The Potent and Broadly Neutralizing Human Dengue Virus-Specific Monoclonal Antibody 1C19 Reveals a Unique Cross-Reactive Epitope on the bc Loop of Domain II of the Envelope Protein

ABSTRACT Following natural dengue virus (DENV) infection, humans produce some antibodies that recognize only the serotype of infection (type specific) and others that cross-react with all four serotypes (cross-reactive). Recent studies with human antibodies indicate that type-specific antibodies at...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Scott A. Smith, A. Ruklanthi de Alwis, Nurgun Kose, Eva Harris, Kristie D. Ibarra, Kristen M. Kahle, Jennifer M. Pfaff, Xiaoxiao Xiang, Benjamin J. Doranz, Aravinda M. de Silva, S. Kyle Austin, Soila Sukupolvi-Petty, Michael S. Diamond, James E. Crowe
Formato: article
Lenguaje:EN
Publicado: American Society for Microbiology 2013
Materias:
Acceso en línea:https://doaj.org/article/4ebe8688b7984900b3d2b984de558908
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:4ebe8688b7984900b3d2b984de558908
record_format dspace
spelling oai:doaj.org-article:4ebe8688b7984900b3d2b984de5589082021-11-15T15:42:31ZThe Potent and Broadly Neutralizing Human Dengue Virus-Specific Monoclonal Antibody 1C19 Reveals a Unique Cross-Reactive Epitope on the bc Loop of Domain II of the Envelope Protein10.1128/mBio.00873-132150-7511https://doaj.org/article/4ebe8688b7984900b3d2b984de5589082013-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00873-13https://doaj.org/toc/2150-7511ABSTRACT Following natural dengue virus (DENV) infection, humans produce some antibodies that recognize only the serotype of infection (type specific) and others that cross-react with all four serotypes (cross-reactive). Recent studies with human antibodies indicate that type-specific antibodies at high concentrations are often strongly neutralizing in vitro and protective in animal models. In general, cross-reactive antibodies are poorly neutralizing and can enhance the ability of DENV to infect Fc receptor-bearing cells under some conditions. Type-specific antibodies at low concentrations also may enhance infection. There is an urgent need to determine whether there are conserved antigenic sites that can be recognized by cross-reactive potently neutralizing antibodies. Here, we describe the isolation of a large panel of naturally occurring human monoclonal antibodies (MAbs) directed to the DENV domain II fusion loop (FL) envelope protein region from subjects following vaccination or natural infection. Most of the FL-specific antibodies exhibited a conventional phenotype, characterized by low-potency neutralizing function and antibody-dependent enhancing activity. One clone, however, recognized the bc loop of domain II adjacent to the FL and exhibited a unique phenotype of ultrahigh potency, neutralizing all four serotypes better than any other previously described MAb recognizing this region. This antibody not only neutralized DENV effectively but also competed for binding against the more prevalent poor-quality antibodies whose binding was focused on the FL. The 1C19 human antibody could be a promising component of a preventative or therapeutic intervention. Furthermore, the unique epitope revealed by 1C19 suggests a focus for rational vaccine design based on novel immunogens presenting cross-reactive neutralizing determinants. IMPORTANCE With no effective vaccine available, the incidence of dengue virus (DENV) infections worldwide continues to rise, with more than 390 million infections estimated to occur each year. Due to the unique roles that antibodies are postulated to play in the pathogenesis of DENV infection and disease, there is consensus that a successful DENV vaccine must protect against all four serotypes. If conserved epitopes recognized by naturally occurring potently cross-neutralizing human antibodies could be identified, monovalent subunit vaccine preparations might be developed. We characterized 30 DENV cross-neutralizing human monoclonal antibodies (MAbs) and identified one (1C19) that recognized a novel conserved site, known as the bc loop. This antibody has several desirable features, as it neutralizes DENV effectively and competes for binding against the more common low-potency fusion loop (FL) antibodies, which are believed to contribute to antibody-mediated disease. To our knowledge, this is the first description of a potent serotype cross-neutralizing human antibody to DENV.Scott A. SmithA. Ruklanthi de AlwisNurgun KoseEva HarrisKristie D. IbarraKristen M. KahleJennifer M. PfaffXiaoxiao XiangBenjamin J. DoranzAravinda M. de SilvaS. Kyle AustinSoila Sukupolvi-PettyMichael S. DiamondJames E. CroweAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 4, Iss 6 (2013)
institution DOAJ
collection DOAJ
language EN
topic Microbiology
QR1-502
spellingShingle Microbiology
QR1-502
Scott A. Smith
A. Ruklanthi de Alwis
Nurgun Kose
Eva Harris
Kristie D. Ibarra
Kristen M. Kahle
Jennifer M. Pfaff
Xiaoxiao Xiang
Benjamin J. Doranz
Aravinda M. de Silva
S. Kyle Austin
Soila Sukupolvi-Petty
Michael S. Diamond
James E. Crowe
The Potent and Broadly Neutralizing Human Dengue Virus-Specific Monoclonal Antibody 1C19 Reveals a Unique Cross-Reactive Epitope on the bc Loop of Domain II of the Envelope Protein
description ABSTRACT Following natural dengue virus (DENV) infection, humans produce some antibodies that recognize only the serotype of infection (type specific) and others that cross-react with all four serotypes (cross-reactive). Recent studies with human antibodies indicate that type-specific antibodies at high concentrations are often strongly neutralizing in vitro and protective in animal models. In general, cross-reactive antibodies are poorly neutralizing and can enhance the ability of DENV to infect Fc receptor-bearing cells under some conditions. Type-specific antibodies at low concentrations also may enhance infection. There is an urgent need to determine whether there are conserved antigenic sites that can be recognized by cross-reactive potently neutralizing antibodies. Here, we describe the isolation of a large panel of naturally occurring human monoclonal antibodies (MAbs) directed to the DENV domain II fusion loop (FL) envelope protein region from subjects following vaccination or natural infection. Most of the FL-specific antibodies exhibited a conventional phenotype, characterized by low-potency neutralizing function and antibody-dependent enhancing activity. One clone, however, recognized the bc loop of domain II adjacent to the FL and exhibited a unique phenotype of ultrahigh potency, neutralizing all four serotypes better than any other previously described MAb recognizing this region. This antibody not only neutralized DENV effectively but also competed for binding against the more prevalent poor-quality antibodies whose binding was focused on the FL. The 1C19 human antibody could be a promising component of a preventative or therapeutic intervention. Furthermore, the unique epitope revealed by 1C19 suggests a focus for rational vaccine design based on novel immunogens presenting cross-reactive neutralizing determinants. IMPORTANCE With no effective vaccine available, the incidence of dengue virus (DENV) infections worldwide continues to rise, with more than 390 million infections estimated to occur each year. Due to the unique roles that antibodies are postulated to play in the pathogenesis of DENV infection and disease, there is consensus that a successful DENV vaccine must protect against all four serotypes. If conserved epitopes recognized by naturally occurring potently cross-neutralizing human antibodies could be identified, monovalent subunit vaccine preparations might be developed. We characterized 30 DENV cross-neutralizing human monoclonal antibodies (MAbs) and identified one (1C19) that recognized a novel conserved site, known as the bc loop. This antibody has several desirable features, as it neutralizes DENV effectively and competes for binding against the more common low-potency fusion loop (FL) antibodies, which are believed to contribute to antibody-mediated disease. To our knowledge, this is the first description of a potent serotype cross-neutralizing human antibody to DENV.
format article
author Scott A. Smith
A. Ruklanthi de Alwis
Nurgun Kose
Eva Harris
Kristie D. Ibarra
Kristen M. Kahle
Jennifer M. Pfaff
Xiaoxiao Xiang
Benjamin J. Doranz
Aravinda M. de Silva
S. Kyle Austin
Soila Sukupolvi-Petty
Michael S. Diamond
James E. Crowe
author_facet Scott A. Smith
A. Ruklanthi de Alwis
Nurgun Kose
Eva Harris
Kristie D. Ibarra
Kristen M. Kahle
Jennifer M. Pfaff
Xiaoxiao Xiang
Benjamin J. Doranz
Aravinda M. de Silva
S. Kyle Austin
Soila Sukupolvi-Petty
Michael S. Diamond
James E. Crowe
author_sort Scott A. Smith
title The Potent and Broadly Neutralizing Human Dengue Virus-Specific Monoclonal Antibody 1C19 Reveals a Unique Cross-Reactive Epitope on the bc Loop of Domain II of the Envelope Protein
title_short The Potent and Broadly Neutralizing Human Dengue Virus-Specific Monoclonal Antibody 1C19 Reveals a Unique Cross-Reactive Epitope on the bc Loop of Domain II of the Envelope Protein
title_full The Potent and Broadly Neutralizing Human Dengue Virus-Specific Monoclonal Antibody 1C19 Reveals a Unique Cross-Reactive Epitope on the bc Loop of Domain II of the Envelope Protein
title_fullStr The Potent and Broadly Neutralizing Human Dengue Virus-Specific Monoclonal Antibody 1C19 Reveals a Unique Cross-Reactive Epitope on the bc Loop of Domain II of the Envelope Protein
title_full_unstemmed The Potent and Broadly Neutralizing Human Dengue Virus-Specific Monoclonal Antibody 1C19 Reveals a Unique Cross-Reactive Epitope on the bc Loop of Domain II of the Envelope Protein
title_sort potent and broadly neutralizing human dengue virus-specific monoclonal antibody 1c19 reveals a unique cross-reactive epitope on the bc loop of domain ii of the envelope protein
publisher American Society for Microbiology
publishDate 2013
url https://doaj.org/article/4ebe8688b7984900b3d2b984de558908
work_keys_str_mv AT scottasmith thepotentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT aruklanthidealwis thepotentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT nurgunkose thepotentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT evaharris thepotentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT kristiedibarra thepotentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT kristenmkahle thepotentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT jennifermpfaff thepotentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT xiaoxiaoxiang thepotentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT benjaminjdoranz thepotentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT aravindamdesilva thepotentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT skyleaustin thepotentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT soilasukupolvipetty thepotentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT michaelsdiamond thepotentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT jamesecrowe thepotentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT scottasmith potentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT aruklanthidealwis potentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT nurgunkose potentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT evaharris potentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT kristiedibarra potentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT kristenmkahle potentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT jennifermpfaff potentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT xiaoxiaoxiang potentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT benjaminjdoranz potentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT aravindamdesilva potentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT skyleaustin potentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT soilasukupolvipetty potentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT michaelsdiamond potentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
AT jamesecrowe potentandbroadlyneutralizinghumandenguevirusspecificmonoclonalantibody1c19revealsauniquecrossreactiveepitopeonthebcloopofdomainiioftheenvelopeprotein
_version_ 1718427581873651712