EFFECTS OF BACTERIAL LIGANDS OF PATTERN-RECOGNIZING RECEPTORS (PRR) ON MONOCYTE-LIKE THP-1 CELLS UPON THEIR TRANSENDOTHELIAL MIGRATION

Abstract. The aim of study was to compare the influence of lipopolysaccharide (LPS) component from Gram-negative bacteria (E. coli 055:B5), and a lysate of Gram-positive bacterium (Streptococcus pyogenes, type M1, strain 40/58) upon transendothelial migration rates of monocyte-like cells (THP-1 stra...

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Autores principales:	E. P. Starikova, D. I. Sokolov, L. A. Burova, S. A. Sel’kov, I. S. Freidlin
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	transendothelial migration monocytes cytokines chemokines prr rantes mcp-1 il-8 ip-10 tnfα il-1β il-6 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/4f0c7fd8e9f745e1a8d494f1ada5c91f
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spelling	oai:doaj.org-article:4f0c7fd8e9f745e1a8d494f1ada5c91f2021-11-18T08:03:39ZEFFECTS OF BACTERIAL LIGANDS OF PATTERN-RECOGNIZING RECEPTORS (PRR) ON MONOCYTE-LIKE THP-1 CELLS UPON THEIR TRANSENDOTHELIAL MIGRATION1563-06252313-741X10.15789/1563-0625-2008-6-571-576https://doaj.org/article/4f0c7fd8e9f745e1a8d494f1ada5c91f2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/271https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. The aim of study was to compare the influence of lipopolysaccharide (LPS) component from Gram-negative bacteria (E. coli 055:B5), and a lysate of Gram-positive bacterium (Streptococcus pyogenes, type M1, strain 40/58) upon transendothelial migration rates of monocyte-like cells (THP-1 strain). Both LPS and lysate of Streptococcus pyogenes acted as chemoattractants for THP-1 cells. he studied components of Streptococcus pyogenes proved to be more active stimulants of transendothelial THP-1 cell migration, than LPS from E. coli. During spontaneous transmigration of THP-1 cells through a monolayer of endothelial cells, augmented levels of chemokines (RANTES, MCP-1, IL-8, IP-10) were noticed, that were more pronounced in presence of LPS. Upon spontaneous transmigration of THP-1 cells through endothelial monolayer, the levels of proinflammatory cytokines (TNFα, IL-1β and IL-6) in cultural medium were found to be rather low. The transmigration-associated secretion of these cytokines increased in presence of LPS and Streptococcus pyogenes lysate. Incubation with these bacterial constituents did increase cytokine levels both in monoculture of THP-1 cells and in transmigration model. Our results suggest that the levels of THP-1 transendothelial migration depend mainly on activation of monocyte-like cells influenced by PRR-ligands from Streptococcus pyogenes lysate. (Med. Immunol., vol. 10, N 6, pp 571-576).E. P. StarikovaD. I. SokolovL. A. BurovaS. A. Sel’kovI. S. FreidlinSPb RAACIarticletransendothelial migrationmonocytescytokineschemokinesprrrantesmcp-1il-8ip-10tnfαil-1βil-6Immunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 10, Iss 6, Pp 571-576 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	transendothelial migration monocytes cytokines chemokines prr rantes mcp-1 il-8 ip-10 tnfα il-1β il-6 Immunologic diseases. Allergy RC581-607
spellingShingle	transendothelial migration monocytes cytokines chemokines prr rantes mcp-1 il-8 ip-10 tnfα il-1β il-6 Immunologic diseases. Allergy RC581-607 E. P. Starikova D. I. Sokolov L. A. Burova S. A. Sel’kov I. S. Freidlin EFFECTS OF BACTERIAL LIGANDS OF PATTERN-RECOGNIZING RECEPTORS (PRR) ON MONOCYTE-LIKE THP-1 CELLS UPON THEIR TRANSENDOTHELIAL MIGRATION
description	Abstract. The aim of study was to compare the influence of lipopolysaccharide (LPS) component from Gram-negative bacteria (E. coli 055:B5), and a lysate of Gram-positive bacterium (Streptococcus pyogenes, type M1, strain 40/58) upon transendothelial migration rates of monocyte-like cells (THP-1 strain). Both LPS and lysate of Streptococcus pyogenes acted as chemoattractants for THP-1 cells. he studied components of Streptococcus pyogenes proved to be more active stimulants of transendothelial THP-1 cell migration, than LPS from E. coli. During spontaneous transmigration of THP-1 cells through a monolayer of endothelial cells, augmented levels of chemokines (RANTES, MCP-1, IL-8, IP-10) were noticed, that were more pronounced in presence of LPS. Upon spontaneous transmigration of THP-1 cells through endothelial monolayer, the levels of proinflammatory cytokines (TNFα, IL-1β and IL-6) in cultural medium were found to be rather low. The transmigration-associated secretion of these cytokines increased in presence of LPS and Streptococcus pyogenes lysate. Incubation with these bacterial constituents did increase cytokine levels both in monoculture of THP-1 cells and in transmigration model. Our results suggest that the levels of THP-1 transendothelial migration depend mainly on activation of monocyte-like cells influenced by PRR-ligands from Streptococcus pyogenes lysate. (Med. Immunol., vol. 10, N 6, pp 571-576).
format	article
author	E. P. Starikova D. I. Sokolov L. A. Burova S. A. Sel’kov I. S. Freidlin
author_facet	E. P. Starikova D. I. Sokolov L. A. Burova S. A. Sel’kov I. S. Freidlin
author_sort	E. P. Starikova
title	EFFECTS OF BACTERIAL LIGANDS OF PATTERN-RECOGNIZING RECEPTORS (PRR) ON MONOCYTE-LIKE THP-1 CELLS UPON THEIR TRANSENDOTHELIAL MIGRATION
title_short	EFFECTS OF BACTERIAL LIGANDS OF PATTERN-RECOGNIZING RECEPTORS (PRR) ON MONOCYTE-LIKE THP-1 CELLS UPON THEIR TRANSENDOTHELIAL MIGRATION
title_full	EFFECTS OF BACTERIAL LIGANDS OF PATTERN-RECOGNIZING RECEPTORS (PRR) ON MONOCYTE-LIKE THP-1 CELLS UPON THEIR TRANSENDOTHELIAL MIGRATION
title_fullStr	EFFECTS OF BACTERIAL LIGANDS OF PATTERN-RECOGNIZING RECEPTORS (PRR) ON MONOCYTE-LIKE THP-1 CELLS UPON THEIR TRANSENDOTHELIAL MIGRATION
title_full_unstemmed	EFFECTS OF BACTERIAL LIGANDS OF PATTERN-RECOGNIZING RECEPTORS (PRR) ON MONOCYTE-LIKE THP-1 CELLS UPON THEIR TRANSENDOTHELIAL MIGRATION
title_sort	effects of bacterial ligands of pattern-recognizing receptors (prr) on monocyte-like thp-1 cells upon their transendothelial migration
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/4f0c7fd8e9f745e1a8d494f1ada5c91f
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_version_	1718422543366356992

EFFECTS OF BACTERIAL LIGANDS OF PATTERN-RECOGNIZING RECEPTORS (PRR) ON MONOCYTE-LIKE THP-1 CELLS UPON THEIR TRANSENDOTHELIAL MIGRATION

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