Impact of SARS-CoV-2 infection on the recovery of peripheral blood mononuclear cells by density gradient

Abstract SARS-CoV-2 virus infection is responsible for coronavirus disease (COVID-19), which is characterised by a hyperinflammatory response that plays a major role in determining the respiratory and immune-mediated complications of this condition. While isolating peripheral blood mononuclear cells...

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Autores principales:	Maria D. I. Manunta, Giuseppe Lamorte, Francesca Ferrari, Elena Trombetta, Mario Tirone, Cristiana Bianco, Alessandra Cattaneo, Luigi Santoro, Guido Baselli, Manuela Brasca, Mahnoosh Ostadreza, Elisa Erba, Andrea Gori, Alessandra Bandera, Laura Porretti, Luca V. C. Valenti, Daniele Prati
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4f22970303e04a0588398de3ed1899c9
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spelling	oai:doaj.org-article:4f22970303e04a0588398de3ed1899c92021-12-02T15:53:46ZImpact of SARS-CoV-2 infection on the recovery of peripheral blood mononuclear cells by density gradient10.1038/s41598-021-83950-22045-2322https://doaj.org/article/4f22970303e04a0588398de3ed1899c92021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83950-2https://doaj.org/toc/2045-2322Abstract SARS-CoV-2 virus infection is responsible for coronavirus disease (COVID-19), which is characterised by a hyperinflammatory response that plays a major role in determining the respiratory and immune-mediated complications of this condition. While isolating peripheral blood mononuclear cells (PBMCs) from whole blood of COVID-19 patients by density gradient centrifugation, we noticed some changes in the floating properties and in the sedimentation of the cells on density medium. Investigating this further, we found that in early phase COVID-19 patients, characterised by reduced circulating lymphocytes and monocytes, the PBMC fraction contained surprisingly high levels of neutrophils. Furthermore, the neutrophil population exhibited alterations in the cell size and in the internal complexity, consistent with the presence of low density neutrophils (LDNs) and immature forms, which may explain the shift seen in the floating abilities and that may be predictive of the severity of the disease. The percentage of this subset of neutrophils found in the PBMC band was rather spread (35.4 ± 27.2%, with a median 28.8% and IQR 11.6–56.1, Welch’s t-test early phase COVID-19 versus blood donor healthy controls P < 0.0001). Results confirm the presence of an increased number of LDNs in patients with early stage COVID-19, which correlates with disease severity and may be recovered by centrifugation on a density gradient together with PBMCs.Maria D. I. ManuntaGiuseppe LamorteFrancesca FerrariElena TrombettaMario TironeCristiana BiancoAlessandra CattaneoLuigi SantoroGuido BaselliManuela BrascaMahnoosh OstadrezaElisa ErbaAndrea GoriAlessandra BanderaLaura PorrettiLuca V. C. ValentiDaniele PratiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria D. I. Manunta Giuseppe Lamorte Francesca Ferrari Elena Trombetta Mario Tirone Cristiana Bianco Alessandra Cattaneo Luigi Santoro Guido Baselli Manuela Brasca Mahnoosh Ostadreza Elisa Erba Andrea Gori Alessandra Bandera Laura Porretti Luca V. C. Valenti Daniele Prati Impact of SARS-CoV-2 infection on the recovery of peripheral blood mononuclear cells by density gradient
description	Abstract SARS-CoV-2 virus infection is responsible for coronavirus disease (COVID-19), which is characterised by a hyperinflammatory response that plays a major role in determining the respiratory and immune-mediated complications of this condition. While isolating peripheral blood mononuclear cells (PBMCs) from whole blood of COVID-19 patients by density gradient centrifugation, we noticed some changes in the floating properties and in the sedimentation of the cells on density medium. Investigating this further, we found that in early phase COVID-19 patients, characterised by reduced circulating lymphocytes and monocytes, the PBMC fraction contained surprisingly high levels of neutrophils. Furthermore, the neutrophil population exhibited alterations in the cell size and in the internal complexity, consistent with the presence of low density neutrophils (LDNs) and immature forms, which may explain the shift seen in the floating abilities and that may be predictive of the severity of the disease. The percentage of this subset of neutrophils found in the PBMC band was rather spread (35.4 ± 27.2%, with a median 28.8% and IQR 11.6–56.1, Welch’s t-test early phase COVID-19 versus blood donor healthy controls P < 0.0001). Results confirm the presence of an increased number of LDNs in patients with early stage COVID-19, which correlates with disease severity and may be recovered by centrifugation on a density gradient together with PBMCs.
format	article
author	Maria D. I. Manunta Giuseppe Lamorte Francesca Ferrari Elena Trombetta Mario Tirone Cristiana Bianco Alessandra Cattaneo Luigi Santoro Guido Baselli Manuela Brasca Mahnoosh Ostadreza Elisa Erba Andrea Gori Alessandra Bandera Laura Porretti Luca V. C. Valenti Daniele Prati
author_facet	Maria D. I. Manunta Giuseppe Lamorte Francesca Ferrari Elena Trombetta Mario Tirone Cristiana Bianco Alessandra Cattaneo Luigi Santoro Guido Baselli Manuela Brasca Mahnoosh Ostadreza Elisa Erba Andrea Gori Alessandra Bandera Laura Porretti Luca V. C. Valenti Daniele Prati
author_sort	Maria D. I. Manunta
title	Impact of SARS-CoV-2 infection on the recovery of peripheral blood mononuclear cells by density gradient
title_short	Impact of SARS-CoV-2 infection on the recovery of peripheral blood mononuclear cells by density gradient
title_full	Impact of SARS-CoV-2 infection on the recovery of peripheral blood mononuclear cells by density gradient
title_fullStr	Impact of SARS-CoV-2 infection on the recovery of peripheral blood mononuclear cells by density gradient
title_full_unstemmed	Impact of SARS-CoV-2 infection on the recovery of peripheral blood mononuclear cells by density gradient
title_sort	impact of sars-cov-2 infection on the recovery of peripheral blood mononuclear cells by density gradient
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4f22970303e04a0588398de3ed1899c9
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Impact of SARS-CoV-2 infection on the recovery of peripheral blood mononuclear cells by density gradient

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