Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius

Abstract The bed bug Cimex lectularius is a blood-feeding re-emerging annoyance pest insect that has the ability to transmit Trypanosoma cruzi under experimental laboratory conditions. Aquaporins (AQPs) are water channel proteins that are essential in biological organisms. C. lectularius are constan...

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Autores principales: Hitoshi Tsujimoto, Joyce M. Sakamoto, Jason L. Rasgon
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/4ec7d6d03f9b42818a591ff75f9b1acb
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spelling oai:doaj.org-article:4ec7d6d03f9b42818a591ff75f9b1acb2021-12-02T16:06:33ZFunctional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius10.1038/s41598-017-03157-22045-2322https://doaj.org/article/4ec7d6d03f9b42818a591ff75f9b1acb2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03157-2https://doaj.org/toc/2045-2322Abstract The bed bug Cimex lectularius is a blood-feeding re-emerging annoyance pest insect that has the ability to transmit Trypanosoma cruzi under experimental laboratory conditions. Aquaporins (AQPs) are water channel proteins that are essential in biological organisms. C. lectularius are constantly exposed to water-related stress, suggesting that AQPs may offer novel control avenues. We identified and cloned four AQPs from C. lectularius, assessed tissue and lifestage-specific expression, and characterized biochemical functions in vitro and in vivo. We identified an efficient water-specific AQP (ClAQP1), two aquaglyceroporins (ClGlp1 and ClGlp2) and a homolog of Drosophila melanogaster big brain (ClBib). ClGlp1 was only functional when co-expressed with the water-specific AQP. Simultaneous RNAi gene silencing of ClAQP1 and ClGlp1 significantly reduced water and urea excretion post blood feeding. The Bib homologue was enriched in embryos, exclusively expressed in ovaries, and when silenced, dramatically increased bug fecundity. Our data demonstrate that AQPs have critical roles in excretion, water homeostasis and reproduction in C. lectularius, and could be potential targets for control in this notorious pest.Hitoshi TsujimotoJoyce M. SakamotoJason L. RasgonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Hitoshi Tsujimoto
Joyce M. Sakamoto
Jason L. Rasgon
Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius
description Abstract The bed bug Cimex lectularius is a blood-feeding re-emerging annoyance pest insect that has the ability to transmit Trypanosoma cruzi under experimental laboratory conditions. Aquaporins (AQPs) are water channel proteins that are essential in biological organisms. C. lectularius are constantly exposed to water-related stress, suggesting that AQPs may offer novel control avenues. We identified and cloned four AQPs from C. lectularius, assessed tissue and lifestage-specific expression, and characterized biochemical functions in vitro and in vivo. We identified an efficient water-specific AQP (ClAQP1), two aquaglyceroporins (ClGlp1 and ClGlp2) and a homolog of Drosophila melanogaster big brain (ClBib). ClGlp1 was only functional when co-expressed with the water-specific AQP. Simultaneous RNAi gene silencing of ClAQP1 and ClGlp1 significantly reduced water and urea excretion post blood feeding. The Bib homologue was enriched in embryos, exclusively expressed in ovaries, and when silenced, dramatically increased bug fecundity. Our data demonstrate that AQPs have critical roles in excretion, water homeostasis and reproduction in C. lectularius, and could be potential targets for control in this notorious pest.
format article
author Hitoshi Tsujimoto
Joyce M. Sakamoto
Jason L. Rasgon
author_facet Hitoshi Tsujimoto
Joyce M. Sakamoto
Jason L. Rasgon
author_sort Hitoshi Tsujimoto
title Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius
title_short Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius
title_full Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius
title_fullStr Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius
title_full_unstemmed Functional characterization of Aquaporin-like genes in the human bed bug Cimex lectularius
title_sort functional characterization of aquaporin-like genes in the human bed bug cimex lectularius
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/4ec7d6d03f9b42818a591ff75f9b1acb
work_keys_str_mv AT hitoshitsujimoto functionalcharacterizationofaquaporinlikegenesinthehumanbedbugcimexlectularius
AT joycemsakamoto functionalcharacterizationofaquaporinlikegenesinthehumanbedbugcimexlectularius
AT jasonlrasgon functionalcharacterizationofaquaporinlikegenesinthehumanbedbugcimexlectularius
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