Negatively charged silver nanoparticles with potent antibacterial activity and reduced toxicity for pharmaceutical preparations

Lucia Salvioni,1 Elisabetta Galbiati,1 Veronica Collico,1 Giulia Alessio,1 Svetlana Avvakumova,1 Fabio Corsi,2,3 Paolo Tortora,1 Davide Prosperi,1 Miriam Colombo1 1Nanobiolab, Department of Biotechnology and Bioscience, University of Milano-Bicocca, 2Biological and Clinical Science Department, Univ...

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Autores principales:	Salvioni L, Galbiati E, Collico V, Alessio G, Avvakumova S, Corsi F, Tortora P, Prosperi D, Colombo M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	silver nanoparticles antibacterial activity long term effect nanoparticle toxicity phase transfer Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4ebc6db0565846f081dabdec94cdf3de
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spelling	oai:doaj.org-article:4ebc6db0565846f081dabdec94cdf3de2021-12-02T01:50:19ZNegatively charged silver nanoparticles with potent antibacterial activity and reduced toxicity for pharmaceutical preparations1178-2013https://doaj.org/article/4ebc6db0565846f081dabdec94cdf3de2017-03-01T00:00:00Zhttps://www.dovepress.com/negatively-charged-silver-nanoparticles-with-potent-antibacterial-acti-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Lucia Salvioni,1 Elisabetta Galbiati,1 Veronica Collico,1 Giulia Alessio,1 Svetlana Avvakumova,1 Fabio Corsi,2,3 Paolo Tortora,1 Davide Prosperi,1 Miriam Colombo1 1Nanobiolab, Department of Biotechnology and Bioscience, University of Milano-Bicocca, 2Biological and Clinical Science Department, University of Milan, Milano, 3Surgery Department, Breast Unit, IRCCS S Maugeri Foundation, Pavia, Italy Background: The discovery of new solutions with antibacterial activity as efficient and safe alternatives to common preservatives (such as parabens) and to combat emerging infections and drug-resistant bacterial pathogens is highly expected in cosmetics and pharmaceutics. Colloidal silver nanoparticles (NPs) are attracting interest as novel effective antimicrobial agents for the prevention of several infectious diseases.Methods: Water-soluble, negatively charged silver nanoparticles (AgNPs) were synthesized by reduction with citric and tannic acid and characterized by transmission electron microscopy, dynamic light scattering, zeta potential, differential centrifuge sedimentation, and ultraviolet–visible spectroscopy. AgNPs were tested with model Gram-negative and Gram-positive bacteria in comparison to two different kinds of commercially available AgNPs.Results: In this work, AgNPs with higher antibacterial activity compared to the commercially available colloidal silver solutions were prepared and investigated. Bacteria were plated and the antibacterial activity was tested at the same concentration of silver ions in all samples. The AgNPs did not show any significant reduction in the antibacterial activity for an acceptable time period. In addition, AgNPs were transferred to organic phase and retained their antibacterial efficacy in both aqueous and nonaqueous media and exhibited no toxicity in eukaryotic cells.Conclusion: We developed AgNPs with a 20 nm diameter and negative zeta potential with powerful antibacterial activity and low toxicity compared to currently available colloidal silver, suitable for cosmetic preservatives and pharmaceutical preparations administrable to humans and/or animals as needed. Keywords: silver nanoparticles, antibacterial activity, long-term effect, nanoparticle toxicity, phase transferSalvioni LGalbiati ECollico VAlessio GAvvakumova SCorsi FTortora PProsperi DColombo MDove Medical Pressarticlesilver nanoparticlesantibacterial activitylong term effectnanoparticle toxicityphase transferMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 2517-2530 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	silver nanoparticles antibacterial activity long term effect nanoparticle toxicity phase transfer Medicine (General) R5-920
spellingShingle	silver nanoparticles antibacterial activity long term effect nanoparticle toxicity phase transfer Medicine (General) R5-920 Salvioni L Galbiati E Collico V Alessio G Avvakumova S Corsi F Tortora P Prosperi D Colombo M Negatively charged silver nanoparticles with potent antibacterial activity and reduced toxicity for pharmaceutical preparations
description	Lucia Salvioni,1 Elisabetta Galbiati,1 Veronica Collico,1 Giulia Alessio,1 Svetlana Avvakumova,1 Fabio Corsi,2,3 Paolo Tortora,1 Davide Prosperi,1 Miriam Colombo1 1Nanobiolab, Department of Biotechnology and Bioscience, University of Milano-Bicocca, 2Biological and Clinical Science Department, University of Milan, Milano, 3Surgery Department, Breast Unit, IRCCS S Maugeri Foundation, Pavia, Italy Background: The discovery of new solutions with antibacterial activity as efficient and safe alternatives to common preservatives (such as parabens) and to combat emerging infections and drug-resistant bacterial pathogens is highly expected in cosmetics and pharmaceutics. Colloidal silver nanoparticles (NPs) are attracting interest as novel effective antimicrobial agents for the prevention of several infectious diseases.Methods: Water-soluble, negatively charged silver nanoparticles (AgNPs) were synthesized by reduction with citric and tannic acid and characterized by transmission electron microscopy, dynamic light scattering, zeta potential, differential centrifuge sedimentation, and ultraviolet–visible spectroscopy. AgNPs were tested with model Gram-negative and Gram-positive bacteria in comparison to two different kinds of commercially available AgNPs.Results: In this work, AgNPs with higher antibacterial activity compared to the commercially available colloidal silver solutions were prepared and investigated. Bacteria were plated and the antibacterial activity was tested at the same concentration of silver ions in all samples. The AgNPs did not show any significant reduction in the antibacterial activity for an acceptable time period. In addition, AgNPs were transferred to organic phase and retained their antibacterial efficacy in both aqueous and nonaqueous media and exhibited no toxicity in eukaryotic cells.Conclusion: We developed AgNPs with a 20 nm diameter and negative zeta potential with powerful antibacterial activity and low toxicity compared to currently available colloidal silver, suitable for cosmetic preservatives and pharmaceutical preparations administrable to humans and/or animals as needed. Keywords: silver nanoparticles, antibacterial activity, long-term effect, nanoparticle toxicity, phase transfer
format	article
author	Salvioni L Galbiati E Collico V Alessio G Avvakumova S Corsi F Tortora P Prosperi D Colombo M
author_facet	Salvioni L Galbiati E Collico V Alessio G Avvakumova S Corsi F Tortora P Prosperi D Colombo M
author_sort	Salvioni L
title	Negatively charged silver nanoparticles with potent antibacterial activity and reduced toxicity for pharmaceutical preparations
title_short	Negatively charged silver nanoparticles with potent antibacterial activity and reduced toxicity for pharmaceutical preparations
title_full	Negatively charged silver nanoparticles with potent antibacterial activity and reduced toxicity for pharmaceutical preparations
title_fullStr	Negatively charged silver nanoparticles with potent antibacterial activity and reduced toxicity for pharmaceutical preparations
title_full_unstemmed	Negatively charged silver nanoparticles with potent antibacterial activity and reduced toxicity for pharmaceutical preparations
title_sort	negatively charged silver nanoparticles with potent antibacterial activity and reduced toxicity for pharmaceutical preparations
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/4ebc6db0565846f081dabdec94cdf3de
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_version_	1718402835027066880

Negatively charged silver nanoparticles with potent antibacterial activity and reduced toxicity for pharmaceutical preparations

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