Identification of type III effectors modulating the symbiotic properties of Bradyrhizobium vignae strain ORS3257 with various Vigna species

Abstract The Bradyrhizobium vignae strain ORS3257 is an elite strain recommended for cowpea inoculation in Senegal. This strain was recently shown to establish symbioses on some Aeschynomene species using a cocktail of Type III effectors (T3Es) secreted by the T3SS machinery. In this study, using a...

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Autores principales:	Pongpan Songwattana, Clémence Chaintreuil, Jenjira Wongdee, Albin Teulet, Mamadou Mbaye, Pongdet Piromyou, Djamel Gully, Joel Fardoux, Alexandre Mahougnon Aurel Zoumman, Alicia Camuel, Panlada Tittabutr, Neung Teaumroong, Eric Giraud
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4eb5bd3b6f6447b7813b2e60d2c42f33
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spelling	oai:doaj.org-article:4eb5bd3b6f6447b7813b2e60d2c42f332021-12-02T15:54:10ZIdentification of type III effectors modulating the symbiotic properties of Bradyrhizobium vignae strain ORS3257 with various Vigna species10.1038/s41598-021-84205-w2045-2322https://doaj.org/article/4eb5bd3b6f6447b7813b2e60d2c42f332021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84205-whttps://doaj.org/toc/2045-2322Abstract The Bradyrhizobium vignae strain ORS3257 is an elite strain recommended for cowpea inoculation in Senegal. This strain was recently shown to establish symbioses on some Aeschynomene species using a cocktail of Type III effectors (T3Es) secreted by the T3SS machinery. In this study, using a collection of mutants in different T3Es genes, we sought to identify the effectors that modulate the symbiotic properties of ORS3257 in three Vigna species (V. unguiculata, V. radiata and V. mungo). While the T3SS had a positive impact on the symbiotic efficiency of the strain in V. unguiculata and V. mungo, it blocked symbiosis with V. radiata. The combination of effectors promoting nodulation in V. unguiculata and V. mungo differed, in both cases, NopT and NopAB were involved, suggesting they are key determinants for nodulation, and to a lesser extent, NopM1 and NopP1, which are additionally required for optimal symbiosis with V. mungo. In contrast, only one effector, NopP2, was identified as the cause of the incompatibility between ORS3257 and V. radiata. The identification of key effectors which promote symbiotic efficiency or render the interaction incompatible is important for the development of inoculation strategies to improve the growth of Vigna species cultivated in Africa and Asia.Pongpan SongwattanaClémence ChaintreuilJenjira WongdeeAlbin TeuletMamadou MbayePongdet PiromyouDjamel GullyJoel FardouxAlexandre Mahougnon Aurel ZoummanAlicia CamuelPanlada TittabutrNeung TeaumroongEric GiraudNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pongpan Songwattana Clémence Chaintreuil Jenjira Wongdee Albin Teulet Mamadou Mbaye Pongdet Piromyou Djamel Gully Joel Fardoux Alexandre Mahougnon Aurel Zoumman Alicia Camuel Panlada Tittabutr Neung Teaumroong Eric Giraud Identification of type III effectors modulating the symbiotic properties of Bradyrhizobium vignae strain ORS3257 with various Vigna species
description	Abstract The Bradyrhizobium vignae strain ORS3257 is an elite strain recommended for cowpea inoculation in Senegal. This strain was recently shown to establish symbioses on some Aeschynomene species using a cocktail of Type III effectors (T3Es) secreted by the T3SS machinery. In this study, using a collection of mutants in different T3Es genes, we sought to identify the effectors that modulate the symbiotic properties of ORS3257 in three Vigna species (V. unguiculata, V. radiata and V. mungo). While the T3SS had a positive impact on the symbiotic efficiency of the strain in V. unguiculata and V. mungo, it blocked symbiosis with V. radiata. The combination of effectors promoting nodulation in V. unguiculata and V. mungo differed, in both cases, NopT and NopAB were involved, suggesting they are key determinants for nodulation, and to a lesser extent, NopM1 and NopP1, which are additionally required for optimal symbiosis with V. mungo. In contrast, only one effector, NopP2, was identified as the cause of the incompatibility between ORS3257 and V. radiata. The identification of key effectors which promote symbiotic efficiency or render the interaction incompatible is important for the development of inoculation strategies to improve the growth of Vigna species cultivated in Africa and Asia.
format	article
author	Pongpan Songwattana Clémence Chaintreuil Jenjira Wongdee Albin Teulet Mamadou Mbaye Pongdet Piromyou Djamel Gully Joel Fardoux Alexandre Mahougnon Aurel Zoumman Alicia Camuel Panlada Tittabutr Neung Teaumroong Eric Giraud
author_facet	Pongpan Songwattana Clémence Chaintreuil Jenjira Wongdee Albin Teulet Mamadou Mbaye Pongdet Piromyou Djamel Gully Joel Fardoux Alexandre Mahougnon Aurel Zoumman Alicia Camuel Panlada Tittabutr Neung Teaumroong Eric Giraud
author_sort	Pongpan Songwattana
title	Identification of type III effectors modulating the symbiotic properties of Bradyrhizobium vignae strain ORS3257 with various Vigna species
title_short	Identification of type III effectors modulating the symbiotic properties of Bradyrhizobium vignae strain ORS3257 with various Vigna species
title_full	Identification of type III effectors modulating the symbiotic properties of Bradyrhizobium vignae strain ORS3257 with various Vigna species
title_fullStr	Identification of type III effectors modulating the symbiotic properties of Bradyrhizobium vignae strain ORS3257 with various Vigna species
title_full_unstemmed	Identification of type III effectors modulating the symbiotic properties of Bradyrhizobium vignae strain ORS3257 with various Vigna species
title_sort	identification of type iii effectors modulating the symbiotic properties of bradyrhizobium vignae strain ors3257 with various vigna species
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4eb5bd3b6f6447b7813b2e60d2c42f33
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Identification of type III effectors modulating the symbiotic properties of Bradyrhizobium vignae strain ORS3257 with various Vigna species

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