Isolation and identification of cellulolytic bacteria at fibric, hemic and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia

Abstract. Khotimah S, Suharjono, Ardyati T, Nurani Y. 2020. Isolation and identification of cellulolytic bacteria at fibric, hemic, and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia. Biodiversitas 21: 2103-2112. Cellulose degrading bacteria was one of the microbial removers of...

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Autores principales:	Siti Khotimah, Suharjono Suharjono, Tri Ardyati, Yulia Nuraini
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	activity cellulose bacteria, borneo, hemic, peat fibric, sapric Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4ecaad69e1a44d919afe139e096b777b
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spelling	oai:doaj.org-article:4ecaad69e1a44d919afe139e096b777b2021-11-22T00:35:33ZIsolation and identification of cellulolytic bacteria at fibric, hemic and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia1412-033X2085-472210.13057/biodiv/d210538https://doaj.org/article/4ecaad69e1a44d919afe139e096b777b2020-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4933https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Khotimah S, Suharjono, Ardyati T, Nurani Y. 2020. Isolation and identification of cellulolytic bacteria at fibric, hemic, and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia. Biodiversitas 21: 2103-2112. Cellulose degrading bacteria was one of the microbial removers of organic matter contained in the soil into simpler monomers so that it can be utilized by other organisms. The objective of the research was to obtain cellulose-degrading bacteria found on fibric, hemic, and sapric peat in forest and shrubs (oil palm). The bacteria were isolated by pour plate method on 1% CMC media. Selected isolates were assayed quantitatively based on the activity of cellulase enzyme, identified with 16S rDNA. The density of cellulolytic bacteria in the secondary forest peat of fibric, hemic, sapric were 2.1x103 cfu/g, 5.9x104 cfu/g, and 4.9x104 cfu/g whereas, in the area of shrubs/oil palm peat fibric, hemic and sapric 6.9x104 cfu/g, 8.4x104 cfu/g and 3.4x105 cfu/g respectively. There were 19 bacterial isolates that have clear zones around the colony as degradation of cellulose had highest ability to degrade cellulose with clear zones of 5-7 mm. The strain of SB1.1.1 showed highest activity of cellulase enzyme 11.17 U/mL, followed by HH3.1.1 strain and SB2.3 7.83 U/mL. Based on the phylogeny tree, strain SB1.1.1 and HH3.1.1 have the closest kinship relationship with Bacillus cereus with a kinship relationship of 100%, while SB2.3 has the closest kinship relationship with Bacillus stratosphericus with a relationship of 99.85 %.Siti KhotimahSuharjono SuharjonoTri ArdyatiYulia NurainiMBI & UNS Soloarticleactivity cellulose bacteria, borneo, hemic, peat fibric, sapricBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 5 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	activity cellulose bacteria, borneo, hemic, peat fibric, sapric Biology (General) QH301-705.5
spellingShingle	activity cellulose bacteria, borneo, hemic, peat fibric, sapric Biology (General) QH301-705.5 Siti Khotimah Suharjono Suharjono Tri Ardyati Yulia Nuraini Isolation and identification of cellulolytic bacteria at fibric, hemic and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia
description	Abstract. Khotimah S, Suharjono, Ardyati T, Nurani Y. 2020. Isolation and identification of cellulolytic bacteria at fibric, hemic, and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia. Biodiversitas 21: 2103-2112. Cellulose degrading bacteria was one of the microbial removers of organic matter contained in the soil into simpler monomers so that it can be utilized by other organisms. The objective of the research was to obtain cellulose-degrading bacteria found on fibric, hemic, and sapric peat in forest and shrubs (oil palm). The bacteria were isolated by pour plate method on 1% CMC media. Selected isolates were assayed quantitatively based on the activity of cellulase enzyme, identified with 16S rDNA. The density of cellulolytic bacteria in the secondary forest peat of fibric, hemic, sapric were 2.1x103 cfu/g, 5.9x104 cfu/g, and 4.9x104 cfu/g whereas, in the area of shrubs/oil palm peat fibric, hemic and sapric 6.9x104 cfu/g, 8.4x104 cfu/g and 3.4x105 cfu/g respectively. There were 19 bacterial isolates that have clear zones around the colony as degradation of cellulose had highest ability to degrade cellulose with clear zones of 5-7 mm. The strain of SB1.1.1 showed highest activity of cellulase enzyme 11.17 U/mL, followed by HH3.1.1 strain and SB2.3 7.83 U/mL. Based on the phylogeny tree, strain SB1.1.1 and HH3.1.1 have the closest kinship relationship with Bacillus cereus with a kinship relationship of 100%, while SB2.3 has the closest kinship relationship with Bacillus stratosphericus with a relationship of 99.85 %.
format	article
author	Siti Khotimah Suharjono Suharjono Tri Ardyati Yulia Nuraini
author_facet	Siti Khotimah Suharjono Suharjono Tri Ardyati Yulia Nuraini
author_sort	Siti Khotimah
title	Isolation and identification of cellulolytic bacteria at fibric, hemic and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia
title_short	Isolation and identification of cellulolytic bacteria at fibric, hemic and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia
title_full	Isolation and identification of cellulolytic bacteria at fibric, hemic and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia
title_fullStr	Isolation and identification of cellulolytic bacteria at fibric, hemic and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia
title_full_unstemmed	Isolation and identification of cellulolytic bacteria at fibric, hemic and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia
title_sort	isolation and identification of cellulolytic bacteria at fibric, hemic and sapric peat in teluk bakung peatland, kubu raya district, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/4ecaad69e1a44d919afe139e096b777b
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Isolation and identification of cellulolytic bacteria at fibric, hemic and sapric peat in Teluk Bakung Peatland, Kubu Raya District, Indonesia

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