A Phase-Variable Surface Layer from the Gut Symbiont <named-content content-type="genus-species">Bacteroides thetaiotaomicron</named-content>

ABSTRACT The capsule from Bacteroides, a common gut symbiont, has long been a model system for studying the molecular mechanisms of host-symbiont interactions. The Bacteroides capsule is thought to consist of an array of phase-variable polysaccharides that give rise to subpopulations with distinct c...

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Autores principales:	Mao Taketani, Mohamed S. Donia, Amy N. Jacobson, John D. Lambris, Michael A. Fischbach
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2015
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4e9cf846ce3f4ed6a9cd583304c30560
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spelling	oai:doaj.org-article:4e9cf846ce3f4ed6a9cd583304c305602021-11-15T15:41:30ZA Phase-Variable Surface Layer from the Gut Symbiont <named-content content-type="genus-species">Bacteroides thetaiotaomicron</named-content>10.1128/mBio.01339-152150-7511https://doaj.org/article/4e9cf846ce3f4ed6a9cd583304c305602015-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01339-15https://doaj.org/toc/2150-7511ABSTRACT The capsule from Bacteroides, a common gut symbiont, has long been a model system for studying the molecular mechanisms of host-symbiont interactions. The Bacteroides capsule is thought to consist of an array of phase-variable polysaccharides that give rise to subpopulations with distinct cell surface structures. Here, we report the serendipitous discovery of a previously unknown surface structure in Bacteroides thetaiotaomicron: a surface layer composed of a protein of unknown function, BT1927. BT1927, which is expressed in a phase-variable manner by ~1:1,000 cells in a wild-type culture, forms a hexagonally tessellated surface layer. The BT1927-expressing subpopulation is profoundly resistant to complement-mediated killing, due in part to the BT1927-mediated blockade of C3b deposition. Our results show that the Bacteroides surface structure is capable of a far greater degree of structural variation than previously known, and they suggest that structural variation within a Bacteroides species is important for productive gut colonization. IMPORTANCE Many bacterial species elaborate a capsule, a structure that resides outside the cell wall and mediates microbe-microbe and microbe-host interactions. Species of Bacteroides, the most abundant genus in the human gut, produce a capsule that consists of an array of polysaccharides, some of which are known to mediate interactions with the host immune system. Here, we report the discovery of a previously unknown surface structure in Bacteroides thetaiotaomicron. We show that this protein-based structure is expressed by a subset of cells in a population and protects Bacteroides from killing by complement, a component of the innate immune system. This novel surface layer protein is conserved across many species of the genus Bacteroides, suggesting an important role in colonization and host immune modulation.Mao TaketaniMohamed S. DoniaAmy N. JacobsonJohn D. LambrisMichael A. FischbachAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 6, Iss 5 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Mao Taketani Mohamed S. Donia Amy N. Jacobson John D. Lambris Michael A. Fischbach A Phase-Variable Surface Layer from the Gut Symbiont <named-content content-type="genus-species">Bacteroides thetaiotaomicron</named-content>
description	ABSTRACT The capsule from Bacteroides, a common gut symbiont, has long been a model system for studying the molecular mechanisms of host-symbiont interactions. The Bacteroides capsule is thought to consist of an array of phase-variable polysaccharides that give rise to subpopulations with distinct cell surface structures. Here, we report the serendipitous discovery of a previously unknown surface structure in Bacteroides thetaiotaomicron: a surface layer composed of a protein of unknown function, BT1927. BT1927, which is expressed in a phase-variable manner by ~1:1,000 cells in a wild-type culture, forms a hexagonally tessellated surface layer. The BT1927-expressing subpopulation is profoundly resistant to complement-mediated killing, due in part to the BT1927-mediated blockade of C3b deposition. Our results show that the Bacteroides surface structure is capable of a far greater degree of structural variation than previously known, and they suggest that structural variation within a Bacteroides species is important for productive gut colonization. IMPORTANCE Many bacterial species elaborate a capsule, a structure that resides outside the cell wall and mediates microbe-microbe and microbe-host interactions. Species of Bacteroides, the most abundant genus in the human gut, produce a capsule that consists of an array of polysaccharides, some of which are known to mediate interactions with the host immune system. Here, we report the discovery of a previously unknown surface structure in Bacteroides thetaiotaomicron. We show that this protein-based structure is expressed by a subset of cells in a population and protects Bacteroides from killing by complement, a component of the innate immune system. This novel surface layer protein is conserved across many species of the genus Bacteroides, suggesting an important role in colonization and host immune modulation.
format	article
author	Mao Taketani Mohamed S. Donia Amy N. Jacobson John D. Lambris Michael A. Fischbach
author_facet	Mao Taketani Mohamed S. Donia Amy N. Jacobson John D. Lambris Michael A. Fischbach
author_sort	Mao Taketani
title	A Phase-Variable Surface Layer from the Gut Symbiont <named-content content-type="genus-species">Bacteroides thetaiotaomicron</named-content>
title_short	A Phase-Variable Surface Layer from the Gut Symbiont <named-content content-type="genus-species">Bacteroides thetaiotaomicron</named-content>
title_full	A Phase-Variable Surface Layer from the Gut Symbiont <named-content content-type="genus-species">Bacteroides thetaiotaomicron</named-content>
title_fullStr	A Phase-Variable Surface Layer from the Gut Symbiont <named-content content-type="genus-species">Bacteroides thetaiotaomicron</named-content>
title_full_unstemmed	A Phase-Variable Surface Layer from the Gut Symbiont <named-content content-type="genus-species">Bacteroides thetaiotaomicron</named-content>
title_sort	phase-variable surface layer from the gut symbiont <named-content content-type="genus-species">bacteroides thetaiotaomicron</named-content>
publisher	American Society for Microbiology
publishDate	2015
url	https://doaj.org/article/4e9cf846ce3f4ed6a9cd583304c30560
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A Phase-Variable Surface Layer from the Gut Symbiont <named-content content-type="genus-species">Bacteroides thetaiotaomicron</named-content>

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