MEMS enabled miniaturized light-sheet microscopy with all optical control

Abstract We have designed and implemented a compact, cost-efficient miniaturised light-sheet microscopy system based on optical microelectromechanical systems scanners and tunable lenses. The system occupies a footprint of 20 × 28 × 13 cm3 and combines off-the-shelf optics and optomechanics with 3D-...

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Autores principales:	Spyridon Bakas, Deepak Uttamchandani, Hiroshi Toshiyoshi, Ralf Bauer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4f2b7fe0196a440886cf341b39da2a8f
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spelling	oai:doaj.org-article:4f2b7fe0196a440886cf341b39da2a8f2021-12-02T18:33:58ZMEMS enabled miniaturized light-sheet microscopy with all optical control10.1038/s41598-021-93454-82045-2322https://doaj.org/article/4f2b7fe0196a440886cf341b39da2a8f2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93454-8https://doaj.org/toc/2045-2322Abstract We have designed and implemented a compact, cost-efficient miniaturised light-sheet microscopy system based on optical microelectromechanical systems scanners and tunable lenses. The system occupies a footprint of 20 × 28 × 13 cm3 and combines off-the-shelf optics and optomechanics with 3D-printed structural and optical elements, and an economically costed objective lens, excitation laser and camera. All-optical volume scanning enables imaging of 435 × 232 × 60 µm3 volumes with 0.25 vps (volumes per second) and minimum lateral and axial resolution of 1.0 µm and 3.8 µm respectively. An open-top geometry allows imaging of samples on flat bottomed holders, allowing integration with microfluidic devices, multi-well plates and slide mounted samples, with applications envisaged in biomedical research and pre-clinical settings.Spyridon BakasDeepak UttamchandaniHiroshi ToshiyoshiRalf BauerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Spyridon Bakas Deepak Uttamchandani Hiroshi Toshiyoshi Ralf Bauer MEMS enabled miniaturized light-sheet microscopy with all optical control
description	Abstract We have designed and implemented a compact, cost-efficient miniaturised light-sheet microscopy system based on optical microelectromechanical systems scanners and tunable lenses. The system occupies a footprint of 20 × 28 × 13 cm3 and combines off-the-shelf optics and optomechanics with 3D-printed structural and optical elements, and an economically costed objective lens, excitation laser and camera. All-optical volume scanning enables imaging of 435 × 232 × 60 µm3 volumes with 0.25 vps (volumes per second) and minimum lateral and axial resolution of 1.0 µm and 3.8 µm respectively. An open-top geometry allows imaging of samples on flat bottomed holders, allowing integration with microfluidic devices, multi-well plates and slide mounted samples, with applications envisaged in biomedical research and pre-clinical settings.
format	article
author	Spyridon Bakas Deepak Uttamchandani Hiroshi Toshiyoshi Ralf Bauer
author_facet	Spyridon Bakas Deepak Uttamchandani Hiroshi Toshiyoshi Ralf Bauer
author_sort	Spyridon Bakas
title	MEMS enabled miniaturized light-sheet microscopy with all optical control
title_short	MEMS enabled miniaturized light-sheet microscopy with all optical control
title_full	MEMS enabled miniaturized light-sheet microscopy with all optical control
title_fullStr	MEMS enabled miniaturized light-sheet microscopy with all optical control
title_full_unstemmed	MEMS enabled miniaturized light-sheet microscopy with all optical control
title_sort	mems enabled miniaturized light-sheet microscopy with all optical control
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4f2b7fe0196a440886cf341b39da2a8f
work_keys_str_mv	AT spyridonbakas memsenabledminiaturizedlightsheetmicroscopywithallopticalcontrol AT deepakuttamchandani memsenabledminiaturizedlightsheetmicroscopywithallopticalcontrol AT hiroshitoshiyoshi memsenabledminiaturizedlightsheetmicroscopywithallopticalcontrol AT ralfbauer memsenabledminiaturizedlightsheetmicroscopywithallopticalcontrol
_version_	1718377918136057856

MEMS enabled miniaturized light-sheet microscopy with all optical control

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