Genome-Wide Analysis of CCT Transcript Factors to Identify Genes Contributing to Photoperiodic Flowering in Oryza rufipogon

Photoperiod sensitivity is a dominant determinant for the phase transition in cereal crops. CCT (CONSTANS, CO-like, and TOC1) transcription factors (TFs) are involved in many physiological functions including the regulation of the photoperiodic flowering. However, the functional roles of CCT TFs hav...

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Autores principales:	Xin Peng, Win Tun, Shuang-feng Dai, Jia-yue Li, Qun-jie Zhang, Guo-ying Yin, Jinmi Yoon, Lae-hyeon Cho, Gynheung An, Li-zhi Gao
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Oryza rufipogon rice CCT genes genomic synteny expression profiles photoperiodic flowering regulation Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/4ec033f31c514b90889b438790827bf1
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spelling	oai:doaj.org-article:4ec033f31c514b90889b438790827bf12021-11-08T07:18:35ZGenome-Wide Analysis of CCT Transcript Factors to Identify Genes Contributing to Photoperiodic Flowering in Oryza rufipogon1664-462X10.3389/fpls.2021.736419https://doaj.org/article/4ec033f31c514b90889b438790827bf12021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.736419/fullhttps://doaj.org/toc/1664-462XPhotoperiod sensitivity is a dominant determinant for the phase transition in cereal crops. CCT (CONSTANS, CO-like, and TOC1) transcription factors (TFs) are involved in many physiological functions including the regulation of the photoperiodic flowering. However, the functional roles of CCT TFs have not been elucidated in the wild progenitors of crops. In this study, we identified 41 CCT TFs, including 19 CMF, 17 COL, and five PRR TFs in Oryza rufipogon, the presumed wild ancestor of Asian cultivated rice. There are thirty-eight orthologous CCT genes in Oryza sativa, of which ten pairs of duplicated CCT TFs are shared with O. rufipogon. We investigated daily expression patterns, showing that 36 OrCCT genes exhibited circadian rhythmic expression. A total of thirteen OrCCT genes were identified as putative flowering suppressors in O. rufipogon based on rhythmic and developmental expression patterns and transgenic phenotypes. We propose that OrCCT08, OrCCT24, and OrCCT26 are the strong functional alleles of rice DTH2, Ghd7, and OsPRR37, respectively. The SD treatment at 80 DAG stimulated flowering of the LD-grown O. rufipogon plants. Our results further showed that the nine OrCCT genes were significantly downregulated under the treatment. Our findings would provide valuable information for the construction of photoperiodic flowering regulatory network and functional characterization of the CCT TFs in both O. rufipogon and O. sativa.Xin PengXin PengWin TunShuang-feng DaiJia-yue LiQun-jie ZhangGuo-ying YinJinmi YoonLae-hyeon ChoLae-hyeon ChoGynheung AnLi-zhi GaoFrontiers Media S.A.articleOryza rufipogonriceCCT genesgenomic syntenyexpression profilesphotoperiodic flowering regulationPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Oryza rufipogon rice CCT genes genomic synteny expression profiles photoperiodic flowering regulation Plant culture SB1-1110
spellingShingle	Oryza rufipogon rice CCT genes genomic synteny expression profiles photoperiodic flowering regulation Plant culture SB1-1110 Xin Peng Xin Peng Win Tun Shuang-feng Dai Jia-yue Li Qun-jie Zhang Guo-ying Yin Jinmi Yoon Lae-hyeon Cho Lae-hyeon Cho Gynheung An Li-zhi Gao Genome-Wide Analysis of CCT Transcript Factors to Identify Genes Contributing to Photoperiodic Flowering in Oryza rufipogon
description	Photoperiod sensitivity is a dominant determinant for the phase transition in cereal crops. CCT (CONSTANS, CO-like, and TOC1) transcription factors (TFs) are involved in many physiological functions including the regulation of the photoperiodic flowering. However, the functional roles of CCT TFs have not been elucidated in the wild progenitors of crops. In this study, we identified 41 CCT TFs, including 19 CMF, 17 COL, and five PRR TFs in Oryza rufipogon, the presumed wild ancestor of Asian cultivated rice. There are thirty-eight orthologous CCT genes in Oryza sativa, of which ten pairs of duplicated CCT TFs are shared with O. rufipogon. We investigated daily expression patterns, showing that 36 OrCCT genes exhibited circadian rhythmic expression. A total of thirteen OrCCT genes were identified as putative flowering suppressors in O. rufipogon based on rhythmic and developmental expression patterns and transgenic phenotypes. We propose that OrCCT08, OrCCT24, and OrCCT26 are the strong functional alleles of rice DTH2, Ghd7, and OsPRR37, respectively. The SD treatment at 80 DAG stimulated flowering of the LD-grown O. rufipogon plants. Our results further showed that the nine OrCCT genes were significantly downregulated under the treatment. Our findings would provide valuable information for the construction of photoperiodic flowering regulatory network and functional characterization of the CCT TFs in both O. rufipogon and O. sativa.
format	article
author	Xin Peng Xin Peng Win Tun Shuang-feng Dai Jia-yue Li Qun-jie Zhang Guo-ying Yin Jinmi Yoon Lae-hyeon Cho Lae-hyeon Cho Gynheung An Li-zhi Gao
author_facet	Xin Peng Xin Peng Win Tun Shuang-feng Dai Jia-yue Li Qun-jie Zhang Guo-ying Yin Jinmi Yoon Lae-hyeon Cho Lae-hyeon Cho Gynheung An Li-zhi Gao
author_sort	Xin Peng
title	Genome-Wide Analysis of CCT Transcript Factors to Identify Genes Contributing to Photoperiodic Flowering in Oryza rufipogon
title_short	Genome-Wide Analysis of CCT Transcript Factors to Identify Genes Contributing to Photoperiodic Flowering in Oryza rufipogon
title_full	Genome-Wide Analysis of CCT Transcript Factors to Identify Genes Contributing to Photoperiodic Flowering in Oryza rufipogon
title_fullStr	Genome-Wide Analysis of CCT Transcript Factors to Identify Genes Contributing to Photoperiodic Flowering in Oryza rufipogon
title_full_unstemmed	Genome-Wide Analysis of CCT Transcript Factors to Identify Genes Contributing to Photoperiodic Flowering in Oryza rufipogon
title_sort	genome-wide analysis of cct transcript factors to identify genes contributing to photoperiodic flowering in oryza rufipogon
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/4ec033f31c514b90889b438790827bf1
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Genome-Wide Analysis of CCT Transcript Factors to Identify Genes Contributing to Photoperiodic Flowering in Oryza rufipogon

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