Mapping physiological inputs from multiple photoreceptor systems to dopaminergic amacrine cells in the mouse retina

Abstract In the vertebrate retina, dopamine is synthesized and released by a specialized type of amacrine cell, the dopaminergic amacrine cell (DAC). DAC activity is stimulated by rods, cones, and melanopsin-expressing intrinsically photosensitive retinal ganglion cells upon illumination. However, t...

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Autores principales:	Xiwu Zhao, Kwoon Y. Wong, Dao-Qi Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4e6f76bec27a4d46850fff89cae1b503
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spelling	oai:doaj.org-article:4e6f76bec27a4d46850fff89cae1b5032021-12-02T16:07:59ZMapping physiological inputs from multiple photoreceptor systems to dopaminergic amacrine cells in the mouse retina10.1038/s41598-017-08172-x2045-2322https://doaj.org/article/4e6f76bec27a4d46850fff89cae1b5032017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08172-xhttps://doaj.org/toc/2045-2322Abstract In the vertebrate retina, dopamine is synthesized and released by a specialized type of amacrine cell, the dopaminergic amacrine cell (DAC). DAC activity is stimulated by rods, cones, and melanopsin-expressing intrinsically photosensitive retinal ganglion cells upon illumination. However, the relative contributions of these three photoreceptor systems to the DAC light-induced response are unknown. Here we found that rods excite dark-adapted DACs across a wide range of stimulation intensities, primarily through connexin-36-dependent rod pathways. Similar rod-driven responses were observed in both ventral and dorsal DACs. We further found that in the dorsal retina, M-cones and melanopsin contribute to dark-adapted DAC responses with a similar threshold intensity. In the ventral retina, however, the threshold intensity for M-cone-driven responses was two log units greater than that observed in dorsal DACs, and melanopsin-driven responses were almost undetectable. We also examined the DAC response to prolonged adapting light and found such responses to be mediated by rods under dim lighting conditions, rods/M-cones/melanopsin under intermediate lighting conditions, and cones and melanopsin under bright lighting conditions. Our results elucidate the relative contributions of the three photoreceptor systems to DACs under different lighting conditions, furthering our understanding of the role these cells play in the visual system.Xiwu ZhaoKwoon Y. WongDao-Qi ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiwu Zhao Kwoon Y. Wong Dao-Qi Zhang Mapping physiological inputs from multiple photoreceptor systems to dopaminergic amacrine cells in the mouse retina
description	Abstract In the vertebrate retina, dopamine is synthesized and released by a specialized type of amacrine cell, the dopaminergic amacrine cell (DAC). DAC activity is stimulated by rods, cones, and melanopsin-expressing intrinsically photosensitive retinal ganglion cells upon illumination. However, the relative contributions of these three photoreceptor systems to the DAC light-induced response are unknown. Here we found that rods excite dark-adapted DACs across a wide range of stimulation intensities, primarily through connexin-36-dependent rod pathways. Similar rod-driven responses were observed in both ventral and dorsal DACs. We further found that in the dorsal retina, M-cones and melanopsin contribute to dark-adapted DAC responses with a similar threshold intensity. In the ventral retina, however, the threshold intensity for M-cone-driven responses was two log units greater than that observed in dorsal DACs, and melanopsin-driven responses were almost undetectable. We also examined the DAC response to prolonged adapting light and found such responses to be mediated by rods under dim lighting conditions, rods/M-cones/melanopsin under intermediate lighting conditions, and cones and melanopsin under bright lighting conditions. Our results elucidate the relative contributions of the three photoreceptor systems to DACs under different lighting conditions, furthering our understanding of the role these cells play in the visual system.
format	article
author	Xiwu Zhao Kwoon Y. Wong Dao-Qi Zhang
author_facet	Xiwu Zhao Kwoon Y. Wong Dao-Qi Zhang
author_sort	Xiwu Zhao
title	Mapping physiological inputs from multiple photoreceptor systems to dopaminergic amacrine cells in the mouse retina
title_short	Mapping physiological inputs from multiple photoreceptor systems to dopaminergic amacrine cells in the mouse retina
title_full	Mapping physiological inputs from multiple photoreceptor systems to dopaminergic amacrine cells in the mouse retina
title_fullStr	Mapping physiological inputs from multiple photoreceptor systems to dopaminergic amacrine cells in the mouse retina
title_full_unstemmed	Mapping physiological inputs from multiple photoreceptor systems to dopaminergic amacrine cells in the mouse retina
title_sort	mapping physiological inputs from multiple photoreceptor systems to dopaminergic amacrine cells in the mouse retina
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4e6f76bec27a4d46850fff89cae1b503
work_keys_str_mv	AT xiwuzhao mappingphysiologicalinputsfrommultiplephotoreceptorsystemstodopaminergicamacrinecellsinthemouseretina AT kwoonywong mappingphysiologicalinputsfrommultiplephotoreceptorsystemstodopaminergicamacrinecellsinthemouseretina AT daoqizhang mappingphysiologicalinputsfrommultiplephotoreceptorsystemstodopaminergicamacrinecellsinthemouseretina
_version_	1718384654003732480

Mapping physiological inputs from multiple photoreceptor systems to dopaminergic amacrine cells in the mouse retina

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