Isolation and characterization of a halophilic Modicisalibacter sp. strain Wilcox from produced water

Abstract We report the isolation a halophilic bacterium that degrades both aromatic and aliphatic hydrocarbons as the sole sources of carbon at high salinity from produced water. Phylogenetic analysis of 16S rRNA-gene sequences shows the isolate is a close relative of Modicisalibacter tunisiensis is...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	William S. Marsh, Brenden W. Heise, Mark J. Krzmarzick, Robert W. Murdoch, Babu Z. Fathepure
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4e59ee327fc447fa8455983ed4185ab8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4e59ee327fc447fa8455983ed4185ab8
record_format	dspace
spelling	oai:doaj.org-article:4e59ee327fc447fa8455983ed4185ab82021-12-02T16:36:12ZIsolation and characterization of a halophilic Modicisalibacter sp. strain Wilcox from produced water10.1038/s41598-021-86196-02045-2322https://doaj.org/article/4e59ee327fc447fa8455983ed4185ab82021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86196-0https://doaj.org/toc/2045-2322Abstract We report the isolation a halophilic bacterium that degrades both aromatic and aliphatic hydrocarbons as the sole sources of carbon at high salinity from produced water. Phylogenetic analysis of 16S rRNA-gene sequences shows the isolate is a close relative of Modicisalibacter tunisiensis isolated from an oil-field water in Tunisia. We designate our isolate as Modicisalibacter sp. strain Wilcox. Genome analysis of strain Wilcox revealed the presence of a repertoire of genes involved in the metabolism of aliphatic and aromatic hydrocarbons. Laboratory culture studies corroborated the predicted hydrocarbon degradation potential. The strain degraded benzene, toluene, ethylbenzene, and xylenes at salinities ranging from 0.016 to 4.0 M NaCl, with optimal degradation at 1 M NaCl. Also, the strain degraded phenol, benzoate, biphenyl and phenylacetate as the sole sources of carbon at 2.5 M NaCl. Among aliphatic compounds, the strain degraded n-decane and n-hexadecane as the sole sources of carbon at 2.5 M NaCl. Genome analysis also predicted the presence of many heavy metal resistance genes including genes for metal efflux pumps, transport proteins, and enzymatic detoxification. Overall, due to its ability to degrade many hydrocarbons and withstand high salt and heavy metals, strain Wilcox may prove useful for remediation of produced waters.William S. MarshBrenden W. HeiseMark J. KrzmarzickRobert W. MurdochBabu Z. FathepureNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q William S. Marsh Brenden W. Heise Mark J. Krzmarzick Robert W. Murdoch Babu Z. Fathepure Isolation and characterization of a halophilic Modicisalibacter sp. strain Wilcox from produced water
description	Abstract We report the isolation a halophilic bacterium that degrades both aromatic and aliphatic hydrocarbons as the sole sources of carbon at high salinity from produced water. Phylogenetic analysis of 16S rRNA-gene sequences shows the isolate is a close relative of Modicisalibacter tunisiensis isolated from an oil-field water in Tunisia. We designate our isolate as Modicisalibacter sp. strain Wilcox. Genome analysis of strain Wilcox revealed the presence of a repertoire of genes involved in the metabolism of aliphatic and aromatic hydrocarbons. Laboratory culture studies corroborated the predicted hydrocarbon degradation potential. The strain degraded benzene, toluene, ethylbenzene, and xylenes at salinities ranging from 0.016 to 4.0 M NaCl, with optimal degradation at 1 M NaCl. Also, the strain degraded phenol, benzoate, biphenyl and phenylacetate as the sole sources of carbon at 2.5 M NaCl. Among aliphatic compounds, the strain degraded n-decane and n-hexadecane as the sole sources of carbon at 2.5 M NaCl. Genome analysis also predicted the presence of many heavy metal resistance genes including genes for metal efflux pumps, transport proteins, and enzymatic detoxification. Overall, due to its ability to degrade many hydrocarbons and withstand high salt and heavy metals, strain Wilcox may prove useful for remediation of produced waters.
format	article
author	William S. Marsh Brenden W. Heise Mark J. Krzmarzick Robert W. Murdoch Babu Z. Fathepure
author_facet	William S. Marsh Brenden W. Heise Mark J. Krzmarzick Robert W. Murdoch Babu Z. Fathepure
author_sort	William S. Marsh
title	Isolation and characterization of a halophilic Modicisalibacter sp. strain Wilcox from produced water
title_short	Isolation and characterization of a halophilic Modicisalibacter sp. strain Wilcox from produced water
title_full	Isolation and characterization of a halophilic Modicisalibacter sp. strain Wilcox from produced water
title_fullStr	Isolation and characterization of a halophilic Modicisalibacter sp. strain Wilcox from produced water
title_full_unstemmed	Isolation and characterization of a halophilic Modicisalibacter sp. strain Wilcox from produced water
title_sort	isolation and characterization of a halophilic modicisalibacter sp. strain wilcox from produced water
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4e59ee327fc447fa8455983ed4185ab8
work_keys_str_mv	AT williamsmarsh isolationandcharacterizationofahalophilicmodicisalibacterspstrainwilcoxfromproducedwater AT brendenwheise isolationandcharacterizationofahalophilicmodicisalibacterspstrainwilcoxfromproducedwater AT markjkrzmarzick isolationandcharacterizationofahalophilicmodicisalibacterspstrainwilcoxfromproducedwater AT robertwmurdoch isolationandcharacterizationofahalophilicmodicisalibacterspstrainwilcoxfromproducedwater AT babuzfathepure isolationandcharacterizationofahalophilicmodicisalibacterspstrainwilcoxfromproducedwater
_version_	1718383632003891200

Isolation and characterization of a halophilic Modicisalibacter sp. strain Wilcox from produced water

Ejemplares similares