Nanometer-thin TiO2 enhances skeletal muscle cell phenotype and behavior

Ken Ishizaki*, Yoshihiko Sugita*, Fuminori Iwasa, Hajime Minamikawa, Takeshi Ueno, Masahiro Yamada, Takeo Suzuki, Takahiro OgawaLaboratory for Bone and Implant Sciences, The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospit...

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Autores principales:	Ishizaki K, Sugita Y, Iwasa F, Minamikawa H, Ueno T, Yamada M, Suzuki T, Ogawa T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4ec8fbec05b648e59c9c8867c1f54429
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spelling	oai:doaj.org-article:4ec8fbec05b648e59c9c8867c1f544292021-12-02T00:40:16ZNanometer-thin TiO2 enhances skeletal muscle cell phenotype and behavior1176-91141178-2013https://doaj.org/article/4ec8fbec05b648e59c9c8867c1f544292011-10-01T00:00:00Zhttp://www.dovepress.com/nanometer-thin-tio2-enhances-skeletal-muscle-cell-phenotype-and-behavi-a8398https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Ken Ishizaki, Yoshihiko Sugita, Fuminori Iwasa, Hajime Minamikawa, Takeshi Ueno, Masahiro Yamada, Takeo Suzuki, Takahiro OgawaLaboratory for Bone and Implant Sciences, The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry, Los Angeles, CA, USA*Authors contributed equally to this workBackground: The independent role of the surface chemistry of titanium in determining its biological properties is yet to be determined. Although titanium implants are often in contact with muscle tissue, the interaction of muscle cells with titanium is largely unknown. This study tested the hypotheses that the surface chemistry of clinically established microroughened titanium surfaces could be controllably varied by coating with a minimally thin layer of TiO2 (ideally pico-to-nanometer in thickness) without altering the existing topographical and roughness features, and that the change in superficial chemistry of titanium is effective in improving the biological properties of titanium.Methods and results: Acid-etched microroughened titanium surfaces were coated with TiO2 using slow-rate sputter deposition of molten TiO2 nanoparticles. A TiO2 coating of 300 pm to 6.3 nm increased the surface oxygen on the titanium substrates in a controllable manner, but did not alter the existing microscale architecture and roughness of the substrates. Cells derived from rat skeletal muscles showed increased attachment, spread, adhesion strength, proliferation, gene expression, and collagen production at the initial and early stage of culture on 6.3 nm thick TiO2-coated microroughened titanium surfaces compared with uncoated titanium surfaces.Conclusion: Using an exemplary slow-rate sputter deposition technique of molten TiO2 nanoparticles, this study demonstrated that titanium substrates, even with microscale roughness, can be sufficiently chemically modified to enhance their biological properties without altering the existing microscale morphology. The controllable and exclusive chemical modification technique presented in this study may open a new avenue for surface modifications of titanium-based biomaterials for better cell and tissue affinity and reaction.Keywords: nanotechnology, orthopedic implants, molten TiO2 nanoparticles, surface chemistryIshizaki KSugita YIwasa FMinamikawa HUeno TYamada MSuzuki TOgawa TDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2191-2203 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ishizaki K Sugita Y Iwasa F Minamikawa H Ueno T Yamada M Suzuki T Ogawa T Nanometer-thin TiO2 enhances skeletal muscle cell phenotype and behavior
description	Ken Ishizaki, Yoshihiko Sugita, Fuminori Iwasa, Hajime Minamikawa, Takeshi Ueno, Masahiro Yamada, Takeo Suzuki, Takahiro OgawaLaboratory for Bone and Implant Sciences, The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry, Los Angeles, CA, USA*Authors contributed equally to this workBackground: The independent role of the surface chemistry of titanium in determining its biological properties is yet to be determined. Although titanium implants are often in contact with muscle tissue, the interaction of muscle cells with titanium is largely unknown. This study tested the hypotheses that the surface chemistry of clinically established microroughened titanium surfaces could be controllably varied by coating with a minimally thin layer of TiO2 (ideally pico-to-nanometer in thickness) without altering the existing topographical and roughness features, and that the change in superficial chemistry of titanium is effective in improving the biological properties of titanium.Methods and results: Acid-etched microroughened titanium surfaces were coated with TiO2 using slow-rate sputter deposition of molten TiO2 nanoparticles. A TiO2 coating of 300 pm to 6.3 nm increased the surface oxygen on the titanium substrates in a controllable manner, but did not alter the existing microscale architecture and roughness of the substrates. Cells derived from rat skeletal muscles showed increased attachment, spread, adhesion strength, proliferation, gene expression, and collagen production at the initial and early stage of culture on 6.3 nm thick TiO2-coated microroughened titanium surfaces compared with uncoated titanium surfaces.Conclusion: Using an exemplary slow-rate sputter deposition technique of molten TiO2 nanoparticles, this study demonstrated that titanium substrates, even with microscale roughness, can be sufficiently chemically modified to enhance their biological properties without altering the existing microscale morphology. The controllable and exclusive chemical modification technique presented in this study may open a new avenue for surface modifications of titanium-based biomaterials for better cell and tissue affinity and reaction.Keywords: nanotechnology, orthopedic implants, molten TiO2 nanoparticles, surface chemistry
format	article
author	Ishizaki K Sugita Y Iwasa F Minamikawa H Ueno T Yamada M Suzuki T Ogawa T
author_facet	Ishizaki K Sugita Y Iwasa F Minamikawa H Ueno T Yamada M Suzuki T Ogawa T
author_sort	Ishizaki K
title	Nanometer-thin TiO2 enhances skeletal muscle cell phenotype and behavior
title_short	Nanometer-thin TiO2 enhances skeletal muscle cell phenotype and behavior
title_full	Nanometer-thin TiO2 enhances skeletal muscle cell phenotype and behavior
title_fullStr	Nanometer-thin TiO2 enhances skeletal muscle cell phenotype and behavior
title_full_unstemmed	Nanometer-thin TiO2 enhances skeletal muscle cell phenotype and behavior
title_sort	nanometer-thin tio2 enhances skeletal muscle cell phenotype and behavior
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/4ec8fbec05b648e59c9c8867c1f54429
work_keys_str_mv	AT ishizakik nanometerthintio2enhancesskeletalmusclecellphenotypeandbehavior AT sugitay nanometerthintio2enhancesskeletalmusclecellphenotypeandbehavior AT iwasaf nanometerthintio2enhancesskeletalmusclecellphenotypeandbehavior AT minamikawah nanometerthintio2enhancesskeletalmusclecellphenotypeandbehavior AT uenot nanometerthintio2enhancesskeletalmusclecellphenotypeandbehavior AT yamadam nanometerthintio2enhancesskeletalmusclecellphenotypeandbehavior AT suzukit nanometerthintio2enhancesskeletalmusclecellphenotypeandbehavior AT ogawat nanometerthintio2enhancesskeletalmusclecellphenotypeandbehavior
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Nanometer-thin TiO2 enhances skeletal muscle cell phenotype and behavior

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