An activity-dependent proximity ligation platform for spatially resolved quantification of active enzymes in single cells

The interrogation of enzyme activity involves the ensemble averaging of many cells, loss of spatial relationships and is often biased to abundant proteins. Here the authors develop activity-dependent proximity ligation to quantify enzyme activity at the cellular and sub-cellular level in relevant bi...

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Autores principales:	Gang Li, Jeffrey E. Montgomery, Mark A. Eckert, Jae Won Chang, Samantha M. Tienda, Ernst Lengyel, Raymond E. Moellering
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4e4b7627eb0e4daf97d1194c3f8abb64
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spelling	oai:doaj.org-article:4e4b7627eb0e4daf97d1194c3f8abb642021-12-02T17:06:10ZAn activity-dependent proximity ligation platform for spatially resolved quantification of active enzymes in single cells10.1038/s41467-017-01854-02041-1723https://doaj.org/article/4e4b7627eb0e4daf97d1194c3f8abb642017-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-01854-0https://doaj.org/toc/2041-1723The interrogation of enzyme activity involves the ensemble averaging of many cells, loss of spatial relationships and is often biased to abundant proteins. Here the authors develop activity-dependent proximity ligation to quantify enzyme activity at the cellular and sub-cellular level in relevant biological contexts.Gang LiJeffrey E. MontgomeryMark A. EckertJae Won ChangSamantha M. TiendaErnst LengyelRaymond E. MoelleringNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Gang Li Jeffrey E. Montgomery Mark A. Eckert Jae Won Chang Samantha M. Tienda Ernst Lengyel Raymond E. Moellering An activity-dependent proximity ligation platform for spatially resolved quantification of active enzymes in single cells
description	The interrogation of enzyme activity involves the ensemble averaging of many cells, loss of spatial relationships and is often biased to abundant proteins. Here the authors develop activity-dependent proximity ligation to quantify enzyme activity at the cellular and sub-cellular level in relevant biological contexts.
format	article
author	Gang Li Jeffrey E. Montgomery Mark A. Eckert Jae Won Chang Samantha M. Tienda Ernst Lengyel Raymond E. Moellering
author_facet	Gang Li Jeffrey E. Montgomery Mark A. Eckert Jae Won Chang Samantha M. Tienda Ernst Lengyel Raymond E. Moellering
author_sort	Gang Li
title	An activity-dependent proximity ligation platform for spatially resolved quantification of active enzymes in single cells
title_short	An activity-dependent proximity ligation platform for spatially resolved quantification of active enzymes in single cells
title_full	An activity-dependent proximity ligation platform for spatially resolved quantification of active enzymes in single cells
title_fullStr	An activity-dependent proximity ligation platform for spatially resolved quantification of active enzymes in single cells
title_full_unstemmed	An activity-dependent proximity ligation platform for spatially resolved quantification of active enzymes in single cells
title_sort	activity-dependent proximity ligation platform for spatially resolved quantification of active enzymes in single cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4e4b7627eb0e4daf97d1194c3f8abb64
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An activity-dependent proximity ligation platform for spatially resolved quantification of active enzymes in single cells

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