Comprehensive analysis of resistance-nodulation-cell division superfamily (RND) efflux pumps from Serratia marcescens, Db10

Abstract We investigated the role of the resistance-nodulation-cell division superfamily (RND) efflux system on intrinsic multidrug resistance in Serratia marcescens. We identified eight putative RND efflux system genes in the S. marcescens Db10 genome that included the previously characterized syst...

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Autores principales:	Shinsuke Toba, Yusuke Minato, Yuma Kondo, Kanami Hoshikawa, Shu Minagawa, Shiho Komaki, Takanori Kumagai, Yasuyuki Matoba, Daichi Morita, Wakano Ogawa, Naomasa Gotoh, Tomofusa Tsuchiya, Teruo Kuroda
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Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4eae5c160dad49e0ba77c692766c9fd9
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spelling	oai:doaj.org-article:4eae5c160dad49e0ba77c692766c9fd92021-12-02T16:08:52ZComprehensive analysis of resistance-nodulation-cell division superfamily (RND) efflux pumps from Serratia marcescens, Db1010.1038/s41598-019-41237-72045-2322https://doaj.org/article/4eae5c160dad49e0ba77c692766c9fd92019-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-41237-7https://doaj.org/toc/2045-2322Abstract We investigated the role of the resistance-nodulation-cell division superfamily (RND) efflux system on intrinsic multidrug resistance in Serratia marcescens. We identified eight putative RND efflux system genes in the S. marcescens Db10 genome that included the previously characterized systems, sdeXY, sdeAB, and sdeCDE. Six out of the eight genes conferred multidrug resistance on KAM32, a drug hypersensitive strain of Escherichia coli. Five out of the eight genes conferred resistance to benzalkonium, suggesting the importance of RND efflux systems in biocide resistance in S. marcescens. The energy-dependent efflux activities of five of the pumps were examined using a rhodamine 6 G efflux assay. When expressed in the tolC-deficient strain of E. coli, KAM43, none of the genes conferred resistance on E. coli. When hasF, encoding the S. marcescens TolC ortholog, was expressed in KAM43, all of the genes conferred resistance on E. coli, suggesting that HasF is a major outer membrane protein that is used by all RND efflux systems in this organism. We constructed a sdeXY deletion mutant from a derivative strain of the clinically isolated multidrug-resistant S. marcescens strain and found that the sdeXY deletion mutant was sensitive to a broad spectrum of antimicrobial agents.Shinsuke TobaYusuke MinatoYuma KondoKanami HoshikawaShu MinagawaShiho KomakiTakanori KumagaiYasuyuki MatobaDaichi MoritaWakano OgawaNaomasa GotohTomofusa TsuchiyaTeruo KurodaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shinsuke Toba Yusuke Minato Yuma Kondo Kanami Hoshikawa Shu Minagawa Shiho Komaki Takanori Kumagai Yasuyuki Matoba Daichi Morita Wakano Ogawa Naomasa Gotoh Tomofusa Tsuchiya Teruo Kuroda Comprehensive analysis of resistance-nodulation-cell division superfamily (RND) efflux pumps from Serratia marcescens, Db10
description	Abstract We investigated the role of the resistance-nodulation-cell division superfamily (RND) efflux system on intrinsic multidrug resistance in Serratia marcescens. We identified eight putative RND efflux system genes in the S. marcescens Db10 genome that included the previously characterized systems, sdeXY, sdeAB, and sdeCDE. Six out of the eight genes conferred multidrug resistance on KAM32, a drug hypersensitive strain of Escherichia coli. Five out of the eight genes conferred resistance to benzalkonium, suggesting the importance of RND efflux systems in biocide resistance in S. marcescens. The energy-dependent efflux activities of five of the pumps were examined using a rhodamine 6 G efflux assay. When expressed in the tolC-deficient strain of E. coli, KAM43, none of the genes conferred resistance on E. coli. When hasF, encoding the S. marcescens TolC ortholog, was expressed in KAM43, all of the genes conferred resistance on E. coli, suggesting that HasF is a major outer membrane protein that is used by all RND efflux systems in this organism. We constructed a sdeXY deletion mutant from a derivative strain of the clinically isolated multidrug-resistant S. marcescens strain and found that the sdeXY deletion mutant was sensitive to a broad spectrum of antimicrobial agents.
format	article
author	Shinsuke Toba Yusuke Minato Yuma Kondo Kanami Hoshikawa Shu Minagawa Shiho Komaki Takanori Kumagai Yasuyuki Matoba Daichi Morita Wakano Ogawa Naomasa Gotoh Tomofusa Tsuchiya Teruo Kuroda
author_facet	Shinsuke Toba Yusuke Minato Yuma Kondo Kanami Hoshikawa Shu Minagawa Shiho Komaki Takanori Kumagai Yasuyuki Matoba Daichi Morita Wakano Ogawa Naomasa Gotoh Tomofusa Tsuchiya Teruo Kuroda
author_sort	Shinsuke Toba
title	Comprehensive analysis of resistance-nodulation-cell division superfamily (RND) efflux pumps from Serratia marcescens, Db10
title_short	Comprehensive analysis of resistance-nodulation-cell division superfamily (RND) efflux pumps from Serratia marcescens, Db10
title_full	Comprehensive analysis of resistance-nodulation-cell division superfamily (RND) efflux pumps from Serratia marcescens, Db10
title_fullStr	Comprehensive analysis of resistance-nodulation-cell division superfamily (RND) efflux pumps from Serratia marcescens, Db10
title_full_unstemmed	Comprehensive analysis of resistance-nodulation-cell division superfamily (RND) efflux pumps from Serratia marcescens, Db10
title_sort	comprehensive analysis of resistance-nodulation-cell division superfamily (rnd) efflux pumps from serratia marcescens, db10
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/4eae5c160dad49e0ba77c692766c9fd9
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Comprehensive analysis of resistance-nodulation-cell division superfamily (RND) efflux pumps from Serratia marcescens, Db10

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