Identification and characterization of 63 MicroRNAs in the Asian seabass Lates calcarifer.

<h4>Background</h4>MicroRNAs (miRNAs) play an important role in the regulation of many fundamental biological processes. So far miRNAs have been only identified in a few fish species, although there are over 30,000 fish species living under different environmental conditions on the earth...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jun Hong Xia, Xiao Ping He, Zhi Yi Bai, Gen Hua Yue
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4ec948b9482c4763b34eea8b68bad163
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4ec948b9482c4763b34eea8b68bad163
record_format	dspace
spelling	oai:doaj.org-article:4ec948b9482c4763b34eea8b68bad1632021-11-18T06:57:25ZIdentification and characterization of 63 MicroRNAs in the Asian seabass Lates calcarifer.1932-620310.1371/journal.pone.0017537https://doaj.org/article/4ec948b9482c4763b34eea8b68bad1632011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21412421/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>MicroRNAs (miRNAs) play an important role in the regulation of many fundamental biological processes. So far miRNAs have been only identified in a few fish species, although there are over 30,000 fish species living under different environmental conditions on the earth. Here, we described an approach to identify conserved miRNAs and characterized their expression patterns in different tissues for the first time in a food fish species Asian seabass (Lates calcarifer).<h4>Methodology/principal findings</h4>By combining a bioinformatics analysis with an approach of homolog-based PCR amplification and sequencing, 63 novel miRNAs belonging to 29 conserved miRNA families were identified. Of which, 59 miRNAs were conserved across 10-86 species (E value ≤ 10⁻⁴) and 4 miRNAs were conserved only in fish species. qRT-PCR analysis showed that miR-29, miR-103, miR-125 and several let-7 family members were strongly and ubiquitously expressed in all tissues tested. Interestingly, miR-1, miR-21, miR-183, miR-184 and miR-192 showed highly conserved tissue-specific expression patterns. Exposure of the Asian seabass to lipopolysaccharide (LPS) resulted in up-regulation of over 50% of the identified miRNAs in spleen suggesting the importance of the miRNAs in acute inflammatory immune responses.<h4>Conclusions/significance</h4>The approach used in this study is highly effective for identification of conserved miRNAs. The identification of 63 miRNAs and determination of the spatial expression patterns of these miRNAs are valuable resources for further studies on post-transcriptional gene regulation in Asian seabass and other fish species. Further identification of the target genes of these miRNAs would shed new light on their regulatory roles of microRNAs in fish.Jun Hong XiaXiao Ping HeZhi Yi BaiGen Hua YuePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e17537 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jun Hong Xia Xiao Ping He Zhi Yi Bai Gen Hua Yue Identification and characterization of 63 MicroRNAs in the Asian seabass Lates calcarifer.
description	<h4>Background</h4>MicroRNAs (miRNAs) play an important role in the regulation of many fundamental biological processes. So far miRNAs have been only identified in a few fish species, although there are over 30,000 fish species living under different environmental conditions on the earth. Here, we described an approach to identify conserved miRNAs and characterized their expression patterns in different tissues for the first time in a food fish species Asian seabass (Lates calcarifer).<h4>Methodology/principal findings</h4>By combining a bioinformatics analysis with an approach of homolog-based PCR amplification and sequencing, 63 novel miRNAs belonging to 29 conserved miRNA families were identified. Of which, 59 miRNAs were conserved across 10-86 species (E value ≤ 10⁻⁴) and 4 miRNAs were conserved only in fish species. qRT-PCR analysis showed that miR-29, miR-103, miR-125 and several let-7 family members were strongly and ubiquitously expressed in all tissues tested. Interestingly, miR-1, miR-21, miR-183, miR-184 and miR-192 showed highly conserved tissue-specific expression patterns. Exposure of the Asian seabass to lipopolysaccharide (LPS) resulted in up-regulation of over 50% of the identified miRNAs in spleen suggesting the importance of the miRNAs in acute inflammatory immune responses.<h4>Conclusions/significance</h4>The approach used in this study is highly effective for identification of conserved miRNAs. The identification of 63 miRNAs and determination of the spatial expression patterns of these miRNAs are valuable resources for further studies on post-transcriptional gene regulation in Asian seabass and other fish species. Further identification of the target genes of these miRNAs would shed new light on their regulatory roles of microRNAs in fish.
format	article
author	Jun Hong Xia Xiao Ping He Zhi Yi Bai Gen Hua Yue
author_facet	Jun Hong Xia Xiao Ping He Zhi Yi Bai Gen Hua Yue
author_sort	Jun Hong Xia
title	Identification and characterization of 63 MicroRNAs in the Asian seabass Lates calcarifer.
title_short	Identification and characterization of 63 MicroRNAs in the Asian seabass Lates calcarifer.
title_full	Identification and characterization of 63 MicroRNAs in the Asian seabass Lates calcarifer.
title_fullStr	Identification and characterization of 63 MicroRNAs in the Asian seabass Lates calcarifer.
title_full_unstemmed	Identification and characterization of 63 MicroRNAs in the Asian seabass Lates calcarifer.
title_sort	identification and characterization of 63 micrornas in the asian seabass lates calcarifer.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/4ec948b9482c4763b34eea8b68bad163
work_keys_str_mv	AT junhongxia identificationandcharacterizationof63micrornasintheasianseabasslatescalcarifer AT xiaopinghe identificationandcharacterizationof63micrornasintheasianseabasslatescalcarifer AT zhiyibai identificationandcharacterizationof63micrornasintheasianseabasslatescalcarifer AT genhuayue identificationandcharacterizationof63micrornasintheasianseabasslatescalcarifer
_version_	1718424165522866176

Identification and characterization of 63 MicroRNAs in the Asian seabass Lates calcarifer.

Ejemplares similares