Serum and Urine Copper – Contamination and Stability

Pre-analytical factors of variation need to be carefully considered and investigated in efforts to harmonize all aspects of the total testing process. This study aimed to evaluate contamination and stability in copper (Cu) analysis of serum and urine by flame atomic absorption spectroscopy (FAAS) an...

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Autores principales:	Dimitrova Ivanova I., Atanasova B., Dragneva S., Vladimirova L., Krastev Z., Kostadinova A., Ivanova A., Tzatchev K.
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2015
Materias:	cu atomic absorption pre-analytical phase contamination stability dpenicillamine Medicine R
Acceso en línea:	https://doaj.org/article/4ed4ac6a1cbf4529a202562b9db7923b
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spelling	oai:doaj.org-article:4ed4ac6a1cbf4529a202562b9db7923b2021-12-02T17:31:48ZSerum and Urine Copper – Contamination and Stability0324-175010.1515/amb-2015-0018https://doaj.org/article/4ed4ac6a1cbf4529a202562b9db7923b2015-11-01T00:00:00Zhttps://doi.org/10.1515/amb-2015-0018https://doaj.org/toc/0324-1750Pre-analytical factors of variation need to be carefully considered and investigated in efforts to harmonize all aspects of the total testing process. This study aimed to evaluate contamination and stability in copper (Cu) analysis of serum and urine by flame atomic absorption spectroscopy (FAAS) and to compare the stability of urine Cu in controls and in D-penicillamine (D-PA) administration. Cu was measured by AAnalyst 400, Perkin Elmer, USA. Blood was collected in BD Vacutainer®SSTTM II Advance tubes and BD Vacutainer® Trace Element tubes. Sterile polyethylene and polypropylene vessels for collection, transportation, storage and preliminary preparation of samples were used in urinalysis. Stability in serum and 24 h urine was evaluated in two temperature regimens: 15-25°C and 2-8°C, for particular time of storage. No significant differences (p = 0.20) in Cu concentration was found between the two types of tested tubes with patient`s sera. The stability of the samples (serum and urine) was better at refrigeration temperature. In urine the stability was better in D-PA administration.Standardization of Cu analysis could be achieved by assessing the aspects of pre-analytical factors of variations.Dimitrova Ivanova I.Atanasova B.Dragneva S.Vladimirova L.Krastev Z.Kostadinova A.Ivanova A.Tzatchev K.Sciendoarticlecuatomic absorptionpre-analytical phasecontaminationstabilitydpenicillamineMedicineRENActa Medica Bulgarica, Vol 42, Iss 2, Pp 49-60 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	cu atomic absorption pre-analytical phase contamination stability dpenicillamine Medicine R
spellingShingle	cu atomic absorption pre-analytical phase contamination stability dpenicillamine Medicine R Dimitrova Ivanova I. Atanasova B. Dragneva S. Vladimirova L. Krastev Z. Kostadinova A. Ivanova A. Tzatchev K. Serum and Urine Copper – Contamination and Stability
description	Pre-analytical factors of variation need to be carefully considered and investigated in efforts to harmonize all aspects of the total testing process. This study aimed to evaluate contamination and stability in copper (Cu) analysis of serum and urine by flame atomic absorption spectroscopy (FAAS) and to compare the stability of urine Cu in controls and in D-penicillamine (D-PA) administration. Cu was measured by AAnalyst 400, Perkin Elmer, USA. Blood was collected in BD Vacutainer®SSTTM II Advance tubes and BD Vacutainer® Trace Element tubes. Sterile polyethylene and polypropylene vessels for collection, transportation, storage and preliminary preparation of samples were used in urinalysis. Stability in serum and 24 h urine was evaluated in two temperature regimens: 15-25°C and 2-8°C, for particular time of storage. No significant differences (p = 0.20) in Cu concentration was found between the two types of tested tubes with patient`s sera. The stability of the samples (serum and urine) was better at refrigeration temperature. In urine the stability was better in D-PA administration.Standardization of Cu analysis could be achieved by assessing the aspects of pre-analytical factors of variations.
format	article
author	Dimitrova Ivanova I. Atanasova B. Dragneva S. Vladimirova L. Krastev Z. Kostadinova A. Ivanova A. Tzatchev K.
author_facet	Dimitrova Ivanova I. Atanasova B. Dragneva S. Vladimirova L. Krastev Z. Kostadinova A. Ivanova A. Tzatchev K.
author_sort	Dimitrova Ivanova I.
title	Serum and Urine Copper – Contamination and Stability
title_short	Serum and Urine Copper – Contamination and Stability
title_full	Serum and Urine Copper – Contamination and Stability
title_fullStr	Serum and Urine Copper – Contamination and Stability
title_full_unstemmed	Serum and Urine Copper – Contamination and Stability
title_sort	serum and urine copper – contamination and stability
publisher	Sciendo
publishDate	2015
url	https://doaj.org/article/4ed4ac6a1cbf4529a202562b9db7923b
work_keys_str_mv	AT dimitrovaivanovai serumandurinecoppercontaminationandstability AT atanasovab serumandurinecoppercontaminationandstability AT dragnevas serumandurinecoppercontaminationandstability AT vladimiroval serumandurinecoppercontaminationandstability AT krastevz serumandurinecoppercontaminationandstability AT kostadinovaa serumandurinecoppercontaminationandstability AT ivanovaa serumandurinecoppercontaminationandstability AT tzatchevk serumandurinecoppercontaminationandstability
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Serum and Urine Copper – Contamination and Stability

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