael Blaut, Gunnar Loh
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4e4802a38a3449e0aa215ad75ed26b55
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spelling	oai:doaj.org-article:4e4802a38a3449e0aa215ad75ed26b552021-11-18T09:00:23ZDifferences in mucosal gene expression in the colon of two inbred mouse strains after colonization with commensal gut bacteria.1932-620310.1371/journal.pone.0072317https://doaj.org/article/4e4802a38a3449e0aa215ad75ed26b552013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23951309/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The host genotype has been proposed to contribute to individually composed bacterial communities in the gut. To provide deeper insight into interactions between gut bacteria and host, we associated germ-free C3H and C57BL/10 mice with intestinal bacteria from a C57BL/10 donor mouse. Analysis of microbiota similarity between the animals with denaturing gradient gel electrophoresis revealed the development of a mouse strain-specific microbiota. Microarray-based gene expression analysis in the colonic mucosa identified 202 genes whose expression differed significantly by a factor of more than 2. Application of bioinformatics tools demonstrated that functional terms including signaling/secretion, lipid degradation/catabolism, guanine nucleotide/guanylate binding and immune response were significantly enriched in differentially expressed genes. We had a closer look at the 56 genes with expression differences of more than 4 and observed a higher expression in C57BL/10 mice of the genes coding for Tlr1 and Ang4 which are involved in the recognition and response to gut bacteria. A higher expression of Pla2g2a was detected in C3H mice. In addition, a number of interferon-inducible genes were higher expressed in C3H than in C57BL/10 mice including Gbp1, Mal, Oasl2, Ifi202b, Rtp4, Ly6g6c, Ifi27l2a, Usp18, Ifit1, Ifi44, and Ly6g indicating that interferons may play an essential role in microbiota regulation. However, genes coding for interferons, their receptors, factors involved in interferon expression regulation or signaling pathways were not differentially expressed between the two mouse strains. Taken together, our study confirms that the host genotype is involved in the establishment of host-specific bacterial communities in the gut. Based on expression differences after colonization with the same bacterial inoculum, we propose that Pla2g2a and interferon-dependent genes may contribute to this phenomenon.Frances BrodziakCaroline MehargMichael BlautGunnar LohPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e72317 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Frances Brodziak Caroline Meharg Michael Blaut Gunnar Loh Differences in mucosal gene expression in the colon of two inbred mouse strains after colonization with commensal gut bacteria.
description	The host genotype has been proposed to contribute to individually composed bacterial communities in the gut. To provide deeper insight into interactions between gut bacteria and host, we associated germ-free C3H and C57BL/10 mice with intestinal bacteria from a C57BL/10 donor mouse. Analysis of microbiota similarity between the animals with denaturing gradient gel electrophoresis revealed the development of a mouse strain-specific microbiota. Microarray-based gene expression analysis in the colonic mucosa identified 202 genes whose expression differed significantly by a factor of more than 2. Application of bioinformatics tools demonstrated that functional terms including signaling/secretion, lipid degradation/catabolism, guanine nucleotide/guanylate binding and immune response were significantly enriched in differentially expressed genes. We had a closer look at the 56 genes with expression differences of more than 4 and observed a higher expression in C57BL/10 mice of the genes coding for Tlr1 and Ang4 which are involved in the recognition and response to gut bacteria. A higher expression of Pla2g2a was detected in C3H mice. In addition, a number of interferon-inducible genes were higher expressed in C3H than in C57BL/10 mice including Gbp1, Mal, Oasl2, Ifi202b, Rtp4, Ly6g6c, Ifi27l2a, Usp18, Ifit1, Ifi44, and Ly6g indicating that interferons may play an essential role in microbiota regulation. However, genes coding for interferons, their receptors, factors involved in interferon expression regulation or signaling pathways were not differentially expressed between the two mouse strains. Taken together, our study confirms that the host genotype is involved in the establishment of host-specific bacterial communities in the gut. Based on expression differences after colonization with the same bacterial inoculum, we propose that Pla2g2a and interferon-dependent genes may contribute to this phenomenon.
format	article
author	Frances Brodziak Caroline Meharg Michael Blaut Gunnar Loh
author_facet	Frances Brodziak Caroline Meharg Michael Blaut Gunnar Loh
author_sort	Frances Brodziak
title	Differences in mucosal gene expression in the colon of two inbred mouse strains after colonization with commensal gut bacteria.
title_short	Differences in mucosal gene expression in the colon of two inbred mouse strains after colonization with commensal gut bacteria.
title_full	Differences in mucosal gene expression in the colon of two inbred mouse strains after colonization with commensal gut bacteria.
title_fullStr	Differences in mucosal gene expression in the colon of two inbred mouse strains after colonization with commensal gut bacteria.
title_full_unstemmed	Differences in mucosal gene expression in the colon of two inbred mouse strains after colonization with commensal gut bacteria.
title_sort	differences in mucosal gene expression in the colon of two inbred mouse strains after colonization with commensal gut bacteria.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/4e4802a38a3449e0aa215ad75ed26b55
work_keys_str_mv	AT francesbrodziak differencesinmucosalgeneexpressioninthecolonoftwoinbredmousestrainsaftercolonizationwithcommensalgutbacteria AT carolinemeharg differencesinmucosalgeneexpressioninthecolonoftwoinbredmousestrainsaftercolonizationwithcommensalgutbacteria AT michaelblaut differencesinmucosalgeneexpressioninthecolonoftwoinbredmousestrainsaftercolonizationwithcommensalgutbacteria AT gunnarloh differencesinmucosalgeneexpressioninthecolonoftwoinbredmousestrainsaftercolonizationwithcommensalgutbacteria
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Differences in mucosal gene expression in the colon of two inbred mouse strains after colonization with commensal gut bacteria.

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