A Two-step Strategy for High-Value-Added Utilization of Rapeseed Meal by Concurrent Improvement of Phenolic Extraction and Protein Conversion for Microbial Iturin A Production
Rapeseed meal (RSM) is a major by-product of oil extraction from rapeseed, consists mainly of proteins and phenolic compounds. The use of RSM as protein feedstock for microbial fermentation is always hampered by phenolic compounds, which have antioxidant property with health-promoting benefits but i...
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2021
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oai:doaj.org-article:4e6c17a7b7ac40ba9426bbdde867587a2021-11-17T07:02:52ZA Two-step Strategy for High-Value-Added Utilization of Rapeseed Meal by Concurrent Improvement of Phenolic Extraction and Protein Conversion for Microbial Iturin A Production2296-418510.3389/fbioe.2021.735714https://doaj.org/article/4e6c17a7b7ac40ba9426bbdde867587a2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.735714/fullhttps://doaj.org/toc/2296-4185Rapeseed meal (RSM) is a major by-product of oil extraction from rapeseed, consists mainly of proteins and phenolic compounds. The use of RSM as protein feedstock for microbial fermentation is always hampered by phenolic compounds, which have antioxidant property with health-promoting benefits but inhibit bacterial growth. However, there is still not any good process that simultaneously improve extraction efficiency of phenolic compounds with conversion efficiency of protein residue into microbial production. Here we established a two-step strategy including fungal pretreatment followed by extraction of phenolic compounds. This could not only increase extraction efficiency and antioxidant property of phenolic compounds by about 2-fold, but also improve conversion efficiency of protein residue into iturin A production by Bacillus amyloliquefaciens CX-20 by about 33%. The antioxidant and antibacterial activities of phenolic extracts were influenced by both total phenolic content and profile, while microbial feedstock value of residue was greatly improved because protein content was increased by ∼5% and phenolic content was decreased by ∼60%. Moreover, this two-step process resulted in isolating more proteins from RSM, bringing iturin A production to 1.95 g/L. In conclusion, high-value-added and graded utilization of phenolic extract and protein residue from RSM with zero waste is realized by a two-step strategy, which combines both benefits of fungal pretreatment and phenolic extraction procedures.Meng WangChen YangChen YangChen YangChen YangJean Marie FrançoisXia WanXia WanXia WanXia WanQianchun DengQianchun DengQianchun DengQianchun DengDanyang FengDanyang FengShiyu DengShiyu DengShouwen ChenFenghong HuangFenghong HuangFenghong HuangFenghong HuangWenchao ChenWenchao ChenWenchao ChenWenchao ChenYangmin GongYangmin GongYangmin GongYangmin GongFrontiers Media S.A.articlerapeseed mealtwo-step strategyfungal pretreatmentphenolic compoundsprotein residuemicrobial iturin A productionBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021) |
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DOAJ |
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EN |
| topic |
rapeseed meal two-step strategy fungal pretreatment phenolic compounds protein residue microbial iturin A production Biotechnology TP248.13-248.65 |
| spellingShingle |
rapeseed meal two-step strategy fungal pretreatment phenolic compounds protein residue microbial iturin A production Biotechnology TP248.13-248.65 Meng Wang Chen Yang Chen Yang Chen Yang Chen Yang Jean Marie François Xia Wan Xia Wan Xia Wan Xia Wan Qianchun Deng Qianchun Deng Qianchun Deng Qianchun Deng Danyang Feng Danyang Feng Shiyu Deng Shiyu Deng Shouwen Chen Fenghong Huang Fenghong Huang Fenghong Huang Fenghong Huang Wenchao Chen Wenchao Chen Wenchao Chen Wenchao Chen Yangmin Gong Yangmin Gong Yangmin Gong Yangmin Gong A Two-step Strategy for High-Value-Added Utilization of Rapeseed Meal by Concurrent Improvement of Phenolic Extraction and Protein Conversion for Microbial Iturin A Production |
| description |
Rapeseed meal (RSM) is a major by-product of oil extraction from rapeseed, consists mainly of proteins and phenolic compounds. The use of RSM as protein feedstock for microbial fermentation is always hampered by phenolic compounds, which have antioxidant property with health-promoting benefits but inhibit bacterial growth. However, there is still not any good process that simultaneously improve extraction efficiency of phenolic compounds with conversion efficiency of protein residue into microbial production. Here we established a two-step strategy including fungal pretreatment followed by extraction of phenolic compounds. This could not only increase extraction efficiency and antioxidant property of phenolic compounds by about 2-fold, but also improve conversion efficiency of protein residue into iturin A production by Bacillus amyloliquefaciens CX-20 by about 33%. The antioxidant and antibacterial activities of phenolic extracts were influenced by both total phenolic content and profile, while microbial feedstock value of residue was greatly improved because protein content was increased by ∼5% and phenolic content was decreased by ∼60%. Moreover, this two-step process resulted in isolating more proteins from RSM, bringing iturin A production to 1.95 g/L. In conclusion, high-value-added and graded utilization of phenolic extract and protein residue from RSM with zero waste is realized by a two-step strategy, which combines both benefits of fungal pretreatment and phenolic extraction procedures. |
| format |
article |
| author |
Meng Wang Chen Yang Chen Yang Chen Yang Chen Yang Jean Marie François Xia Wan Xia Wan Xia Wan Xia Wan Qianchun Deng Qianchun Deng Qianchun Deng Qianchun Deng Danyang Feng Danyang Feng Shiyu Deng Shiyu Deng Shouwen Chen Fenghong Huang Fenghong Huang Fenghong Huang Fenghong Huang Wenchao Chen Wenchao Chen Wenchao Chen Wenchao Chen Yangmin Gong Yangmin Gong Yangmin Gong Yangmin Gong |
| author_facet |
Meng Wang Chen Yang Chen Yang Chen Yang Chen Yang Jean Marie François Xia Wan Xia Wan Xia Wan Xia Wan Qianchun Deng Qianchun Deng Qianchun Deng Qianchun Deng Danyang Feng Danyang Feng Shiyu Deng Shiyu Deng Shouwen Chen Fenghong Huang Fenghong Huang Fenghong Huang Fenghong Huang Wenchao Chen Wenchao Chen Wenchao Chen Wenchao Chen Yangmin Gong Yangmin Gong Yangmin Gong Yangmin Gong |
| author_sort |
Meng Wang |
| title |
A Two-step Strategy for High-Value-Added Utilization of Rapeseed Meal by Concurrent Improvement of Phenolic Extraction and Protein Conversion for Microbial Iturin A Production |
| title_short |
A Two-step Strategy for High-Value-Added Utilization of Rapeseed Meal by Concurrent Improvement of Phenolic Extraction and Protein Conversion for Microbial Iturin A Production |
| title_full |
A Two-step Strategy for High-Value-Added Utilization of Rapeseed Meal by Concurrent Improvement of Phenolic Extraction and Protein Conversion for Microbial Iturin A Production |
| title_fullStr |
A Two-step Strategy for High-Value-Added Utilization of Rapeseed Meal by Concurrent Improvement of Phenolic Extraction and Protein Conversion for Microbial Iturin A Production |
| title_full_unstemmed |
A Two-step Strategy for High-Value-Added Utilization of Rapeseed Meal by Concurrent Improvement of Phenolic Extraction and Protein Conversion for Microbial Iturin A Production |
| title_sort |
two-step strategy for high-value-added utilization of rapeseed meal by concurrent improvement of phenolic extraction and protein conversion for microbial iturin a production |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/4e6c17a7b7ac40ba9426bbdde867587a |
| work_keys_str_mv |
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