Biomolecular characteristics of Yersinia pseudotuberculosis and Yersinia enterocolitica isolated in Siberia and in the Far East

A special feature of Y. pseudotuberculosis strains is its biochemical uniformity irrespective of the time and location of the causative agent isolation and the existence of 21 serological variants. Y. enterocolitica is a quite a heterogeneous species and is classified into 6 biochemical types associ...

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Autores principales:	T. V. Karimova, V. T. Klimov, M. V. Chesnokova
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2016
Materias:	yersinia pseudotuberculosis yersinia enterocolitica pathogenicity factor strains genotyping Science Q
Acceso en línea:	https://doaj.org/article/4ed074326cbd4df2a1b529356b93abcf
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spelling	oai:doaj.org-article:4ed074326cbd4df2a1b529356b93abcf2021-11-23T06:14:35ZBiomolecular characteristics of Yersinia pseudotuberculosis and Yersinia enterocolitica isolated in Siberia and in the Far East2541-94202587-959610.12737/21612https://doaj.org/article/4ed074326cbd4df2a1b529356b93abcf2016-05-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/228https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596A special feature of Y. pseudotuberculosis strains is its biochemical uniformity irrespective of the time and location of the causative agent isolation and the existence of 21 serological variants. Y. enterocolitica is a quite a heterogeneous species and is classified into 6 biochemical types associated with 29 serological variants. 221 Y. pseudotuberculosis and 447 Y. enterocolitica strains in total isolated in Siberia and in the Far East were characterized. Y. pseudotuberculosis genotype dominating in the Siberian and Far Eastern regions is presented by 0:1b serotype of the first genogroup (pYV+, ympA+, HPI-) in two- (47:82 МDa) or single-plasmid (47 МDа) variants. Ribotyping and fingerprinting revealed 8 and 10 Y. pseudotuberculosis genotypes, respectively, that indicated relative heterogeneity of the circulated strains. Regional difference of ribotypes and fingerprints was noted. 401 of447 Y. enterocolitica strains were classified as biotype A1 including 11 serotypes (0:4,32; 0:4,44; 0:5; 0:6,30; 0:6,31; 0;7,8; 0:12,25; 0:13,7; O:19,8; 0:41,43) and 46 strains belonged to biotypes 2-4 of O:3 and O:9 serotypes. Y. enterocolitica strains of biotypes 1A were isolated both from the environments, animals and patient samples as like the representatives of biotypes 2-4. The differentiating tests of fucose and sorbose made it possible to identify two species new for the Russian Federation - Y. mollaretii and Y. bercovierii. Y. enterocolitica biotypes 2-4 carried pYV plasmid and chromosomal ail, ystA virulence genes. These strains were referred to phagotypes X3 (2/0:9) and VIII (4/0:3) and also to phagotype Xz (3/0:3), unique for Russia. Y. enterocolitica biotype 1A containing ystB thermostable toxin gene was confirmed to be an infectious etiological agent.T. V. KarimovaV. T. KlimovM. V. ChesnokovaScientific Сentre for Family Health and Human Reproduction Problemsarticleyersinia pseudotuberculosisyersinia enterocoliticayersinia pseudotuberculosisyersinia enterocoliticapathogenicity factorstrains genotypingScienceQRUActa Biomedica Scientifica, Vol 1, Iss 3(1), Pp 60-64 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	yersinia pseudotuberculosis yersinia enterocolitica yersinia pseudotuberculosis yersinia enterocolitica pathogenicity factor strains genotyping Science Q
spellingShingle	yersinia pseudotuberculosis yersinia enterocolitica yersinia pseudotuberculosis yersinia enterocolitica pathogenicity factor strains genotyping Science Q T. V. Karimova V. T. Klimov M. V. Chesnokova Biomolecular characteristics of Yersinia pseudotuberculosis and Yersinia enterocolitica isolated in Siberia and in the Far East
description	A special feature of Y. pseudotuberculosis strains is its biochemical uniformity irrespective of the time and location of the causative agent isolation and the existence of 21 serological variants. Y. enterocolitica is a quite a heterogeneous species and is classified into 6 biochemical types associated with 29 serological variants. 221 Y. pseudotuberculosis and 447 Y. enterocolitica strains in total isolated in Siberia and in the Far East were characterized. Y. pseudotuberculosis genotype dominating in the Siberian and Far Eastern regions is presented by 0:1b serotype of the first genogroup (pYV+, ympA+, HPI-) in two- (47:82 МDa) or single-plasmid (47 МDа) variants. Ribotyping and fingerprinting revealed 8 and 10 Y. pseudotuberculosis genotypes, respectively, that indicated relative heterogeneity of the circulated strains. Regional difference of ribotypes and fingerprints was noted. 401 of447 Y. enterocolitica strains were classified as biotype A1 including 11 serotypes (0:4,32; 0:4,44; 0:5; 0:6,30; 0:6,31; 0;7,8; 0:12,25; 0:13,7; O:19,8; 0:41,43) and 46 strains belonged to biotypes 2-4 of O:3 and O:9 serotypes. Y. enterocolitica strains of biotypes 1A were isolated both from the environments, animals and patient samples as like the representatives of biotypes 2-4. The differentiating tests of fucose and sorbose made it possible to identify two species new for the Russian Federation - Y. mollaretii and Y. bercovierii. Y. enterocolitica biotypes 2-4 carried pYV plasmid and chromosomal ail, ystA virulence genes. These strains were referred to phagotypes X3 (2/0:9) and VIII (4/0:3) and also to phagotype Xz (3/0:3), unique for Russia. Y. enterocolitica biotype 1A containing ystB thermostable toxin gene was confirmed to be an infectious etiological agent.
format	article
author	T. V. Karimova V. T. Klimov M. V. Chesnokova
author_facet	T. V. Karimova V. T. Klimov M. V. Chesnokova
author_sort	T. V. Karimova
title	Biomolecular characteristics of Yersinia pseudotuberculosis and Yersinia enterocolitica isolated in Siberia and in the Far East
title_short	Biomolecular characteristics of Yersinia pseudotuberculosis and Yersinia enterocolitica isolated in Siberia and in the Far East
title_full	Biomolecular characteristics of Yersinia pseudotuberculosis and Yersinia enterocolitica isolated in Siberia and in the Far East
title_fullStr	Biomolecular characteristics of Yersinia pseudotuberculosis and Yersinia enterocolitica isolated in Siberia and in the Far East
title_full_unstemmed	Biomolecular characteristics of Yersinia pseudotuberculosis and Yersinia enterocolitica isolated in Siberia and in the Far East
title_sort	biomolecular characteristics of yersinia pseudotuberculosis and yersinia enterocolitica isolated in siberia and in the far east
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2016
url	https://doaj.org/article/4ed074326cbd4df2a1b529356b93abcf
work_keys_str_mv	AT tvkarimova biomolecularcharacteristicsofyersiniapseudotuberculosisandyersiniaenterocoliticaisolatedinsiberiaandinthefareast AT vtklimov biomolecularcharacteristicsofyersiniapseudotuberculosisandyersiniaenterocoliticaisolatedinsiberiaandinthefareast AT mvchesnokova biomolecularcharacteristicsofyersiniapseudotuberculosisandyersiniaenterocoliticaisolatedinsiberiaandinthefareast
_version_	1718417004007784448

Biomolecular characteristics of Yersinia pseudotuberculosis and Yersinia enterocolitica isolated in Siberia and in the Far East

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