Punica granatum L. extract contributes to phytopathogens control and enhances Eruca vesicaria (L.) Cav. germination in vitro and in vivo

The study aimed to investigate antimicrobial activity of the hydroalcoholic crude extract from the fruit peel of Punica granatum (Pp) and punicalagin compound (Pg) on phytopathogenic bacterial isolates and its potential use as a sustainable alternative in treatment of vegetable seeds. The antimicro...

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Autores principales:	Suzanna de Sousa Silva, Patrícia Costa dos Santos Alves, Denise Fernandes Coutinho, Tássio Rômulo Silva Araújo Luz, Guilherme Martins Gomes Fontoura, Andresa Aparecida Berretta, Marcia Cristina Gonçalves Maciel
Formato:	article
Lenguaje:	EN
Publicado:	Firenze University Press 2021
Materias:	antimicrobial botanical pesticides food security plant diseases pomegranate peel punicalagin Biology (General) QH301-705.5 Botany QK1-989
Acceso en línea:	https://doaj.org/article/4e4cd954a452477cbc0d138e6320470c
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spelling	oai:doaj.org-article:4e4cd954a452477cbc0d138e6320470c2021-11-08T13:15:49ZPunica granatum L. extract contributes to phytopathogens control and enhances Eruca vesicaria (L.) Cav. germination in vitro and in vivo10.36253/ahsc-107060394-61691592-1573https://doaj.org/article/4e4cd954a452477cbc0d138e6320470c2021-05-01T00:00:00Zhttps://oaj.fupress.net/index.php/ahs/article/view/10706https://doaj.org/toc/0394-6169https://doaj.org/toc/1592-1573 The study aimed to investigate antimicrobial activity of the hydroalcoholic crude extract from the fruit peel of Punica granatum (Pp) and punicalagin compound (Pg) on phytopathogenic bacterial isolates and its potential use as a sustainable alternative in treatment of vegetable seeds. The antimicrobial activity in vitro was tested by agar well diffusion assay and through viability tests in liquid medium. In vivo treatment with Pp was tested on Eruca vesicaria seeds infected with Xanthomonas campestris pv. campestris. Pp induced the formation of large inhibition zones to the growth of the tested pathogens (35.33 mm – 6.66 mm), with dose-dependent effect. Viability tests confirmed the antimicrobial activity of the Pp on X. campestris pv. campestris and P. carotovorum subsp. carotovorum with minimum inhibitory concentration (MIC) of 125 μg/mL. Punicalagin compound presented MIC of the 31.25 μg/mL. The seed treatment with Pp indicated control of pathogen-induced symptoms in seedlings of the E. vesicaria and positive effect in seed germination, emergence and in stomatal functionality. The results indicate strong potential of the extract from the fruit peel of P. granatum and Punicalagin for formulating botanical pesticides for plant disease control. Suzanna de Sousa SilvaPatrícia Costa dos Santos AlvesDenise Fernandes CoutinhoTássio Rômulo Silva Araújo LuzGuilherme Martins Gomes FontouraAndresa Aparecida BerrettaMarcia Cristina Gonçalves MacielFirenze University Pressarticleantimicrobialbotanical pesticidesfood securityplant diseasespomegranate peelpunicalaginBiology (General)QH301-705.5BotanyQK1-989ENAdvances in Horticultural Science, Vol 35, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antimicrobial botanical pesticides food security plant diseases pomegranate peel punicalagin Biology (General) QH301-705.5 Botany QK1-989
spellingShingle	antimicrobial botanical pesticides food security plant diseases pomegranate peel punicalagin Biology (General) QH301-705.5 Botany QK1-989 Suzanna de Sousa Silva Patrícia Costa dos Santos Alves Denise Fernandes Coutinho Tássio Rômulo Silva Araújo Luz Guilherme Martins Gomes Fontoura Andresa Aparecida Berretta Marcia Cristina Gonçalves Maciel Punica granatum L. extract contributes to phytopathogens control and enhances Eruca vesicaria (L.) Cav. germination in vitro and in vivo
description	The study aimed to investigate antimicrobial activity of the hydroalcoholic crude extract from the fruit peel of Punica granatum (Pp) and punicalagin compound (Pg) on phytopathogenic bacterial isolates and its potential use as a sustainable alternative in treatment of vegetable seeds. The antimicrobial activity in vitro was tested by agar well diffusion assay and through viability tests in liquid medium. In vivo treatment with Pp was tested on Eruca vesicaria seeds infected with Xanthomonas campestris pv. campestris. Pp induced the formation of large inhibition zones to the growth of the tested pathogens (35.33 mm – 6.66 mm), with dose-dependent effect. Viability tests confirmed the antimicrobial activity of the Pp on X. campestris pv. campestris and P. carotovorum subsp. carotovorum with minimum inhibitory concentration (MIC) of 125 μg/mL. Punicalagin compound presented MIC of the 31.25 μg/mL. The seed treatment with Pp indicated control of pathogen-induced symptoms in seedlings of the E. vesicaria and positive effect in seed germination, emergence and in stomatal functionality. The results indicate strong potential of the extract from the fruit peel of P. granatum and Punicalagin for formulating botanical pesticides for plant disease control.
format	article
author	Suzanna de Sousa Silva Patrícia Costa dos Santos Alves Denise Fernandes Coutinho Tássio Rômulo Silva Araújo Luz Guilherme Martins Gomes Fontoura Andresa Aparecida Berretta Marcia Cristina Gonçalves Maciel
author_facet	Suzanna de Sousa Silva Patrícia Costa dos Santos Alves Denise Fernandes Coutinho Tássio Rômulo Silva Araújo Luz Guilherme Martins Gomes Fontoura Andresa Aparecida Berretta Marcia Cristina Gonçalves Maciel
author_sort	Suzanna de Sousa Silva
title	Punica granatum L. extract contributes to phytopathogens control and enhances Eruca vesicaria (L.) Cav. germination in vitro and in vivo
title_short	Punica granatum L. extract contributes to phytopathogens control and enhances Eruca vesicaria (L.) Cav. germination in vitro and in vivo
title_full	Punica granatum L. extract contributes to phytopathogens control and enhances Eruca vesicaria (L.) Cav. germination in vitro and in vivo
title_fullStr	Punica granatum L. extract contributes to phytopathogens control and enhances Eruca vesicaria (L.) Cav. germination in vitro and in vivo
title_full_unstemmed	Punica granatum L. extract contributes to phytopathogens control and enhances Eruca vesicaria (L.) Cav. germination in vitro and in vivo
title_sort	punica granatum l. extract contributes to phytopathogens control and enhances eruca vesicaria (l.) cav. germination in vitro and in vivo
publisher	Firenze University Press
publishDate	2021
url	https://doaj.org/article/4e4cd954a452477cbc0d138e6320470c
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Punica granatum L. extract contributes to phytopathogens control and enhances Eruca vesicaria (L.) Cav. germination in vitro and in vivo

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