One-Pot Scalable Synthesis of rGO/AuNPs Nanocomposite and Its Application in Enzymatic Glucose Biosensor
A nanocomposite, rGO/AuNPs, was synthesized simultaneously using one-pot approach with gold nanoparticles (AuNPs) and reduced graphene oxide (rGO) within gelatin which used as a reducing and stabilizing agent. Then, the fabricated nanocomposites were characterized by UV-Vis, transmission electron mi...
Guardado en:
| Autor principal: | |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Taylor & Francis Group
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/4e50cda719aa484782f259a11aa09627 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| Sumario: | A nanocomposite, rGO/AuNPs, was synthesized simultaneously using one-pot approach with gold nanoparticles (AuNPs) and reduced graphene oxide (rGO) within gelatin which used as a reducing and stabilizing agent. Then, the fabricated nanocomposites were characterized by UV-Vis, transmission electron microscope (TEM) and field emission scanning electron microscope (FE-SEM). The optimal nanocomposite was determined using electrochemical approaches. After facile and eco-friendly synthesis of rGO/AuNPs nanocomposites, it was used to fabricate the bioanode of enzymatic glucose biosensors. After drop-casting the nanocomposites on a screen-printed electrode (SPE), the glucose oxidase (GOx) was immobilized on the pre-treated SPE through a protein cross-linking approach using glutaraldehyde (GA) as a crosslinking reagent and 2,5-dihydroxybenzaldehyde (DHB) as a mediator to improve the electrochemical performance. Then, electrochemical performance of enzyme immobilized nanocomposites was studied using the potentiostat. The results demonstrate that the enzymatic biosensor made of rGO/AuNPs nanocomposites showed enhanced the sensitivity of selectivity for detection of glucose. |
|---|