Genome-wide identification and expression pattern analysis of the ribonuclease T2 family in Eucommia ulmoides

Abstract The 2′,3′-cycling ribonuclease (RNase) genes are catalysts of RNA cleavage and include the RNase T2 gene family. RNase T2 genes perform important roles in plants and have been conserved in the genome of eukaryotic organisms. In this study we identified 21 EURNS genes in Eucommia ulmoides Ol...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jun Qing, Qingxin Du, Yide Meng, Panfeng Liu, Hongyan Du, Lu Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4f1838eb70c5485296ec3bcc9b80dd5f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:4f1838eb70c5485296ec3bcc9b80dd5f
record_format	dspace
spelling	oai:doaj.org-article:4f1838eb70c5485296ec3bcc9b80dd5f2021-12-02T17:04:07ZGenome-wide identification and expression pattern analysis of the ribonuclease T2 family in Eucommia ulmoides10.1038/s41598-021-86337-52045-2322https://doaj.org/article/4f1838eb70c5485296ec3bcc9b80dd5f2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86337-5https://doaj.org/toc/2045-2322Abstract The 2′,3′-cycling ribonuclease (RNase) genes are catalysts of RNA cleavage and include the RNase T2 gene family. RNase T2 genes perform important roles in plants and have been conserved in the genome of eukaryotic organisms. In this study we identified 21 EURNS genes in Eucommia ulmoides Oliver (E. ulmoides) and analyzed their structure, chromosomal location, phylogenetic tree, gene duplication, stress-related cis-elements, and expression patterns in different tissues. The length of 21 predicted EURNS proteins ranged from 143 to 374 amino acids (aa), their molecular weight (MW) ranged from 16.21 to 42.38 kDa, and their isoelectric point (PI) value ranged from 5.08 to 9.09. Two classifications (class I and class III) were obtained from the conserved domains analysis and phylogenetic tree. EURNS proteins contained a total of 15 motifs. Motif 1, motif 2, motif 3, and motif 7 were distributed in multiple sequences and were similar to the conserved domain of RNase T2. EURNS genes with similar structure and the predicted EURNS proteins with conserved motif compositions are in the same group in the phylogenetic tree. The results of RT-PCR and transcription data showed that EURNS genes have tissue-specific expression and exhibited obvious trends in different developmental stages. Gene duplication analysis results indicated that segment duplication may be the dominant duplication mode in this gene family. This study provides a theoretical basis for research on the RNase T2 gene family and lays a foundation for the further study of EURNS genes.Jun QingQingxin DuYide MengPanfeng LiuHongyan DuLu WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jun Qing Qingxin Du Yide Meng Panfeng Liu Hongyan Du Lu Wang Genome-wide identification and expression pattern analysis of the ribonuclease T2 family in Eucommia ulmoides
description	Abstract The 2′,3′-cycling ribonuclease (RNase) genes are catalysts of RNA cleavage and include the RNase T2 gene family. RNase T2 genes perform important roles in plants and have been conserved in the genome of eukaryotic organisms. In this study we identified 21 EURNS genes in Eucommia ulmoides Oliver (E. ulmoides) and analyzed their structure, chromosomal location, phylogenetic tree, gene duplication, stress-related cis-elements, and expression patterns in different tissues. The length of 21 predicted EURNS proteins ranged from 143 to 374 amino acids (aa), their molecular weight (MW) ranged from 16.21 to 42.38 kDa, and their isoelectric point (PI) value ranged from 5.08 to 9.09. Two classifications (class I and class III) were obtained from the conserved domains analysis and phylogenetic tree. EURNS proteins contained a total of 15 motifs. Motif 1, motif 2, motif 3, and motif 7 were distributed in multiple sequences and were similar to the conserved domain of RNase T2. EURNS genes with similar structure and the predicted EURNS proteins with conserved motif compositions are in the same group in the phylogenetic tree. The results of RT-PCR and transcription data showed that EURNS genes have tissue-specific expression and exhibited obvious trends in different developmental stages. Gene duplication analysis results indicated that segment duplication may be the dominant duplication mode in this gene family. This study provides a theoretical basis for research on the RNase T2 gene family and lays a foundation for the further study of EURNS genes.
format	article
author	Jun Qing Qingxin Du Yide Meng Panfeng Liu Hongyan Du Lu Wang
author_facet	Jun Qing Qingxin Du Yide Meng Panfeng Liu Hongyan Du Lu Wang
author_sort	Jun Qing
title	Genome-wide identification and expression pattern analysis of the ribonuclease T2 family in Eucommia ulmoides
title_short	Genome-wide identification and expression pattern analysis of the ribonuclease T2 family in Eucommia ulmoides
title_full	Genome-wide identification and expression pattern analysis of the ribonuclease T2 family in Eucommia ulmoides
title_fullStr	Genome-wide identification and expression pattern analysis of the ribonuclease T2 family in Eucommia ulmoides
title_full_unstemmed	Genome-wide identification and expression pattern analysis of the ribonuclease T2 family in Eucommia ulmoides
title_sort	genome-wide identification and expression pattern analysis of the ribonuclease t2 family in eucommia ulmoides
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4f1838eb70c5485296ec3bcc9b80dd5f
work_keys_str_mv	AT junqing genomewideidentificationandexpressionpatternanalysisoftheribonucleaset2familyineucommiaulmoides AT qingxindu genomewideidentificationandexpressionpatternanalysisoftheribonucleaset2familyineucommiaulmoides AT yidemeng genomewideidentificationandexpressionpatternanalysisoftheribonucleaset2familyineucommiaulmoides AT panfengliu genomewideidentificationandexpressionpatternanalysisoftheribonucleaset2familyineucommiaulmoides AT hongyandu genomewideidentificationandexpressionpatternanalysisoftheribonucleaset2familyineucommiaulmoides AT luwang genomewideidentificationandexpressionpatternanalysisoftheribonucleaset2familyineucommiaulmoides
_version_	1718381847435542528

Genome-wide identification and expression pattern analysis of the ribonuclease T2 family in Eucommia ulmoides

Ejemplares similares