Novel Biological and Molecular Characterization in Radiopharmaceutical Preclinical Design

In this study, the potential of a digital autoradiography system equipped with a super resolution screen has been evaluated to investigate the biodistribution of a 18F-PSMA inhibitor in a prostate cancer mouse model. Twelve double xenograft NOD/SCID mice (LNCAP and PC3 tumours) were divided into thr...

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Autores principales:	Nicoletta Urbano, Manuel Scimeca, Anna Tolomeo, Vincenzo Dimiccoli, Elena Bonanno, Orazio Schillaci
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	nuclear imaging pre-clinical model radiopharmaceutical digital autoradiography system Medicine R
Acceso en línea:	https://doaj.org/article/4ea74e1fb08b448ca4a70a8a0ddcf136
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spelling	oai:doaj.org-article:4ea74e1fb08b448ca4a70a8a0ddcf1362021-11-11T17:30:53ZNovel Biological and Molecular Characterization in Radiopharmaceutical Preclinical Design10.3390/jcm102148502077-0383https://doaj.org/article/4ea74e1fb08b448ca4a70a8a0ddcf1362021-10-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/21/4850https://doaj.org/toc/2077-0383In this study, the potential of a digital autoradiography system equipped with a super resolution screen has been evaluated to investigate the biodistribution of a 18F-PSMA inhibitor in a prostate cancer mouse model. Twelve double xenograft NOD/SCID mice (LNCAP and PC3 tumours) were divided into three groups according to post-injection time points of an 18F-PSMA inhibitor. Groups of 4 mice were used to evaluate the biodistribution of the radiopharmaceutical after 30-, 60- and 120-min post-injection. Data here reported demonstrated that the digital autoradiography system is suitable to analyse the biodistribution of an 18F-PSMA inhibitor in both whole small-animal bodies and in single organs. The exposure of both whole mouse bodies and organs on the super resolution screen surface allowed the radioactivity of the PSMA inhibitor distributed in the tissues to be detected and quantified. Data obtained by using a digital autoradiography system were in line with the values detected by the activity calibrator. In addition, the image obtained from the super resolution screen allowed a perfect overlap with the tumour images achieved under the optical microscope. In conclusion, biodistribution studies performed by the autoradiography system allow the microscopical modifications induced by therapeutic radiopharmaceuticals to be studied by comparing the molecular imaging and histopathological data at the sub-cellular level.Nicoletta UrbanoManuel ScimecaAnna TolomeoVincenzo DimiccoliElena BonannoOrazio SchillaciMDPI AGarticlenuclear imagingpre-clinical modelradiopharmaceuticaldigital autoradiography systemMedicineRENJournal of Clinical Medicine, Vol 10, Iss 4850, p 4850 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nuclear imaging pre-clinical model radiopharmaceutical digital autoradiography system Medicine R
spellingShingle	nuclear imaging pre-clinical model radiopharmaceutical digital autoradiography system Medicine R Nicoletta Urbano Manuel Scimeca Anna Tolomeo Vincenzo Dimiccoli Elena Bonanno Orazio Schillaci Novel Biological and Molecular Characterization in Radiopharmaceutical Preclinical Design
description	In this study, the potential of a digital autoradiography system equipped with a super resolution screen has been evaluated to investigate the biodistribution of a 18F-PSMA inhibitor in a prostate cancer mouse model. Twelve double xenograft NOD/SCID mice (LNCAP and PC3 tumours) were divided into three groups according to post-injection time points of an 18F-PSMA inhibitor. Groups of 4 mice were used to evaluate the biodistribution of the radiopharmaceutical after 30-, 60- and 120-min post-injection. Data here reported demonstrated that the digital autoradiography system is suitable to analyse the biodistribution of an 18F-PSMA inhibitor in both whole small-animal bodies and in single organs. The exposure of both whole mouse bodies and organs on the super resolution screen surface allowed the radioactivity of the PSMA inhibitor distributed in the tissues to be detected and quantified. Data obtained by using a digital autoradiography system were in line with the values detected by the activity calibrator. In addition, the image obtained from the super resolution screen allowed a perfect overlap with the tumour images achieved under the optical microscope. In conclusion, biodistribution studies performed by the autoradiography system allow the microscopical modifications induced by therapeutic radiopharmaceuticals to be studied by comparing the molecular imaging and histopathological data at the sub-cellular level.
format	article
author	Nicoletta Urbano Manuel Scimeca Anna Tolomeo Vincenzo Dimiccoli Elena Bonanno Orazio Schillaci
author_facet	Nicoletta Urbano Manuel Scimeca Anna Tolomeo Vincenzo Dimiccoli Elena Bonanno Orazio Schillaci
author_sort	Nicoletta Urbano
title	Novel Biological and Molecular Characterization in Radiopharmaceutical Preclinical Design
title_short	Novel Biological and Molecular Characterization in Radiopharmaceutical Preclinical Design
title_full	Novel Biological and Molecular Characterization in Radiopharmaceutical Preclinical Design
title_fullStr	Novel Biological and Molecular Characterization in Radiopharmaceutical Preclinical Design
title_full_unstemmed	Novel Biological and Molecular Characterization in Radiopharmaceutical Preclinical Design
title_sort	novel biological and molecular characterization in radiopharmaceutical preclinical design
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4ea74e1fb08b448ca4a70a8a0ddcf136
work_keys_str_mv	AT nicolettaurbano novelbiologicalandmolecularcharacterizationinradiopharmaceuticalpreclinicaldesign AT manuelscimeca novelbiologicalandmolecularcharacterizationinradiopharmaceuticalpreclinicaldesign AT annatolomeo novelbiologicalandmolecularcharacterizationinradiopharmaceuticalpreclinicaldesign AT vincenzodimiccoli novelbiologicalandmolecularcharacterizationinradiopharmaceuticalpreclinicaldesign AT elenabonanno novelbiologicalandmolecularcharacterizationinradiopharmaceuticalpreclinicaldesign AT orazioschillaci novelbiologicalandmolecularcharacterizationinradiopharmaceuticalpreclinicaldesign
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Novel Biological and Molecular Characterization in Radiopharmaceutical Preclinical Design

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