High throughput multiple locus variable number of tandem repeat analysis (MLVA) of Staphylococcus aureus from human, animal and food sources.

Staphylococcus aureus is a major human pathogen, a relevant pathogen in veterinary medicine, and a major cause of food poisoning. Epidemiological investigation tools are needed to establish surveillance of S. aureus strains in humans, animals and food. In this study, we investigated 145 S. aureus is...

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Autores principales:	Daniel Sobral, Stefan Schwarz, Dominique Bergonier, Anne Brisabois, Andrea T Feßler, Florence B Gilbert, Kristina Kadlec, Benoit Lebeau, Fabienne Loisy-Hamon, Michaël Treilles, Christine Pourcel, Gilles Vergnaud
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4ef4c825b66340bfa6dd91ed6f63c3c0
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spelling	oai:doaj.org-article:4ef4c825b66340bfa6dd91ed6f63c3c02021-11-18T07:20:00ZHigh throughput multiple locus variable number of tandem repeat analysis (MLVA) of Staphylococcus aureus from human, animal and food sources.1932-620310.1371/journal.pone.0033967https://doaj.org/article/4ef4c825b66340bfa6dd91ed6f63c3c02012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22567085/?tool=EBIhttps://doaj.org/toc/1932-6203Staphylococcus aureus is a major human pathogen, a relevant pathogen in veterinary medicine, and a major cause of food poisoning. Epidemiological investigation tools are needed to establish surveillance of S. aureus strains in humans, animals and food. In this study, we investigated 145 S. aureus isolates recovered from various animal species, disease conditions, food products and food poisoning events. Multiple Locus Variable Number of Tandem Repeat (VNTR) analysis (MLVA), known to be highly efficient for the genotyping of human S. aureus isolates, was used and shown to be equally well suited for the typing of animal S. aureus isolates. MLVA was improved by using sixteen VNTR loci amplified in two multiplex PCRs and analyzed by capillary electrophoresis ensuring a high throughput and high discriminatory power. The isolates were assigned to twelve known clonal complexes (CCs) and--a few singletons. Half of the test collection belonged to four CCs (CC9, CC97, CC133, CC398) previously described as mostly associated with animals. The remaining eight CCs (CC1, CC5, CC8, CC15, CC25, CC30, CC45, CC51), representing 46% of the animal isolates, are common in humans. Interestingly, isolates responsible for food poisoning show a CC distribution signature typical of human isolates and strikingly different from animal isolates, suggesting a predominantly human origin.Daniel SobralStefan SchwarzDominique BergonierAnne BrisaboisAndrea T FeßlerFlorence B GilbertKristina KadlecBenoit LebeauFabienne Loisy-HamonMichaël TreillesChristine PourcelGilles VergnaudPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e33967 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel Sobral Stefan Schwarz Dominique Bergonier Anne Brisabois Andrea T Feßler Florence B Gilbert Kristina Kadlec Benoit Lebeau Fabienne Loisy-Hamon Michaël Treilles Christine Pourcel Gilles Vergnaud High throughput multiple locus variable number of tandem repeat analysis (MLVA) of Staphylococcus aureus from human, animal and food sources.
description	Staphylococcus aureus is a major human pathogen, a relevant pathogen in veterinary medicine, and a major cause of food poisoning. Epidemiological investigation tools are needed to establish surveillance of S. aureus strains in humans, animals and food. In this study, we investigated 145 S. aureus isolates recovered from various animal species, disease conditions, food products and food poisoning events. Multiple Locus Variable Number of Tandem Repeat (VNTR) analysis (MLVA), known to be highly efficient for the genotyping of human S. aureus isolates, was used and shown to be equally well suited for the typing of animal S. aureus isolates. MLVA was improved by using sixteen VNTR loci amplified in two multiplex PCRs and analyzed by capillary electrophoresis ensuring a high throughput and high discriminatory power. The isolates were assigned to twelve known clonal complexes (CCs) and--a few singletons. Half of the test collection belonged to four CCs (CC9, CC97, CC133, CC398) previously described as mostly associated with animals. The remaining eight CCs (CC1, CC5, CC8, CC15, CC25, CC30, CC45, CC51), representing 46% of the animal isolates, are common in humans. Interestingly, isolates responsible for food poisoning show a CC distribution signature typical of human isolates and strikingly different from animal isolates, suggesting a predominantly human origin.
format	article
author	Daniel Sobral Stefan Schwarz Dominique Bergonier Anne Brisabois Andrea T Feßler Florence B Gilbert Kristina Kadlec Benoit Lebeau Fabienne Loisy-Hamon Michaël Treilles Christine Pourcel Gilles Vergnaud
author_facet	Daniel Sobral Stefan Schwarz Dominique Bergonier Anne Brisabois Andrea T Feßler Florence B Gilbert Kristina Kadlec Benoit Lebeau Fabienne Loisy-Hamon Michaël Treilles Christine Pourcel Gilles Vergnaud
author_sort	Daniel Sobral
title	High throughput multiple locus variable number of tandem repeat analysis (MLVA) of Staphylococcus aureus from human, animal and food sources.
title_short	High throughput multiple locus variable number of tandem repeat analysis (MLVA) of Staphylococcus aureus from human, animal and food sources.
title_full	High throughput multiple locus variable number of tandem repeat analysis (MLVA) of Staphylococcus aureus from human, animal and food sources.
title_fullStr	High throughput multiple locus variable number of tandem repeat analysis (MLVA) of Staphylococcus aureus from human, animal and food sources.
title_full_unstemmed	High throughput multiple locus variable number of tandem repeat analysis (MLVA) of Staphylococcus aureus from human, animal and food sources.
title_sort	high throughput multiple locus variable number of tandem repeat analysis (mlva) of staphylococcus aureus from human, animal and food sources.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/4ef4c825b66340bfa6dd91ed6f63c3c0
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High throughput multiple locus variable number of tandem repeat analysis (MLVA) of Staphylococcus aureus from human, animal and food sources.

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