Magnetic resonance colonography assessment of acute trinitrobenzene sulfonic acid colitis in pre-pubertal rats

Pre-pubertal murine models of acute colitis are lacking. Magnetic resonance colonography (MRC) is a promising minimally invasive tool to assess colitis. We aimed to: 1/ Adapt a model of acute experimental colitis to pre-pubertal rats and determine whether MRC characteristics correlate with histologi...

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Autores principales:	Claire Dupont-Lucas, Rachel Marion-Letellier, Mathilde Pala, Charlène Guerin, Christine Bôle-Feysot, Emmeline Salameh, Alexis Goichon, Lionel Nicol, Moutaz Aziz, Céline Savoye-Collet, Guillaume Savoye
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4e68dca516ca44aab5e3adcf2fdca7a5
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spelling	oai:doaj.org-article:4e68dca516ca44aab5e3adcf2fdca7a52021-11-11T07:14:37ZMagnetic resonance colonography assessment of acute trinitrobenzene sulfonic acid colitis in pre-pubertal rats1932-6203https://doaj.org/article/4e68dca516ca44aab5e3adcf2fdca7a52021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568272/?tool=EBIhttps://doaj.org/toc/1932-6203Pre-pubertal murine models of acute colitis are lacking. Magnetic resonance colonography (MRC) is a promising minimally invasive tool to assess colitis. We aimed to: 1/ Adapt a model of acute experimental colitis to pre-pubertal rats and determine whether MRC characteristics correlate with histological inflammation. 2/ Test this model by administering a diet supplemented in transforming growth factor β2 to reverse inflammation. Twenty-four rats were randomized at weaning to one of 3 groups: Trinitrobenzene Sulfonic Acid (TNBS) group (n = 8) fed a standard diet, that received an intra-rectal 60 mg/kg dose of TNBS-ethanol; Control group (n = 8) fed standard diet, that received a dose of intra-rectal PBS; TNBS+MODULEN group (n = 8) that received a dose of TNBS and were exclusively fed MODULEN-IBD® after induction of colitis. One week after induction of colitis, rats were assessed by MRC, colon histopathology and inflammation markers (Interleukin 1β, Tumor necrosis factor α, Nitric Oxide Synthase 2 and Cyclooxygenase 2). TNBS induced typical features of acute colitis on histopathology and MRC (increased colon wall thickness, increased colon intensity on T2-weighted images, target sign, ulcers). Treatment with MODULEN-IBD® did not reduce signs of colitis on MRC. Inflammatory marker expression did not differ among study groups.Claire Dupont-LucasRachel Marion-LetellierMathilde PalaCharlène GuerinChristine Bôle-FeysotEmmeline SalamehAlexis GoichonLionel NicolMoutaz AzizCéline Savoye-ColletGuillaume SavoyePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Claire Dupont-Lucas Rachel Marion-Letellier Mathilde Pala Charlène Guerin Christine Bôle-Feysot Emmeline Salameh Alexis Goichon Lionel Nicol Moutaz Aziz Céline Savoye-Collet Guillaume Savoye Magnetic resonance colonography assessment of acute trinitrobenzene sulfonic acid colitis in pre-pubertal rats
description	Pre-pubertal murine models of acute colitis are lacking. Magnetic resonance colonography (MRC) is a promising minimally invasive tool to assess colitis. We aimed to: 1/ Adapt a model of acute experimental colitis to pre-pubertal rats and determine whether MRC characteristics correlate with histological inflammation. 2/ Test this model by administering a diet supplemented in transforming growth factor β2 to reverse inflammation. Twenty-four rats were randomized at weaning to one of 3 groups: Trinitrobenzene Sulfonic Acid (TNBS) group (n = 8) fed a standard diet, that received an intra-rectal 60 mg/kg dose of TNBS-ethanol; Control group (n = 8) fed standard diet, that received a dose of intra-rectal PBS; TNBS+MODULEN group (n = 8) that received a dose of TNBS and were exclusively fed MODULEN-IBD® after induction of colitis. One week after induction of colitis, rats were assessed by MRC, colon histopathology and inflammation markers (Interleukin 1β, Tumor necrosis factor α, Nitric Oxide Synthase 2 and Cyclooxygenase 2). TNBS induced typical features of acute colitis on histopathology and MRC (increased colon wall thickness, increased colon intensity on T2-weighted images, target sign, ulcers). Treatment with MODULEN-IBD® did not reduce signs of colitis on MRC. Inflammatory marker expression did not differ among study groups.
format	article
author	Claire Dupont-Lucas Rachel Marion-Letellier Mathilde Pala Charlène Guerin Christine Bôle-Feysot Emmeline Salameh Alexis Goichon Lionel Nicol Moutaz Aziz Céline Savoye-Collet Guillaume Savoye
author_facet	Claire Dupont-Lucas Rachel Marion-Letellier Mathilde Pala Charlène Guerin Christine Bôle-Feysot Emmeline Salameh Alexis Goichon Lionel Nicol Moutaz Aziz Céline Savoye-Collet Guillaume Savoye
author_sort	Claire Dupont-Lucas
title	Magnetic resonance colonography assessment of acute trinitrobenzene sulfonic acid colitis in pre-pubertal rats
title_short	Magnetic resonance colonography assessment of acute trinitrobenzene sulfonic acid colitis in pre-pubertal rats
title_full	Magnetic resonance colonography assessment of acute trinitrobenzene sulfonic acid colitis in pre-pubertal rats
title_fullStr	Magnetic resonance colonography assessment of acute trinitrobenzene sulfonic acid colitis in pre-pubertal rats
title_full_unstemmed	Magnetic resonance colonography assessment of acute trinitrobenzene sulfonic acid colitis in pre-pubertal rats
title_sort	magnetic resonance colonography assessment of acute trinitrobenzene sulfonic acid colitis in pre-pubertal rats
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/4e68dca516ca44aab5e3adcf2fdca7a5
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Magnetic resonance colonography assessment of acute trinitrobenzene sulfonic acid colitis in pre-pubertal rats

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