Schwann cells differentiated from skin-derived precursors provide neuroprotection via autophagy inhibition in a cellular model of Parkinson’s disease

Autophagy has been shown to play an important role in Parkinson’s disease. We hypothesized that skin-derived precursor cells exhibit neuroprotective effects in Parkinson’s disease through affecting autophagy. In this study, 6-hydroxydopamine-damaged SH-SY5Y cells were pretreated with a culture mediu...

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Autores principales:	Jia-Nan Yan, Hai-Ying Zhang, Jun-Rui Li, Ying Chen, Yong-Cheng Jiang, Jia-Bing Shen, Kai-Fu Ke, Xiao-Su Gu
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2022
Materias:	alpha-synuclein; autophagosomes; autophagy; neural regeneration; neuroprotection; parkinson′s disease; pi3k/akt/mtor pathway; skin-derived precursor schwann cells Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/4eb2331ff0b84061875ba3822553891b
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spelling	oai:doaj.org-article:4eb2331ff0b84061875ba3822553891b2021-11-19T12:16:44ZSchwann cells differentiated from skin-derived precursors provide neuroprotection via autophagy inhibition in a cellular model of Parkinson’s disease1673-537410.4103/1673-5374.327353https://doaj.org/article/4eb2331ff0b84061875ba3822553891b2022-01-01T00:00:00Zhttp://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=6;spage=1357;epage=1363;aulast=Yanhttps://doaj.org/toc/1673-5374Autophagy has been shown to play an important role in Parkinson’s disease. We hypothesized that skin-derived precursor cells exhibit neuroprotective effects in Parkinson’s disease through affecting autophagy. In this study, 6-hydroxydopamine-damaged SH-SY5Y cells were pretreated with a culture medium containing skin-derived precursors differentiated into Schwann cells (SKP-SCs). The results showed that the SKP-SC culture medium remarkably enhanced the activity of SH-SY5Y cells damaged by 6-hydroxydopamine, reduced excessive autophagy, increased tyrosine hydroxylase expression, reduced α-synuclein expression, reduced the autophagosome number, and activated the PI3K/AKT/mTOR pathway. Autophagy activator rapamycin inhibited the effects of SKP-SCs, and autophagy inhibitor 3-methyladenine had the opposite effect. These findings confirm that SKP-SCs modulate the PI3K/AKT/mTOR pathway to inhibit autophagy, thereby exhibiting a neuroprotective effect in a cellular model of Parkinson’s disease. This study was approved by the Animal Ethics Committee of Laboratory Animal Center of Nantong University (approval No. S20181009-205) on October 9, 2018.Jia-Nan YanHai-Ying ZhangJun-Rui LiYing ChenYong-Cheng JiangJia-Bing ShenKai-Fu KeXiao-Su GuWolters Kluwer Medknow Publicationsarticlealpha-synuclein; autophagosomes; autophagy; neural regeneration; neuroprotection; parkinson′s disease; pi3k/akt/mtor pathway; skin-derived precursor schwann cellsNeurology. Diseases of the nervous systemRC346-429ENNeural Regeneration Research, Vol 17, Iss 6, Pp 1357-1363 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	alpha-synuclein; autophagosomes; autophagy; neural regeneration; neuroprotection; parkinson′s disease; pi3k/akt/mtor pathway; skin-derived precursor schwann cells Neurology. Diseases of the nervous system RC346-429
spellingShingle	alpha-synuclein; autophagosomes; autophagy; neural regeneration; neuroprotection; parkinson′s disease; pi3k/akt/mtor pathway; skin-derived precursor schwann cells Neurology. Diseases of the nervous system RC346-429 Jia-Nan Yan Hai-Ying Zhang Jun-Rui Li Ying Chen Yong-Cheng Jiang Jia-Bing Shen Kai-Fu Ke Xiao-Su Gu Schwann cells differentiated from skin-derived precursors provide neuroprotection via autophagy inhibition in a cellular model of Parkinson’s disease
description	Autophagy has been shown to play an important role in Parkinson’s disease. We hypothesized that skin-derived precursor cells exhibit neuroprotective effects in Parkinson’s disease through affecting autophagy. In this study, 6-hydroxydopamine-damaged SH-SY5Y cells were pretreated with a culture medium containing skin-derived precursors differentiated into Schwann cells (SKP-SCs). The results showed that the SKP-SC culture medium remarkably enhanced the activity of SH-SY5Y cells damaged by 6-hydroxydopamine, reduced excessive autophagy, increased tyrosine hydroxylase expression, reduced α-synuclein expression, reduced the autophagosome number, and activated the PI3K/AKT/mTOR pathway. Autophagy activator rapamycin inhibited the effects of SKP-SCs, and autophagy inhibitor 3-methyladenine had the opposite effect. These findings confirm that SKP-SCs modulate the PI3K/AKT/mTOR pathway to inhibit autophagy, thereby exhibiting a neuroprotective effect in a cellular model of Parkinson’s disease. This study was approved by the Animal Ethics Committee of Laboratory Animal Center of Nantong University (approval No. S20181009-205) on October 9, 2018.
format	article
author	Jia-Nan Yan Hai-Ying Zhang Jun-Rui Li Ying Chen Yong-Cheng Jiang Jia-Bing Shen Kai-Fu Ke Xiao-Su Gu
author_facet	Jia-Nan Yan Hai-Ying Zhang Jun-Rui Li Ying Chen Yong-Cheng Jiang Jia-Bing Shen Kai-Fu Ke Xiao-Su Gu
author_sort	Jia-Nan Yan
title	Schwann cells differentiated from skin-derived precursors provide neuroprotection via autophagy inhibition in a cellular model of Parkinson’s disease
title_short	Schwann cells differentiated from skin-derived precursors provide neuroprotection via autophagy inhibition in a cellular model of Parkinson’s disease
title_full	Schwann cells differentiated from skin-derived precursors provide neuroprotection via autophagy inhibition in a cellular model of Parkinson’s disease
title_fullStr	Schwann cells differentiated from skin-derived precursors provide neuroprotection via autophagy inhibition in a cellular model of Parkinson’s disease
title_full_unstemmed	Schwann cells differentiated from skin-derived precursors provide neuroprotection via autophagy inhibition in a cellular model of Parkinson’s disease
title_sort	schwann cells differentiated from skin-derived precursors provide neuroprotection via autophagy inhibition in a cellular model of parkinson’s disease
publisher	Wolters Kluwer Medknow Publications
publishDate	2022
url	https://doaj.org/article/4eb2331ff0b84061875ba3822553891b
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Schwann cells differentiated from skin-derived precursors provide neuroprotection via autophagy inhibition in a cellular model of Parkinson’s disease

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