Functional role of PilA in iron acquisition in the cyanobacterium Synechocystis sp. PCC 6803.

Cyanobacteria require large quantities of iron to maintain their photosynthetic machinery; however, in most environments iron is present in the form of insoluble iron oxides. Whether cyanobacteria can utilize these sources of iron, and the potential molecular mechanisms involved remains to be define...

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Autores principales:	Jacob J Lamb, Ryan E Hill, Julian J Eaton-Rye, Martin F Hohmann-Marriott
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4e85b320979c4eb79d9a5f92e6914e2a
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spelling	oai:doaj.org-article:4e85b320979c4eb79d9a5f92e6914e2a2021-11-25T06:03:09ZFunctional role of PilA in iron acquisition in the cyanobacterium Synechocystis sp. PCC 6803.1932-620310.1371/journal.pone.0105761https://doaj.org/article/4e85b320979c4eb79d9a5f92e6914e2a2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25157828/?tool=EBIhttps://doaj.org/toc/1932-6203Cyanobacteria require large quantities of iron to maintain their photosynthetic machinery; however, in most environments iron is present in the form of insoluble iron oxides. Whether cyanobacteria can utilize these sources of iron, and the potential molecular mechanisms involved remains to be defined. There is increasing evidence that pili can facilitate electron donation to extracellular electron acceptors, like iron oxides in non-photosynthetic bacteria. In these organisms, the donation of electrons to iron oxides is thought to be crucial for maintaining respiration in the absence of oxygen. Our study investigates if PilA1 (major pilin protein) may also provide a mechanism to convert insoluble ferric iron into soluble ferrous iron. Growth experiments supported by spectroscopic data of a strain deficient in pilA1 indicate that the presence of the pilA1 gene enhances the ability to grow on iron oxides. These observations suggest a novel function of PilA1 in cyanobacterial iron acquisition.Jacob J LambRyan E HillJulian J Eaton-RyeMartin F Hohmann-MarriottPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e105761 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jacob J Lamb Ryan E Hill Julian J Eaton-Rye Martin F Hohmann-Marriott Functional role of PilA in iron acquisition in the cyanobacterium Synechocystis sp. PCC 6803.
description	Cyanobacteria require large quantities of iron to maintain their photosynthetic machinery; however, in most environments iron is present in the form of insoluble iron oxides. Whether cyanobacteria can utilize these sources of iron, and the potential molecular mechanisms involved remains to be defined. There is increasing evidence that pili can facilitate electron donation to extracellular electron acceptors, like iron oxides in non-photosynthetic bacteria. In these organisms, the donation of electrons to iron oxides is thought to be crucial for maintaining respiration in the absence of oxygen. Our study investigates if PilA1 (major pilin protein) may also provide a mechanism to convert insoluble ferric iron into soluble ferrous iron. Growth experiments supported by spectroscopic data of a strain deficient in pilA1 indicate that the presence of the pilA1 gene enhances the ability to grow on iron oxides. These observations suggest a novel function of PilA1 in cyanobacterial iron acquisition.
format	article
author	Jacob J Lamb Ryan E Hill Julian J Eaton-Rye Martin F Hohmann-Marriott
author_facet	Jacob J Lamb Ryan E Hill Julian J Eaton-Rye Martin F Hohmann-Marriott
author_sort	Jacob J Lamb
title	Functional role of PilA in iron acquisition in the cyanobacterium Synechocystis sp. PCC 6803.
title_short	Functional role of PilA in iron acquisition in the cyanobacterium Synechocystis sp. PCC 6803.
title_full	Functional role of PilA in iron acquisition in the cyanobacterium Synechocystis sp. PCC 6803.
title_fullStr	Functional role of PilA in iron acquisition in the cyanobacterium Synechocystis sp. PCC 6803.
title_full_unstemmed	Functional role of PilA in iron acquisition in the cyanobacterium Synechocystis sp. PCC 6803.
title_sort	functional role of pila in iron acquisition in the cyanobacterium synechocystis sp. pcc 6803.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/4e85b320979c4eb79d9a5f92e6914e2a
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Functional role of PilA in iron acquisition in the cyanobacterium Synechocystis sp. PCC 6803.

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