Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius
Abstract The bed bug Cimex lectularius is a blood-feeding re-emerging annoyance pest insect that has the ability to transmit Trypanosoma cruzi under experimental laboratory conditions. Aquaporins (AQPs) are water channel proteins that are essential in biological organisms. C. lectularius are constan...
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2017
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oai:doaj.org-article:4ec7d6d03f9b42818a591ff75f9b1acb2021-12-02T16:06:33ZFunctional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius10.1038/s41598-017-03157-22045-2322https://doaj.org/article/4ec7d6d03f9b42818a591ff75f9b1acb2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03157-2https://doaj.org/toc/2045-2322Abstract The bed bug Cimex lectularius is a blood-feeding re-emerging annoyance pest insect that has the ability to transmit Trypanosoma cruzi under experimental laboratory conditions. Aquaporins (AQPs) are water channel proteins that are essential in biological organisms. C. lectularius are constantly exposed to water-related stress, suggesting that AQPs may offer novel control avenues. We identified and cloned four AQPs from C. lectularius, assessed tissue and lifestage-specific expression, and characterized biochemical functions in vitro and in vivo. We identified an efficient water-specific AQP (ClAQP1), two aquaglyceroporins (ClGlp1 and ClGlp2) and a homolog of Drosophila melanogaster big brain (ClBib). ClGlp1 was only functional when co-expressed with the water-specific AQP. Simultaneous RNAi gene silencing of ClAQP1 and ClGlp1 significantly reduced water and urea excretion post blood feeding. The Bib homologue was enriched in embryos, exclusively expressed in ovaries, and when silenced, dramatically increased bug fecundity. Our data demonstrate that AQPs have critical roles in excretion, water homeostasis and reproduction in C. lectularius, and could be potential targets for control in this notorious pest.Hitoshi TsujimotoJoyce M. SakamotoJason L. RasgonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) |
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Medicine R Science Q Hitoshi Tsujimoto Joyce M. Sakamoto Jason L. Rasgon Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius |
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Abstract The bed bug Cimex lectularius is a blood-feeding re-emerging annoyance pest insect that has the ability to transmit Trypanosoma cruzi under experimental laboratory conditions. Aquaporins (AQPs) are water channel proteins that are essential in biological organisms. C. lectularius are constantly exposed to water-related stress, suggesting that AQPs may offer novel control avenues. We identified and cloned four AQPs from C. lectularius, assessed tissue and lifestage-specific expression, and characterized biochemical functions in vitro and in vivo. We identified an efficient water-specific AQP (ClAQP1), two aquaglyceroporins (ClGlp1 and ClGlp2) and a homolog of Drosophila melanogaster big brain (ClBib). ClGlp1 was only functional when co-expressed with the water-specific AQP. Simultaneous RNAi gene silencing of ClAQP1 and ClGlp1 significantly reduced water and urea excretion post blood feeding. The Bib homologue was enriched in embryos, exclusively expressed in ovaries, and when silenced, dramatically increased bug fecundity. Our data demonstrate that AQPs have critical roles in excretion, water homeostasis and reproduction in C. lectularius, and could be potential targets for control in this notorious pest. |
format |
article |
author |
Hitoshi Tsujimoto Joyce M. Sakamoto Jason L. Rasgon |
author_facet |
Hitoshi Tsujimoto Joyce M. Sakamoto Jason L. Rasgon |
author_sort |
Hitoshi Tsujimoto |
title |
Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius |
title_short |
Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius |
title_full |
Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius |
title_fullStr |
Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius |
title_full_unstemmed |
Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius |
title_sort |
functional characterization of aquaporin-like genes in the human bed bug cimex lectularius |
publisher |
Nature Portfolio |
publishDate |
2017 |
url |
https://doaj.org/article/4ec7d6d03f9b42818a591ff75f9b1acb |
work_keys_str_mv |
AT hitoshitsujimoto functionalcharacterizationofaquaporinlikegenesinthehumanbedbugcimexlectularius AT joycemsakamoto functionalcharacterizationofaquaporinlikegenesinthehumanbedbugcimexlectularius AT jasonlrasgon functionalcharacterizationofaquaporinlikegenesinthehumanbedbugcimexlectularius |
_version_ |
1718384959017713664 |