CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism
The use of site-specific gene insertion is a powerful method for investigating gene function. Here the authors describe CRISPaint, a universal tagging system using CRISPR-Cas9 to insert genes in an NHEJ dependent manner.
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Autores principales: | Jonathan L. Schmid-Burgk, Klara Höning, Thomas S. Ebert, Veit Hornung |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2016
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Materias: | |
Acceso en línea: | https://doaj.org/article/4ee6796f750540338a9604189f2c3ea7 |
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