Occurrence and expression of novel methyl-coenzyme M reductase gene (mcrA) variants in hot spring sediments

Abstract Recent discoveries have shown that the marker gene for anaerobic methane cycling (mcrA) is more widespread in the Archaea than previously thought. However, it remains unclear whether novel mcrA genes associated with the Bathyarchaeota and Verstraetearchaeota are distributed across diverse e...

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Autores principales:	Luke J. McKay, Roland Hatzenpichler, William P. Inskeep, Matthew W. Fields
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4eff383815b640cfb627b7b25a034b7d
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spelling	oai:doaj.org-article:4eff383815b640cfb627b7b25a034b7d2021-12-02T16:06:34ZOccurrence and expression of novel methyl-coenzyme M reductase gene (mcrA) variants in hot spring sediments10.1038/s41598-017-07354-x2045-2322https://doaj.org/article/4eff383815b640cfb627b7b25a034b7d2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07354-xhttps://doaj.org/toc/2045-2322Abstract Recent discoveries have shown that the marker gene for anaerobic methane cycling (mcrA) is more widespread in the Archaea than previously thought. However, it remains unclear whether novel mcrA genes associated with the Bathyarchaeota and Verstraetearchaeota are distributed across diverse environments. We examined two geochemically divergent but putatively methanogenic regions of Yellowstone National Park to investigate whether deeply-rooted archaea possess and express novel mcrA genes in situ. Small-subunit (SSU) rRNA gene analyses indicated that Bathyarchaeota were predominant in seven of ten sediment layers, while the Verstraetearchaeota and Euryarchaeota occurred in lower relative abundance. Targeted amplification of novel mcrA genes suggested that diverse taxa contribute to alkane cycling in geothermal environments. Two deeply-branching mcrA clades related to Bathyarchaeota were identified, while highly abundant verstraetearchaeotal mcrA sequences were also recovered. In addition, detection of SSU rRNA and mcrA transcripts from one hot spring suggested that predominant Bathyarchaeota were also active, and that methane cycling genes are expressed by the Euryarchaeota, Verstraetearchaeota, and an unknown lineage basal to the Bathyarchaeota. These findings greatly expand the diversity of the key marker gene for anaerobic alkane cycling and outline the need for greater understanding of the functional capacity and phylogenetic affiliation of novel mcrA variants.Luke J. McKayRoland HatzenpichlerWilliam P. InskeepMatthew W. FieldsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luke J. McKay Roland Hatzenpichler William P. Inskeep Matthew W. Fields Occurrence and expression of novel methyl-coenzyme M reductase gene (mcrA) variants in hot spring sediments
description	Abstract Recent discoveries have shown that the marker gene for anaerobic methane cycling (mcrA) is more widespread in the Archaea than previously thought. However, it remains unclear whether novel mcrA genes associated with the Bathyarchaeota and Verstraetearchaeota are distributed across diverse environments. We examined two geochemically divergent but putatively methanogenic regions of Yellowstone National Park to investigate whether deeply-rooted archaea possess and express novel mcrA genes in situ. Small-subunit (SSU) rRNA gene analyses indicated that Bathyarchaeota were predominant in seven of ten sediment layers, while the Verstraetearchaeota and Euryarchaeota occurred in lower relative abundance. Targeted amplification of novel mcrA genes suggested that diverse taxa contribute to alkane cycling in geothermal environments. Two deeply-branching mcrA clades related to Bathyarchaeota were identified, while highly abundant verstraetearchaeotal mcrA sequences were also recovered. In addition, detection of SSU rRNA and mcrA transcripts from one hot spring suggested that predominant Bathyarchaeota were also active, and that methane cycling genes are expressed by the Euryarchaeota, Verstraetearchaeota, and an unknown lineage basal to the Bathyarchaeota. These findings greatly expand the diversity of the key marker gene for anaerobic alkane cycling and outline the need for greater understanding of the functional capacity and phylogenetic affiliation of novel mcrA variants.
format	article
author	Luke J. McKay Roland Hatzenpichler William P. Inskeep Matthew W. Fields
author_facet	Luke J. McKay Roland Hatzenpichler William P. Inskeep Matthew W. Fields
author_sort	Luke J. McKay
title	Occurrence and expression of novel methyl-coenzyme M reductase gene (mcrA) variants in hot spring sediments
title_short	Occurrence and expression of novel methyl-coenzyme M reductase gene (mcrA) variants in hot spring sediments
title_full	Occurrence and expression of novel methyl-coenzyme M reductase gene (mcrA) variants in hot spring sediments
title_fullStr	Occurrence and expression of novel methyl-coenzyme M reductase gene (mcrA) variants in hot spring sediments
title_full_unstemmed	Occurrence and expression of novel methyl-coenzyme M reductase gene (mcrA) variants in hot spring sediments
title_sort	occurrence and expression of novel methyl-coenzyme m reductase gene (mcra) variants in hot spring sediments
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4eff383815b640cfb627b7b25a034b7d
work_keys_str_mv	AT lukejmckay occurrenceandexpressionofnovelmethylcoenzymemreductasegenemcravariantsinhotspringsediments AT rolandhatzenpichler occurrenceandexpressionofnovelmethylcoenzymemreductasegenemcravariantsinhotspringsediments AT williampinskeep occurrenceandexpressionofnovelmethylcoenzymemreductasegenemcravariantsinhotspringsediments AT matthewwfields occurrenceandexpressionofnovelmethylcoenzymemreductasegenemcravariantsinhotspringsediments
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Occurrence and expression of novel methyl-coenzyme M reductase gene (mcrA) variants in hot spring sediments

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