CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism

The use of site-specific gene insertion is a powerful method for investigating gene function. Here the authors describe CRISPaint, a universal tagging system using CRISPR-Cas9 to insert genes in an NHEJ dependent manner.

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Detalles Bibliográficos
Autores principales:	Jonathan L. Schmid-Burgk, Klara Höning, Thomas S. Ebert, Veit Hornung
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/4ee6796f750540338a9604189f2c3ea7
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spelling	oai:doaj.org-article:4ee6796f750540338a9604189f2c3ea72021-12-02T15:34:54ZCRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism10.1038/ncomms123382041-1723https://doaj.org/article/4ee6796f750540338a9604189f2c3ea72016-07-01T00:00:00Zhttps://doi.org/10.1038/ncomms12338https://doaj.org/toc/2041-1723The use of site-specific gene insertion is a powerful method for investigating gene function. Here the authors describe CRISPaint, a universal tagging system using CRISPR-Cas9 to insert genes in an NHEJ dependent manner.Jonathan L. Schmid-BurgkKlara HöningThomas S. EbertVeit HornungNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-12 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jonathan L. Schmid-Burgk Klara Höning Thomas S. Ebert Veit Hornung CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism
description	The use of site-specific gene insertion is a powerful method for investigating gene function. Here the authors describe CRISPaint, a universal tagging system using CRISPR-Cas9 to insert genes in an NHEJ dependent manner.
format	article
author	Jonathan L. Schmid-Burgk Klara Höning Thomas S. Ebert Veit Hornung
author_facet	Jonathan L. Schmid-Burgk Klara Höning Thomas S. Ebert Veit Hornung
author_sort	Jonathan L. Schmid-Burgk
title	CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism
title_short	CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism
title_full	CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism
title_fullStr	CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism
title_full_unstemmed	CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism
title_sort	crispaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/4ee6796f750540338a9604189f2c3ea7
work_keys_str_mv	AT jonathanlschmidburgk crispaintallowsmodularbasespecificgenetaggingusingaligase4dependentmechanism AT klarahoning crispaintallowsmodularbasespecificgenetaggingusingaligase4dependentmechanism AT thomassebert crispaintallowsmodularbasespecificgenetaggingusingaligase4dependentmechanism AT veithornung crispaintallowsmodularbasespecificgenetaggingusingaligase4dependentmechanism
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CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism

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