CD133: enhancement of bone healing by local transplantation of peripheral blood cells in a biologically delayed rat osteotomy model.

Sufficient angiogenesis is crucial during tissue regeneration and therefore also pivotal in bone defect healing. Recently, peripheral blood derived progenitor cells have been identified to have in addition to their angiogenic potential also osteogenic characteristics, leading to the hypothesis that...

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Autores principales: Bernd Preininger, Georg Duda, Hinnerk Gerigk, Jonas Bruckner, Agnes Ellinghaus, F Andrea Sass, Carsten Perka, Katharina Schmidt-Bleek, Anke Dienelt
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Publicado: Public Library of Science (PLoS) 2013
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Acceso en línea:https://doaj.org/article/4e7cf79915de492d84e4943122a64a70
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spelling oai:doaj.org-article:4e7cf79915de492d84e4943122a64a702021-11-18T07:57:33ZCD133: enhancement of bone healing by local transplantation of peripheral blood cells in a biologically delayed rat osteotomy model.1932-620310.1371/journal.pone.0052650https://doaj.org/article/4e7cf79915de492d84e4943122a64a702013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23457441/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Sufficient angiogenesis is crucial during tissue regeneration and therefore also pivotal in bone defect healing. Recently, peripheral blood derived progenitor cells have been identified to have in addition to their angiogenic potential also osteogenic characteristics, leading to the hypothesis that bone regeneration could be stimulated by local administration of these cells. The aim of this study was to evaluate the angiogenic potential of locally administered progenitor cells to improve bone defect healing. Cells were separated from the peripheral blood of donor animals using the markers CD34 and CD133. Results of the in vitro experiments confirmed high angiogenic potential in the CD133(+) cell group. CD34(+) and CD133(+) cells were tested in an in vivo rat femoral defect model of delayed healing for their positive effect on the healing outcome. An increased callus formation and higher bone mineral density of callus tissue was found after the CD133(+) cell treatment compared to the group treated with CD34(+) cells and the control group without cells. Histological findings confirmed an increase in vessel formation and mineralization at day 42 in the osteotomy gap after CD133(+) cell transplantation. The higher angiogenic potential of CD133(+) cells from the in vitro experients therefore correlates with the in vivo data. This study demonstrates the suitability of angiogenic precursors to further bone healing and gives an indication that peripheral blood is a promising source for progenitor cells circumventing the problems associated with bone marrow extraction.Bernd PreiningerGeorg DudaHinnerk GerigkJonas BrucknerAgnes EllinghausF Andrea SassCarsten PerkaKatharina Schmidt-BleekAnke DieneltPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 2, p e52650 (2013)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Bernd Preininger
Georg Duda
Hinnerk Gerigk
Jonas Bruckner
Agnes Ellinghaus
F Andrea Sass
Carsten Perka
Katharina Schmidt-Bleek
Anke Dienelt
CD133: enhancement of bone healing by local transplantation of peripheral blood cells in a biologically delayed rat osteotomy model.
description Sufficient angiogenesis is crucial during tissue regeneration and therefore also pivotal in bone defect healing. Recently, peripheral blood derived progenitor cells have been identified to have in addition to their angiogenic potential also osteogenic characteristics, leading to the hypothesis that bone regeneration could be stimulated by local administration of these cells. The aim of this study was to evaluate the angiogenic potential of locally administered progenitor cells to improve bone defect healing. Cells were separated from the peripheral blood of donor animals using the markers CD34 and CD133. Results of the in vitro experiments confirmed high angiogenic potential in the CD133(+) cell group. CD34(+) and CD133(+) cells were tested in an in vivo rat femoral defect model of delayed healing for their positive effect on the healing outcome. An increased callus formation and higher bone mineral density of callus tissue was found after the CD133(+) cell treatment compared to the group treated with CD34(+) cells and the control group without cells. Histological findings confirmed an increase in vessel formation and mineralization at day 42 in the osteotomy gap after CD133(+) cell transplantation. The higher angiogenic potential of CD133(+) cells from the in vitro experients therefore correlates with the in vivo data. This study demonstrates the suitability of angiogenic precursors to further bone healing and gives an indication that peripheral blood is a promising source for progenitor cells circumventing the problems associated with bone marrow extraction.
format article
author Bernd Preininger
Georg Duda
Hinnerk Gerigk
Jonas Bruckner
Agnes Ellinghaus
F Andrea Sass
Carsten Perka
Katharina Schmidt-Bleek
Anke Dienelt
author_facet Bernd Preininger
Georg Duda
Hinnerk Gerigk
Jonas Bruckner
Agnes Ellinghaus
F Andrea Sass
Carsten Perka
Katharina Schmidt-Bleek
Anke Dienelt
author_sort Bernd Preininger
title CD133: enhancement of bone healing by local transplantation of peripheral blood cells in a biologically delayed rat osteotomy model.
title_short CD133: enhancement of bone healing by local transplantation of peripheral blood cells in a biologically delayed rat osteotomy model.
title_full CD133: enhancement of bone healing by local transplantation of peripheral blood cells in a biologically delayed rat osteotomy model.
title_fullStr CD133: enhancement of bone healing by local transplantation of peripheral blood cells in a biologically delayed rat osteotomy model.
title_full_unstemmed CD133: enhancement of bone healing by local transplantation of peripheral blood cells in a biologically delayed rat osteotomy model.
title_sort cd133: enhancement of bone healing by local transplantation of peripheral blood cells in a biologically delayed rat osteotomy model.
publisher Public Library of Science (PLoS)
publishDate 2013
url https://doaj.org/article/4e7cf79915de492d84e4943122a64a70
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