Preventive training does not interfere with mRNA-encoding myosin and collagen expression during pulmonary arterial hypertension.

To gain insight on the impact of preventive exercise during pulmonary arterial hypertension (PAH), we evaluated the gene expression of myosins and gene-encoding proteins associated with the extracellular matrix remodeling of right hypertrophied ventricles. We used 32 male Wistar rats, separated in f...

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Autores principales:	Thaoan Bruno Mariano, Anthony César de Souza Castilho, Ana Karenina Dias de Almeida Sabela, André Casanova de Oliveira, Sarah Santiloni Cury, Andreo Fernando Aguiar, Raisa de Jesus Dutra Dias, Antonio Carlos Cicogna, Katashi Okoshi, Luis Antonio Justulin Junior, Robson Francisco Carvalho, Francis Lopes Pacagnelli
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4ea639c00c004149ba1a99d89027a7cf
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spelling	oai:doaj.org-article:4ea639c00c004149ba1a99d89027a7cf2021-12-02T20:06:21ZPreventive training does not interfere with mRNA-encoding myosin and collagen expression during pulmonary arterial hypertension.1932-620310.1371/journal.pone.0244768https://doaj.org/article/4ea639c00c004149ba1a99d89027a7cf2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0244768https://doaj.org/toc/1932-6203To gain insight on the impact of preventive exercise during pulmonary arterial hypertension (PAH), we evaluated the gene expression of myosins and gene-encoding proteins associated with the extracellular matrix remodeling of right hypertrophied ventricles. We used 32 male Wistar rats, separated in four groups: Sedentary Control (S, n = 8); Control with Training (T, n = 8); Sedentary with Pulmonary Arterial Hypertension (SPAH, n = 8); and Pulmonary Arterial Hypertension with Training (TPAH, n = 8). All rats underwent a two-week adaptation period; T and TPAH group rats then proceeded to an eight-week training period on a treadmill. At the beginning of the 11th week, S and T groups received an intraperitoneal injection of saline, and SPAH and TPAH groups received an injection of monocrotaline (60 mg/kg). Rats in the T and TPAH groups then continued with the training protocol until the 13th week. We assessed exercise capacity, echocardiography analysis, Fulton's index, cross-sectional areas of cardiomyocytes, collagen content and types, and fractal dimension (FD). Transcript abundance of myosins and extracellular matrix genes were estimated through reverse transcription-quantitative PCR (RT-qPCR). When compared to the SPAH group, the TPAH group showed increases in functional capacity and pulmonary artery acceleration time/pulmonary ejection time ratio and decreases in Fulton's index and cross-sectional areas of myocyte cells. However, preventive exercise did not induce alterations in col1a1 and myh7 gene expression. Our findings demonstrate that preventive exercise improved functional capacity, reduced cardiac hypertrophy, and attenuated PH development without interfering in mRNA-encoding myosin and collagen expression during PAH.Thaoan Bruno MarianoAnthony César de Souza CastilhoAna Karenina Dias de Almeida SabelaAndré Casanova de OliveiraSarah Santiloni CuryAndreo Fernando AguiarRaisa de Jesus Dutra DiasAntonio Carlos CicognaKatashi OkoshiLuis Antonio Justulin JuniorRobson Francisco CarvalhoFrancis Lopes PacagnelliPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0244768 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thaoan Bruno Mariano Anthony César de Souza Castilho Ana Karenina Dias de Almeida Sabela André Casanova de Oliveira Sarah Santiloni Cury Andreo Fernando Aguiar Raisa de Jesus Dutra Dias Antonio Carlos Cicogna Katashi Okoshi Luis Antonio Justulin Junior Robson Francisco Carvalho Francis Lopes Pacagnelli Preventive training does not interfere with mRNA-encoding myosin and collagen expression during pulmonary arterial hypertension.
description	To gain insight on the impact of preventive exercise during pulmonary arterial hypertension (PAH), we evaluated the gene expression of myosins and gene-encoding proteins associated with the extracellular matrix remodeling of right hypertrophied ventricles. We used 32 male Wistar rats, separated in four groups: Sedentary Control (S, n = 8); Control with Training (T, n = 8); Sedentary with Pulmonary Arterial Hypertension (SPAH, n = 8); and Pulmonary Arterial Hypertension with Training (TPAH, n = 8). All rats underwent a two-week adaptation period; T and TPAH group rats then proceeded to an eight-week training period on a treadmill. At the beginning of the 11th week, S and T groups received an intraperitoneal injection of saline, and SPAH and TPAH groups received an injection of monocrotaline (60 mg/kg). Rats in the T and TPAH groups then continued with the training protocol until the 13th week. We assessed exercise capacity, echocardiography analysis, Fulton's index, cross-sectional areas of cardiomyocytes, collagen content and types, and fractal dimension (FD). Transcript abundance of myosins and extracellular matrix genes were estimated through reverse transcription-quantitative PCR (RT-qPCR). When compared to the SPAH group, the TPAH group showed increases in functional capacity and pulmonary artery acceleration time/pulmonary ejection time ratio and decreases in Fulton's index and cross-sectional areas of myocyte cells. However, preventive exercise did not induce alterations in col1a1 and myh7 gene expression. Our findings demonstrate that preventive exercise improved functional capacity, reduced cardiac hypertrophy, and attenuated PH development without interfering in mRNA-encoding myosin and collagen expression during PAH.
format	article
author	Thaoan Bruno Mariano Anthony César de Souza Castilho Ana Karenina Dias de Almeida Sabela André Casanova de Oliveira Sarah Santiloni Cury Andreo Fernando Aguiar Raisa de Jesus Dutra Dias Antonio Carlos Cicogna Katashi Okoshi Luis Antonio Justulin Junior Robson Francisco Carvalho Francis Lopes Pacagnelli
author_facet	Thaoan Bruno Mariano Anthony César de Souza Castilho Ana Karenina Dias de Almeida Sabela André Casanova de Oliveira Sarah Santiloni Cury Andreo Fernando Aguiar Raisa de Jesus Dutra Dias Antonio Carlos Cicogna Katashi Okoshi Luis Antonio Justulin Junior Robson Francisco Carvalho Francis Lopes Pacagnelli
author_sort	Thaoan Bruno Mariano
title	Preventive training does not interfere with mRNA-encoding myosin and collagen expression during pulmonary arterial hypertension.
title_short	Preventive training does not interfere with mRNA-encoding myosin and collagen expression during pulmonary arterial hypertension.
title_full	Preventive training does not interfere with mRNA-encoding myosin and collagen expression during pulmonary arterial hypertension.
title_fullStr	Preventive training does not interfere with mRNA-encoding myosin and collagen expression during pulmonary arterial hypertension.
title_full_unstemmed	Preventive training does not interfere with mRNA-encoding myosin and collagen expression during pulmonary arterial hypertension.
title_sort	preventive training does not interfere with mrna-encoding myosin and collagen expression during pulmonary arterial hypertension.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/4ea639c00c004149ba1a99d89027a7cf
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Preventive training does not interfere with mRNA-encoding myosin and collagen expression during pulmonary arterial hypertension.

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