Hierarchical modelling of immunoglobulin coated bacteria in dogs with chronic enteropathy shows reduction in coating with disease remission but marked inter-individual and treatment-response variability.

Chronic enteropathies are a common problem in dogs, but many aspects of the pathogenesis remain unknown, making the therapeutic approach challenging in some cases. Environmental factors are intimately related to the development and perpetuation of gastrointestinal disease and the gut microbiome has...

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Autores principales:	Lina María Martínez-López, Alexis Perez-Gonzalez, Elizabeth Ann Washington, Andrew P Woodward, Alexandra Jazmin Roth-Schulze, Julien R S Dandrieux, Thurid Johnstone, Nathalee Prakash, Aaron Jex, Caroline Mansfield
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4e6362fba2be42debfa0b962e172d096
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spelling	oai:doaj.org-article:4e6362fba2be42debfa0b962e172d0962021-12-02T20:14:59ZHierarchical modelling of immunoglobulin coated bacteria in dogs with chronic enteropathy shows reduction in coating with disease remission but marked inter-individual and treatment-response variability.1932-620310.1371/journal.pone.0255012https://doaj.org/article/4e6362fba2be42debfa0b962e172d0962021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255012https://doaj.org/toc/1932-6203Chronic enteropathies are a common problem in dogs, but many aspects of the pathogenesis remain unknown, making the therapeutic approach challenging in some cases. Environmental factors are intimately related to the development and perpetuation of gastrointestinal disease and the gut microbiome has been identified as a contributing factor. Previous studies have identified dysbiosis and reduced bacterial diversity in the gastrointestinal microbiota of dogs with chronic enteropathies. In this case-controlled study, we use flow cytometry and 16S rRNA sequencing to characterise bacteria highly coated with IgA or IgG in faecal samples from dogs with chronic enteropathy and evaluated their correlation with disease and resolution of the clinical signs. IgA and IgG-coated faecal bacterial counts were significantly higher during active disease compared to healthy dogs and decreased with the resolution of the clinical signs. Characterisation of taxa-specific coating of the intestinal microbiota with IgA and IgG showed marked variation between dogs and disease states, and different patterns of immunoglobulin enrichment were observed in dogs with chronic enteropathy, particularly for Erysipelotrichaceae, Clostridicaceae, Enterobacteriaceae, Prevotellaceae and Bacteroidaceae, families. Although, members of these bacterial groups have been associated with strong immunogenic properties and could potentially constitute important biomarkers of disease, their significance and role need to be further investigated.Lina María Martínez-LópezAlexis Perez-GonzalezElizabeth Ann WashingtonAndrew P WoodwardAlexandra Jazmin Roth-SchulzeJulien R S DandrieuxThurid JohnstoneNathalee PrakashAaron JexCaroline MansfieldPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255012 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lina María Martínez-López Alexis Perez-Gonzalez Elizabeth Ann Washington Andrew P Woodward Alexandra Jazmin Roth-Schulze Julien R S Dandrieux Thurid Johnstone Nathalee Prakash Aaron Jex Caroline Mansfield Hierarchical modelling of immunoglobulin coated bacteria in dogs with chronic enteropathy shows reduction in coating with disease remission but marked inter-individual and treatment-response variability.
description	Chronic enteropathies are a common problem in dogs, but many aspects of the pathogenesis remain unknown, making the therapeutic approach challenging in some cases. Environmental factors are intimately related to the development and perpetuation of gastrointestinal disease and the gut microbiome has been identified as a contributing factor. Previous studies have identified dysbiosis and reduced bacterial diversity in the gastrointestinal microbiota of dogs with chronic enteropathies. In this case-controlled study, we use flow cytometry and 16S rRNA sequencing to characterise bacteria highly coated with IgA or IgG in faecal samples from dogs with chronic enteropathy and evaluated their correlation with disease and resolution of the clinical signs. IgA and IgG-coated faecal bacterial counts were significantly higher during active disease compared to healthy dogs and decreased with the resolution of the clinical signs. Characterisation of taxa-specific coating of the intestinal microbiota with IgA and IgG showed marked variation between dogs and disease states, and different patterns of immunoglobulin enrichment were observed in dogs with chronic enteropathy, particularly for Erysipelotrichaceae, Clostridicaceae, Enterobacteriaceae, Prevotellaceae and Bacteroidaceae, families. Although, members of these bacterial groups have been associated with strong immunogenic properties and could potentially constitute important biomarkers of disease, their significance and role need to be further investigated.
format	article
author	Lina María Martínez-López Alexis Perez-Gonzalez Elizabeth Ann Washington Andrew P Woodward Alexandra Jazmin Roth-Schulze Julien R S Dandrieux Thurid Johnstone Nathalee Prakash Aaron Jex Caroline Mansfield
author_facet	Lina María Martínez-López Alexis Perez-Gonzalez Elizabeth Ann Washington Andrew P Woodward Alexandra Jazmin Roth-Schulze Julien R S Dandrieux Thurid Johnstone Nathalee Prakash Aaron Jex Caroline Mansfield
author_sort	Lina María Martínez-López
title	Hierarchical modelling of immunoglobulin coated bacteria in dogs with chronic enteropathy shows reduction in coating with disease remission but marked inter-individual and treatment-response variability.
title_short	Hierarchical modelling of immunoglobulin coated bacteria in dogs with chronic enteropathy shows reduction in coating with disease remission but marked inter-individual and treatment-response variability.
title_full	Hierarchical modelling of immunoglobulin coated bacteria in dogs with chronic enteropathy shows reduction in coating with disease remission but marked inter-individual and treatment-response variability.
title_fullStr	Hierarchical modelling of immunoglobulin coated bacteria in dogs with chronic enteropathy shows reduction in coating with disease remission but marked inter-individual and treatment-response variability.
title_full_unstemmed	Hierarchical modelling of immunoglobulin coated bacteria in dogs with chronic enteropathy shows reduction in coating with disease remission but marked inter-individual and treatment-response variability.
title_sort	hierarchical modelling of immunoglobulin coated bacteria in dogs with chronic enteropathy shows reduction in coating with disease remission but marked inter-individual and treatment-response variability.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/4e6362fba2be42debfa0b962e172d096
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Hierarchical modelling of immunoglobulin coated bacteria in dogs with chronic enteropathy shows reduction in coating with disease remission but marked inter-individual and treatment-response variability.

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