Effects of Low Doses of L-Carnitine Tartrate and Lipid Multi-Particulate Formulated Creatine Monohydrate on Muscle Protein Synthesis in Myoblasts and Bioavailability in Humans and Rodents
The primary objective of this study was to investigate the potential synergy between low doses of L-carnitine tartrate and creatine monohydrate to induce muscle protein synthesis and anabolic pathway activation in primary human myoblasts. In addition, the effects of Lipid multi-particulates (LMP) fo...
Guardado en:
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/4f2b5f1bf8ef479d904cccc7ff606748 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:4f2b5f1bf8ef479d904cccc7ff606748 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:4f2b5f1bf8ef479d904cccc7ff6067482021-11-25T18:35:48ZEffects of Low Doses of L-Carnitine Tartrate and Lipid Multi-Particulate Formulated Creatine Monohydrate on Muscle Protein Synthesis in Myoblasts and Bioavailability in Humans and Rodents10.3390/nu131139852072-6643https://doaj.org/article/4f2b5f1bf8ef479d904cccc7ff6067482021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6643/13/11/3985https://doaj.org/toc/2072-6643The primary objective of this study was to investigate the potential synergy between low doses of L-carnitine tartrate and creatine monohydrate to induce muscle protein synthesis and anabolic pathway activation in primary human myoblasts. In addition, the effects of Lipid multi-particulates (LMP) formulation on creatine stability and bioavailability were assessed in rodents and healthy human subjects. When used individually, L-carnitine tartrate at 50 µM and creatine monohydrate at 0.5 µM did not affect myoblast protein synthesis and signaling. However, when combined, they led to a significant increase in protein synthesis. Increased AKT and RPS6 phosphorylation were observed with 50 µM L-carnitine tartrate 5 µM creatine in combination in primary human myoblasts. When Wistar rats were administered creatine with LMP formulation at either 21 or 51 mg/kg, bioavailability was increased by 27% based on the increase in the area under the curve (AUC) at a 51 mg/kg dose compared to without LMP formulation. Tmax and Cmax were unchanged. Finally, in human subjects, a combination of LMP formulated L-carnitine at 500 mg (from L-carnitine tartrate) with LMP formulated creatine at 100, 200, or 500 mg revealed a significant and dose-dependent increase in plasma creatine concentrations. Serum total L-carnitine levels rose in a similar manner in the three combinations. These results suggest that a combination of low doses of L-carnitine tartrate and creatine monohydrate may lead to a significant and synergistic enhancement of muscle protein synthesis and activation of anabolic signaling. In addition, the LMP formulation of creatine improved its bioavailability. L-carnitine at 500 mg and LMP-formulated creatine at 200 or 500 mg may be useful for future clinical trials to evaluate the effects on muscle protein synthesis.Roger A. FieldingDonato RivasGregory J. GrosickiYassine EzzyatLisa CegliaLori Lyn PriceCemal OrhanKazim SahinKelli FowlerTyler WhiteShane DurkeeKatja KritschAouatef BellamineMDPI AGarticlemusclecarnitinecreatineprotein synthesisNutrition. Foods and food supplyTX341-641ENNutrients, Vol 13, Iss 3985, p 3985 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
muscle carnitine creatine protein synthesis Nutrition. Foods and food supply TX341-641 |
| spellingShingle |
muscle carnitine creatine protein synthesis Nutrition. Foods and food supply TX341-641 Roger A. Fielding Donato Rivas Gregory J. Grosicki Yassine Ezzyat Lisa Ceglia Lori Lyn Price Cemal Orhan Kazim Sahin Kelli Fowler Tyler White Shane Durkee Katja Kritsch Aouatef Bellamine Effects of Low Doses of L-Carnitine Tartrate and Lipid Multi-Particulate Formulated Creatine Monohydrate on Muscle Protein Synthesis in Myoblasts and Bioavailability in Humans and Rodents |
| description |
The primary objective of this study was to investigate the potential synergy between low doses of L-carnitine tartrate and creatine monohydrate to induce muscle protein synthesis and anabolic pathway activation in primary human myoblasts. In addition, the effects of Lipid multi-particulates (LMP) formulation on creatine stability and bioavailability were assessed in rodents and healthy human subjects. When used individually, L-carnitine tartrate at 50 µM and creatine monohydrate at 0.5 µM did not affect myoblast protein synthesis and signaling. However, when combined, they led to a significant increase in protein synthesis. Increased AKT and RPS6 phosphorylation were observed with 50 µM L-carnitine tartrate 5 µM creatine in combination in primary human myoblasts. When Wistar rats were administered creatine with LMP formulation at either 21 or 51 mg/kg, bioavailability was increased by 27% based on the increase in the area under the curve (AUC) at a 51 mg/kg dose compared to without LMP formulation. Tmax and Cmax were unchanged. Finally, in human subjects, a combination of LMP formulated L-carnitine at 500 mg (from L-carnitine tartrate) with LMP formulated creatine at 100, 200, or 500 mg revealed a significant and dose-dependent increase in plasma creatine concentrations. Serum total L-carnitine levels rose in a similar manner in the three combinations. These results suggest that a combination of low doses of L-carnitine tartrate and creatine monohydrate may lead to a significant and synergistic enhancement of muscle protein synthesis and activation of anabolic signaling. In addition, the LMP formulation of creatine improved its bioavailability. L-carnitine at 500 mg and LMP-formulated creatine at 200 or 500 mg may be useful for future clinical trials to evaluate the effects on muscle protein synthesis. |
| format |
article |
| author |
Roger A. Fielding Donato Rivas Gregory J. Grosicki Yassine Ezzyat Lisa Ceglia Lori Lyn Price Cemal Orhan Kazim Sahin Kelli Fowler Tyler White Shane Durkee Katja Kritsch Aouatef Bellamine |
| author_facet |
Roger A. Fielding Donato Rivas Gregory J. Grosicki Yassine Ezzyat Lisa Ceglia Lori Lyn Price Cemal Orhan Kazim Sahin Kelli Fowler Tyler White Shane Durkee Katja Kritsch Aouatef Bellamine |
| author_sort |
Roger A. Fielding |
| title |
Effects of Low Doses of L-Carnitine Tartrate and Lipid Multi-Particulate Formulated Creatine Monohydrate on Muscle Protein Synthesis in Myoblasts and Bioavailability in Humans and Rodents |
| title_short |
Effects of Low Doses of L-Carnitine Tartrate and Lipid Multi-Particulate Formulated Creatine Monohydrate on Muscle Protein Synthesis in Myoblasts and Bioavailability in Humans and Rodents |
| title_full |
Effects of Low Doses of L-Carnitine Tartrate and Lipid Multi-Particulate Formulated Creatine Monohydrate on Muscle Protein Synthesis in Myoblasts and Bioavailability in Humans and Rodents |
| title_fullStr |
Effects of Low Doses of L-Carnitine Tartrate and Lipid Multi-Particulate Formulated Creatine Monohydrate on Muscle Protein Synthesis in Myoblasts and Bioavailability in Humans and Rodents |
| title_full_unstemmed |
Effects of Low Doses of L-Carnitine Tartrate and Lipid Multi-Particulate Formulated Creatine Monohydrate on Muscle Protein Synthesis in Myoblasts and Bioavailability in Humans and Rodents |
| title_sort |
effects of low doses of l-carnitine tartrate and lipid multi-particulate formulated creatine monohydrate on muscle protein synthesis in myoblasts and bioavailability in humans and rodents |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/4f2b5f1bf8ef479d904cccc7ff606748 |
| work_keys_str_mv |
AT rogerafielding effectsoflowdosesoflcarnitinetartrateandlipidmultiparticulateformulatedcreatinemonohydrateonmuscleproteinsynthesisinmyoblastsandbioavailabilityinhumansandrodents AT donatorivas effectsoflowdosesoflcarnitinetartrateandlipidmultiparticulateformulatedcreatinemonohydrateonmuscleproteinsynthesisinmyoblastsandbioavailabilityinhumansandrodents AT gregoryjgrosicki effectsoflowdosesoflcarnitinetartrateandlipidmultiparticulateformulatedcreatinemonohydrateonmuscleproteinsynthesisinmyoblastsandbioavailabilityinhumansandrodents AT yassineezzyat effectsoflowdosesoflcarnitinetartrateandlipidmultiparticulateformulatedcreatinemonohydrateonmuscleproteinsynthesisinmyoblastsandbioavailabilityinhumansandrodents AT lisaceglia effectsoflowdosesoflcarnitinetartrateandlipidmultiparticulateformulatedcreatinemonohydrateonmuscleproteinsynthesisinmyoblastsandbioavailabilityinhumansandrodents AT lorilynprice effectsoflowdosesoflcarnitinetartrateandlipidmultiparticulateformulatedcreatinemonohydrateonmuscleproteinsynthesisinmyoblastsandbioavailabilityinhumansandrodents AT cemalorhan effectsoflowdosesoflcarnitinetartrateandlipidmultiparticulateformulatedcreatinemonohydrateonmuscleproteinsynthesisinmyoblastsandbioavailabilityinhumansandrodents AT kazimsahin effectsoflowdosesoflcarnitinetartrateandlipidmultiparticulateformulatedcreatinemonohydrateonmuscleproteinsynthesisinmyoblastsandbioavailabilityinhumansandrodents AT kellifowler effectsoflowdosesoflcarnitinetartrateandlipidmultiparticulateformulatedcreatinemonohydrateonmuscleproteinsynthesisinmyoblastsandbioavailabilityinhumansandrodents AT tylerwhite effectsoflowdosesoflcarnitinetartrateandlipidmultiparticulateformulatedcreatinemonohydrateonmuscleproteinsynthesisinmyoblastsandbioavailabilityinhumansandrodents AT shanedurkee effectsoflowdosesoflcarnitinetartrateandlipidmultiparticulateformulatedcreatinemonohydrateonmuscleproteinsynthesisinmyoblastsandbioavailabilityinhumansandrodents AT katjakritsch effectsoflowdosesoflcarnitinetartrateandlipidmultiparticulateformulatedcreatinemonohydrateonmuscleproteinsynthesisinmyoblastsandbioavailabilityinhumansandrodents AT aouatefbellamine effectsoflowdosesoflcarnitinetartrateandlipidmultiparticulateformulatedcreatinemonohydrateonmuscleproteinsynthesisinmyoblastsandbioavailabilityinhumansandrodents |
| _version_ |
1718410952378941440 |