A novel highly quantitative and reproducible assay for the detection of anti-SARS-CoV-2 IgG and IgM antibodies
Abstract The quantitative range and reproducibility of current serological tests for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) are not optimized. Herein, we developed a diagnostic test that detects SARS-CoV-2 IgG and IgM with high quantitativeness and reproducibility and low inter...
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Nature Portfolio
2021
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oai:doaj.org-article:4ee0077c063d48edb7287aa6722692e42021-12-02T11:37:19ZA novel highly quantitative and reproducible assay for the detection of anti-SARS-CoV-2 IgG and IgM antibodies10.1038/s41598-021-84387-32045-2322https://doaj.org/article/4ee0077c063d48edb7287aa6722692e42021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84387-3https://doaj.org/toc/2045-2322Abstract The quantitative range and reproducibility of current serological tests for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) are not optimized. Herein, we developed a diagnostic test that detects SARS-CoV-2 IgG and IgM with high quantitativeness and reproducibility and low interference. The system was based on the high-sensitivity chemiluminescence enzyme immunoassay (HISCL) platform and detects IgG and IgM specific to SARS-CoV-2 spike and nucleocapsid proteins. Quantification accuracy and reproducibility were evaluated using serially diluted samples from 60 SARS-CoV-2-infected patients. Assay performance was evaluated using serum samples from the SARS-CoV-2-infected patients and 500 SARS-CoV-2-negative serum samples collected before the emergence of SARS-CoV-2. The system showed high quantification accuracy (range, 102), high reproducibility (within 5%), and no cross-reaction between SARS1- and MERS-S proteins. Detection accuracy was 98.3% and 93.3% for IgG and IgM against spike proteins and 100% and 71.7% for IgG and IgM against nucleocapsid proteins, respectively. Mean antibody levels were > 10 times that in negative samples upon admission and > 100 times that at convalescent periods. Clinical severity upon admission was not correlated with IgG or IgM levels. This highly quantitative, reproducible assay system with high clinical performance may help analyze temporal serological/immunological profiles of SARS-CoV-2 infection and SARS-CoV-2 vaccine effectiveness.Kenta NodaKouki MatsudaShigehiro YagishitaKenji MaedaYutaro AkiyamaJunko Terada-HirashimaHiromichi MatsushitaSatoshi IwataKazuto YamashitaYusuke AtarashiShunsuke WatanabeNobuyuki IdeTomokazu YoshidaNorio OhmagariHiroaki MitsuyaAkinobu HamadaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) |
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DOAJ |
| collection |
DOAJ |
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EN |
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Medicine R Science Q |
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Medicine R Science Q Kenta Noda Kouki Matsuda Shigehiro Yagishita Kenji Maeda Yutaro Akiyama Junko Terada-Hirashima Hiromichi Matsushita Satoshi Iwata Kazuto Yamashita Yusuke Atarashi Shunsuke Watanabe Nobuyuki Ide Tomokazu Yoshida Norio Ohmagari Hiroaki Mitsuya Akinobu Hamada A novel highly quantitative and reproducible assay for the detection of anti-SARS-CoV-2 IgG and IgM antibodies |
| description |
Abstract The quantitative range and reproducibility of current serological tests for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) are not optimized. Herein, we developed a diagnostic test that detects SARS-CoV-2 IgG and IgM with high quantitativeness and reproducibility and low interference. The system was based on the high-sensitivity chemiluminescence enzyme immunoassay (HISCL) platform and detects IgG and IgM specific to SARS-CoV-2 spike and nucleocapsid proteins. Quantification accuracy and reproducibility were evaluated using serially diluted samples from 60 SARS-CoV-2-infected patients. Assay performance was evaluated using serum samples from the SARS-CoV-2-infected patients and 500 SARS-CoV-2-negative serum samples collected before the emergence of SARS-CoV-2. The system showed high quantification accuracy (range, 102), high reproducibility (within 5%), and no cross-reaction between SARS1- and MERS-S proteins. Detection accuracy was 98.3% and 93.3% for IgG and IgM against spike proteins and 100% and 71.7% for IgG and IgM against nucleocapsid proteins, respectively. Mean antibody levels were > 10 times that in negative samples upon admission and > 100 times that at convalescent periods. Clinical severity upon admission was not correlated with IgG or IgM levels. This highly quantitative, reproducible assay system with high clinical performance may help analyze temporal serological/immunological profiles of SARS-CoV-2 infection and SARS-CoV-2 vaccine effectiveness. |
| format |
article |
| author |
Kenta Noda Kouki Matsuda Shigehiro Yagishita Kenji Maeda Yutaro Akiyama Junko Terada-Hirashima Hiromichi Matsushita Satoshi Iwata Kazuto Yamashita Yusuke Atarashi Shunsuke Watanabe Nobuyuki Ide Tomokazu Yoshida Norio Ohmagari Hiroaki Mitsuya Akinobu Hamada |
| author_facet |
Kenta Noda Kouki Matsuda Shigehiro Yagishita Kenji Maeda Yutaro Akiyama Junko Terada-Hirashima Hiromichi Matsushita Satoshi Iwata Kazuto Yamashita Yusuke Atarashi Shunsuke Watanabe Nobuyuki Ide Tomokazu Yoshida Norio Ohmagari Hiroaki Mitsuya Akinobu Hamada |
| author_sort |
Kenta Noda |
| title |
A novel highly quantitative and reproducible assay for the detection of anti-SARS-CoV-2 IgG and IgM antibodies |
| title_short |
A novel highly quantitative and reproducible assay for the detection of anti-SARS-CoV-2 IgG and IgM antibodies |
| title_full |
A novel highly quantitative and reproducible assay for the detection of anti-SARS-CoV-2 IgG and IgM antibodies |
| title_fullStr |
A novel highly quantitative and reproducible assay for the detection of anti-SARS-CoV-2 IgG and IgM antibodies |
| title_full_unstemmed |
A novel highly quantitative and reproducible assay for the detection of anti-SARS-CoV-2 IgG and IgM antibodies |
| title_sort |
novel highly quantitative and reproducible assay for the detection of anti-sars-cov-2 igg and igm antibodies |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/4ee0077c063d48edb7287aa6722692e4 |
| work_keys_str_mv |
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