Investigation and optimization of formulation parameters on preparation of targeted anti-CD205 tailored PLGA nanoparticles

Sheikh Tasnim Jahan, Azita Haddadi Division of Pharmacy, College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, SK, Canada Abstract: The purpose of this study was to assess the effect of various formulation parameters on anti-CD205 antibody decorated poly(D, L-lactide c...

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Autores principales:	Jahan ST, Haddadi A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Nanoparticle anti-CD205 PLGA dendritic cells Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/4ef36678163b4bfb831627ebff3fc4db
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spelling	oai:doaj.org-article:4ef36678163b4bfb831627ebff3fc4db2021-12-02T05:27:26ZInvestigation and optimization of formulation parameters on preparation of targeted anti-CD205 tailored PLGA nanoparticles1178-2013https://doaj.org/article/4ef36678163b4bfb831627ebff3fc4db2015-12-01T00:00:00Zhttps://www.dovepress.com/investigation-and-optimization-of-formulation-parameters-on-preparatio-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sheikh Tasnim Jahan, Azita Haddadi Division of Pharmacy, College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, SK, Canada Abstract: The purpose of this study was to assess the effect of various formulation parameters on anti-CD205 antibody decorated poly(D, L-lactide co-glycolide) (PLGA) nanoparticles (NPs) in terms of their ability to target dendritic cells (DCs). In brief, emulsification solvent evaporation technique was adapted to design NP formulations using two different viscosity grades (low and high) of both ester and carboxylic acid terminated PLGA. Incorporation of ligand was achieved following physical adsorption or chemical conjugation processes. The physicochemical characterizations of formulations were executed to assess the effects of different solvents (chloroform and ethyl acetate), stabilizer percentage, polymer types, polymer viscosities, ligand-NP bonding types, cross-linkers, and cryoprotectants (sucrose and trehalose). Modification of any of these parameters shows significant improvement of physicochemical properties of NPs. Ethyl acetate was the solvent of choice for the formulations to ensure better emulsion formation. Infrared spectroscopy confirmed the presence of anti-CD205 antibody in the NP formulation. Finally, cytotoxicity assay confirmed the safety profile of the NPs for DCs. Thus, ligand modified structurally concealed PLGA NPs is a promising delivery tool for targeting DCs in vivo. Keywords: nanoparticle, anti-CD205, PLGA, dendritic cellsJahan STHaddadi ADove Medical PressarticleNanoparticleanti-CD205PLGAdendritic cellsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 7371-7384 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nanoparticle anti-CD205 PLGA dendritic cells Medicine (General) R5-920
spellingShingle	Nanoparticle anti-CD205 PLGA dendritic cells Medicine (General) R5-920 Jahan ST Haddadi A Investigation and optimization of formulation parameters on preparation of targeted anti-CD205 tailored PLGA nanoparticles
description	Sheikh Tasnim Jahan, Azita Haddadi Division of Pharmacy, College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, SK, Canada Abstract: The purpose of this study was to assess the effect of various formulation parameters on anti-CD205 antibody decorated poly(D, L-lactide co-glycolide) (PLGA) nanoparticles (NPs) in terms of their ability to target dendritic cells (DCs). In brief, emulsification solvent evaporation technique was adapted to design NP formulations using two different viscosity grades (low and high) of both ester and carboxylic acid terminated PLGA. Incorporation of ligand was achieved following physical adsorption or chemical conjugation processes. The physicochemical characterizations of formulations were executed to assess the effects of different solvents (chloroform and ethyl acetate), stabilizer percentage, polymer types, polymer viscosities, ligand-NP bonding types, cross-linkers, and cryoprotectants (sucrose and trehalose). Modification of any of these parameters shows significant improvement of physicochemical properties of NPs. Ethyl acetate was the solvent of choice for the formulations to ensure better emulsion formation. Infrared spectroscopy confirmed the presence of anti-CD205 antibody in the NP formulation. Finally, cytotoxicity assay confirmed the safety profile of the NPs for DCs. Thus, ligand modified structurally concealed PLGA NPs is a promising delivery tool for targeting DCs in vivo. Keywords: nanoparticle, anti-CD205, PLGA, dendritic cells
format	article
author	Jahan ST Haddadi A
author_facet	Jahan ST Haddadi A
author_sort	Jahan ST
title	Investigation and optimization of formulation parameters on preparation of targeted anti-CD205 tailored PLGA nanoparticles
title_short	Investigation and optimization of formulation parameters on preparation of targeted anti-CD205 tailored PLGA nanoparticles
title_full	Investigation and optimization of formulation parameters on preparation of targeted anti-CD205 tailored PLGA nanoparticles
title_fullStr	Investigation and optimization of formulation parameters on preparation of targeted anti-CD205 tailored PLGA nanoparticles
title_full_unstemmed	Investigation and optimization of formulation parameters on preparation of targeted anti-CD205 tailored PLGA nanoparticles
title_sort	investigation and optimization of formulation parameters on preparation of targeted anti-cd205 tailored plga nanoparticles
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/4ef36678163b4bfb831627ebff3fc4db
work_keys_str_mv	AT jahanst investigationandoptimizationofformulationparametersonpreparationoftargetedanticd205tailoredplgananoparticles AT haddadia investigationandoptimizationofformulationparametersonpreparationoftargetedanticd205tailoredplgananoparticles
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Investigation and optimization of formulation parameters on preparation of targeted anti-CD205 tailored PLGA nanoparticles

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