A New MiRNA MiRm0002 in Eggplant Participates in the Regulation of Defense Responses to Verticillium Wilt

Verticillium wilt is a major disease that severely affects eggplant production, and a new eggplant miRNA named miRm0002 identified through high-throughput sequencing was highly induced by Verticillium wilt infection. However, the miRm0002 function was still elusive. In this study, the sequence of th...

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Autores principales:	Wenjiao Zhu, Xinru Liu, Min Chen, Nianjiao Tao, Alexander Tendu, Qing Yang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Solanum melongena</i> L. miRm0002 Verticillium wilt defense response Botany QK1-989
Acceso en línea:	https://doaj.org/article/4e8a70e6052e44f9b69c927066e2c0d0
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spelling	oai:doaj.org-article:4e8a70e6052e44f9b69c927066e2c0d02021-11-25T18:44:39ZA New MiRNA MiRm0002 in Eggplant Participates in the Regulation of Defense Responses to Verticillium Wilt10.3390/plants101122742223-7747https://doaj.org/article/4e8a70e6052e44f9b69c927066e2c0d02021-10-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2274https://doaj.org/toc/2223-7747Verticillium wilt is a major disease that severely affects eggplant production, and a new eggplant miRNA named miRm0002 identified through high-throughput sequencing was highly induced by Verticillium wilt infection. However, the miRm0002 function was still elusive. In this study, the sequence of the miRm0002 precursor was cloned and transgenic eggplants were constructed. In vivo inoculation test and in vitro fungistatic test showed that overexpressing miRm0002 lines were more resistant to <i>Verticillium dahliae</i> and inhibiting miRm0002 lines were more sensitive, compared to the wild-type (WT) control. Some physiological indicators were selected and the results showed that SOD, POD, and CAT activities were significantly increased in Verticillium wilt-infected overexpressing miRm0002 lines, indicating that the expression of miRm0002 activates the antioxidant system. QRT-PCR assay showed that the transcript expression of miRm0002 candidate target <i>ARF8</i>, a gene encoding auxin response factor was negatively related to miRm0002 in WT as well as transgenic eggplants. However, RLM-RACE mapping and degradome sequencing showed miRm0002 could not cleave the sequence of <i>ARF8</i>. Taken together, these data suggest that miRm0002 plays a positive role in the defense response of eggplant against Verticillium wilt.Wenjiao ZhuXinru LiuMin ChenNianjiao TaoAlexander TenduQing YangMDPI AGarticle<i>Solanum melongena</i> L.miRm0002Verticillium wiltdefense responseBotanyQK1-989ENPlants, Vol 10, Iss 2274, p 2274 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Solanum melongena</i> L. miRm0002 Verticillium wilt defense response Botany QK1-989
spellingShingle	<i>Solanum melongena</i> L. miRm0002 Verticillium wilt defense response Botany QK1-989 Wenjiao Zhu Xinru Liu Min Chen Nianjiao Tao Alexander Tendu Qing Yang A New MiRNA MiRm0002 in Eggplant Participates in the Regulation of Defense Responses to Verticillium Wilt
description	Verticillium wilt is a major disease that severely affects eggplant production, and a new eggplant miRNA named miRm0002 identified through high-throughput sequencing was highly induced by Verticillium wilt infection. However, the miRm0002 function was still elusive. In this study, the sequence of the miRm0002 precursor was cloned and transgenic eggplants were constructed. In vivo inoculation test and in vitro fungistatic test showed that overexpressing miRm0002 lines were more resistant to <i>Verticillium dahliae</i> and inhibiting miRm0002 lines were more sensitive, compared to the wild-type (WT) control. Some physiological indicators were selected and the results showed that SOD, POD, and CAT activities were significantly increased in Verticillium wilt-infected overexpressing miRm0002 lines, indicating that the expression of miRm0002 activates the antioxidant system. QRT-PCR assay showed that the transcript expression of miRm0002 candidate target <i>ARF8</i>, a gene encoding auxin response factor was negatively related to miRm0002 in WT as well as transgenic eggplants. However, RLM-RACE mapping and degradome sequencing showed miRm0002 could not cleave the sequence of <i>ARF8</i>. Taken together, these data suggest that miRm0002 plays a positive role in the defense response of eggplant against Verticillium wilt.
format	article
author	Wenjiao Zhu Xinru Liu Min Chen Nianjiao Tao Alexander Tendu Qing Yang
author_facet	Wenjiao Zhu Xinru Liu Min Chen Nianjiao Tao Alexander Tendu Qing Yang
author_sort	Wenjiao Zhu
title	A New MiRNA MiRm0002 in Eggplant Participates in the Regulation of Defense Responses to Verticillium Wilt
title_short	A New MiRNA MiRm0002 in Eggplant Participates in the Regulation of Defense Responses to Verticillium Wilt
title_full	A New MiRNA MiRm0002 in Eggplant Participates in the Regulation of Defense Responses to Verticillium Wilt
title_fullStr	A New MiRNA MiRm0002 in Eggplant Participates in the Regulation of Defense Responses to Verticillium Wilt
title_full_unstemmed	A New MiRNA MiRm0002 in Eggplant Participates in the Regulation of Defense Responses to Verticillium Wilt
title_sort	new mirna mirm0002 in eggplant participates in the regulation of defense responses to verticillium wilt
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4e8a70e6052e44f9b69c927066e2c0d0
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A New MiRNA MiRm0002 in Eggplant Participates in the Regulation of Defense Responses to Verticillium Wilt

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