Evaluation of the Effects of Library Preparation Procedure and Sample Characteristics on the Accuracy of Metagenomic Profiles

Metagenomic library preparation methods and sequencing technologies continue to advance rapidly, allowing researchers to characterize microbial communities in previously underexplored environmental samples and systems. However, widely accepted standardized library preparation methods can be cost-pr...

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Autores principales: Christopher A. Gaulke, Emily R. Schmeltzer, Mark Dasenko, Brett M. Tyler, Rebecca Vega Thurber, Thomas J. Sharpton
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2021
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Acceso en línea:https://doaj.org/article/4eb7dfba249d43d592016040f0da5cf0
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spelling oai:doaj.org-article:4eb7dfba249d43d592016040f0da5cf02021-12-02T16:51:26ZEvaluation of the Effects of Library Preparation Procedure and Sample Characteristics on the Accuracy of Metagenomic Profiles2379-507710.1128/mSystems.00440-21https://doaj.org/article/4eb7dfba249d43d592016040f0da5cf02021-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00440-21https://doaj.org/toc/2379-5077 Metagenomic library preparation methods and sequencing technologies continue to advance rapidly, allowing researchers to characterize microbial communities in previously underexplored environmental samples and systems. However, widely accepted standardized library preparation methods can be cost-prohibitive.Christopher A. GaulkeEmily R. SchmeltzerMark DasenkoBrett M. TylerRebecca Vega ThurberThomas J. SharptonAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmSystems, Vol 6, Iss 5 (2021)
institution DOAJ
collection DOAJ
language EN
topic Microbiology
QR1-502
spellingShingle Microbiology
QR1-502
Christopher A. Gaulke
Emily R. Schmeltzer
Mark Dasenko
Brett M. Tyler
Rebecca Vega Thurber
Thomas J. Sharpton
Evaluation of the Effects of Library Preparation Procedure and Sample Characteristics on the Accuracy of Metagenomic Profiles
description Metagenomic library preparation methods and sequencing technologies continue to advance rapidly, allowing researchers to characterize microbial communities in previously underexplored environmental samples and systems. However, widely accepted standardized library preparation methods can be cost-prohibitive.
format article
author Christopher A. Gaulke
Emily R. Schmeltzer
Mark Dasenko
Brett M. Tyler
Rebecca Vega Thurber
Thomas J. Sharpton
author_facet Christopher A. Gaulke
Emily R. Schmeltzer
Mark Dasenko
Brett M. Tyler
Rebecca Vega Thurber
Thomas J. Sharpton
author_sort Christopher A. Gaulke
title Evaluation of the Effects of Library Preparation Procedure and Sample Characteristics on the Accuracy of Metagenomic Profiles
title_short Evaluation of the Effects of Library Preparation Procedure and Sample Characteristics on the Accuracy of Metagenomic Profiles
title_full Evaluation of the Effects of Library Preparation Procedure and Sample Characteristics on the Accuracy of Metagenomic Profiles
title_fullStr Evaluation of the Effects of Library Preparation Procedure and Sample Characteristics on the Accuracy of Metagenomic Profiles
title_full_unstemmed Evaluation of the Effects of Library Preparation Procedure and Sample Characteristics on the Accuracy of Metagenomic Profiles
title_sort evaluation of the effects of library preparation procedure and sample characteristics on the accuracy of metagenomic profiles
publisher American Society for Microbiology
publishDate 2021
url https://doaj.org/article/4eb7dfba249d43d592016040f0da5cf0
work_keys_str_mv AT christopheragaulke evaluationoftheeffectsoflibrarypreparationprocedureandsamplecharacteristicsontheaccuracyofmetagenomicprofiles
AT emilyrschmeltzer evaluationoftheeffectsoflibrarypreparationprocedureandsamplecharacteristicsontheaccuracyofmetagenomicprofiles
AT markdasenko evaluationoftheeffectsoflibrarypreparationprocedureandsamplecharacteristicsontheaccuracyofmetagenomicprofiles
AT brettmtyler evaluationoftheeffectsoflibrarypreparationprocedureandsamplecharacteristicsontheaccuracyofmetagenomicprofiles
AT rebeccavegathurber evaluationoftheeffectsoflibrarypreparationprocedureandsamplecharacteristicsontheaccuracyofmetagenomicprofiles
AT thomasjsharpton evaluationoftheeffectsoflibrarypreparationprocedureandsamplecharacteristicsontheaccuracyofmetagenomicprofiles
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