Metabolomics reveals biotic and abiotic elicitor effects on the soft coral Sarcophyton ehrenbergi terpenoid content

Abstract The effects of six biotic and abiotic elicitors, i.e. MeJA (methyl jasmonate), SA (salicylic acid), ZnCl2, glutathione and β-glucan BG (fungal elicitor), and wounding, on the secondary metabolite accumulation in the soft coral Sarcophyton ehrenbergi were assessed. Upon elicitation, metaboli...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mohamed A. Farag, Dalia A. Al-Mahdy, Achim Meyer, Hildegard Westphal, Ludger A. Wessjohann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4eee3be68e304a88afe894b35c63b2ec
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4eee3be68e304a88afe894b35c63b2ec
record_format	dspace
spelling	oai:doaj.org-article:4eee3be68e304a88afe894b35c63b2ec2021-12-02T15:05:07ZMetabolomics reveals biotic and abiotic elicitor effects on the soft coral Sarcophyton ehrenbergi terpenoid content10.1038/s41598-017-00527-82045-2322https://doaj.org/article/4eee3be68e304a88afe894b35c63b2ec2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00527-8https://doaj.org/toc/2045-2322Abstract The effects of six biotic and abiotic elicitors, i.e. MeJA (methyl jasmonate), SA (salicylic acid), ZnCl2, glutathione and β-glucan BG (fungal elicitor), and wounding, on the secondary metabolite accumulation in the soft coral Sarcophyton ehrenbergi were assessed. Upon elicitation, metabolites were extracted and analysed by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Except for MeJA, no differences in photosynthetic efficiency were observed after treatments, suggesting the absence of a remarkable stress on primary production. Chemometric analyses of UPLC-MS data showed clear segregation of SA and ZnCl2 elicited samples at 24 and 48 h post elicitation. Levels of acetylated diterpene and sterol viz., sarcophytonolide I and cholesteryl acetate, was increased in ZnCl2 and SA groups, respectively, suggesting an activation of specific acetyl transferases. Post elicitation, sarcophytonolide I level increased 132 and 17-folds at 48 h in 0.1 mM SA and 1 mM ZnCl2 groups, respectively. Interestingly, decrease in sarcophine, a major diterpene was observed only in response to ZnCl2, whereas no change was observed in sesquiterpene content following treatments. To the best of our knowledge, this study provides the first documentation for elicitation effects on a soft corals secondary metabolome and suggests that SA could be applied to increase diterpenoid levels in corals.Mohamed A. FaragDalia A. Al-MahdyAchim MeyerHildegard WestphalLudger A. WessjohannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mohamed A. Farag Dalia A. Al-Mahdy Achim Meyer Hildegard Westphal Ludger A. Wessjohann Metabolomics reveals biotic and abiotic elicitor effects on the soft coral Sarcophyton ehrenbergi terpenoid content
description	Abstract The effects of six biotic and abiotic elicitors, i.e. MeJA (methyl jasmonate), SA (salicylic acid), ZnCl2, glutathione and β-glucan BG (fungal elicitor), and wounding, on the secondary metabolite accumulation in the soft coral Sarcophyton ehrenbergi were assessed. Upon elicitation, metabolites were extracted and analysed by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). Except for MeJA, no differences in photosynthetic efficiency were observed after treatments, suggesting the absence of a remarkable stress on primary production. Chemometric analyses of UPLC-MS data showed clear segregation of SA and ZnCl2 elicited samples at 24 and 48 h post elicitation. Levels of acetylated diterpene and sterol viz., sarcophytonolide I and cholesteryl acetate, was increased in ZnCl2 and SA groups, respectively, suggesting an activation of specific acetyl transferases. Post elicitation, sarcophytonolide I level increased 132 and 17-folds at 48 h in 0.1 mM SA and 1 mM ZnCl2 groups, respectively. Interestingly, decrease in sarcophine, a major diterpene was observed only in response to ZnCl2, whereas no change was observed in sesquiterpene content following treatments. To the best of our knowledge, this study provides the first documentation for elicitation effects on a soft corals secondary metabolome and suggests that SA could be applied to increase diterpenoid levels in corals.
format	article
author	Mohamed A. Farag Dalia A. Al-Mahdy Achim Meyer Hildegard Westphal Ludger A. Wessjohann
author_facet	Mohamed A. Farag Dalia A. Al-Mahdy Achim Meyer Hildegard Westphal Ludger A. Wessjohann
author_sort	Mohamed A. Farag
title	Metabolomics reveals biotic and abiotic elicitor effects on the soft coral Sarcophyton ehrenbergi terpenoid content
title_short	Metabolomics reveals biotic and abiotic elicitor effects on the soft coral Sarcophyton ehrenbergi terpenoid content
title_full	Metabolomics reveals biotic and abiotic elicitor effects on the soft coral Sarcophyton ehrenbergi terpenoid content
title_fullStr	Metabolomics reveals biotic and abiotic elicitor effects on the soft coral Sarcophyton ehrenbergi terpenoid content
title_full_unstemmed	Metabolomics reveals biotic and abiotic elicitor effects on the soft coral Sarcophyton ehrenbergi terpenoid content
title_sort	metabolomics reveals biotic and abiotic elicitor effects on the soft coral sarcophyton ehrenbergi terpenoid content
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4eee3be68e304a88afe894b35c63b2ec
work_keys_str_mv	AT mohamedafarag metabolomicsrevealsbioticandabioticelicitoreffectsonthesoftcoralsarcophytonehrenbergiterpenoidcontent AT daliaaalmahdy metabolomicsrevealsbioticandabioticelicitoreffectsonthesoftcoralsarcophytonehrenbergiterpenoidcontent AT achimmeyer metabolomicsrevealsbioticandabioticelicitoreffectsonthesoftcoralsarcophytonehrenbergiterpenoidcontent AT hildegardwestphal metabolomicsrevealsbioticandabioticelicitoreffectsonthesoftcoralsarcophytonehrenbergiterpenoidcontent AT ludgerawessjohann metabolomicsrevealsbioticandabioticelicitoreffectsonthesoftcoralsarcophytonehrenbergiterpenoidcontent
_version_	1718388953010143232

Metabolomics reveals biotic and abiotic elicitor effects on the soft coral Sarcophyton ehrenbergi terpenoid content

Ejemplares similares