CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism

The use of site-specific gene insertion is a powerful method for investigating gene function. Here the authors describe CRISPaint, a universal tagging system using CRISPR-Cas9 to insert genes in an NHEJ dependent manner.

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Detalles Bibliográficos
Autores principales: Jonathan L. Schmid-Burgk, Klara Höning, Thomas S. Ebert, Veit Hornung
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/4ee6796f750540338a9604189f2c3ea7
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Sumario:The use of site-specific gene insertion is a powerful method for investigating gene function. Here the authors describe CRISPaint, a universal tagging system using CRISPR-Cas9 to insert genes in an NHEJ dependent manner.