Characterization of bacterial community changes and antibiotic resistance genes in lamb manure of different incidence

Abstract Bacterial enteritis is the most important disease in lamb for breeding greatly affects the growth of animals. Changes in the community of intestinal flora can cause the disorder of the colonic environment induced diarrhea. This study aimed to investigate the relationship between the inciden...

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Autores principales:	Ling-cong Kong, Bo Wang, Yi-ming Wang, Ren-ge Hu, AtiahLujia Atiewin, Duo Gao, Yun-hang Gao, Hong-xia Ma
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4f2ea9bb32114f78b7eedc99540c895f
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spelling	oai:doaj.org-article:4f2ea9bb32114f78b7eedc99540c895f2021-12-02T15:09:20ZCharacterization of bacterial community changes and antibiotic resistance genes in lamb manure of different incidence10.1038/s41598-019-46604-y2045-2322https://doaj.org/article/4f2ea9bb32114f78b7eedc99540c895f2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-46604-yhttps://doaj.org/toc/2045-2322Abstract Bacterial enteritis is the most important disease in lamb for breeding greatly affects the growth of animals. Changes in the community of intestinal flora can cause the disorder of the colonic environment induced diarrhea. This study aimed to investigate the relationship between the incidence of bacterial enteritis and the number of intestinal microbiome, then the prevalence of drug-resistant genes was detected. Fecal samples were collected at five fattening sheep farms with different incidence of bacterial enteritis, pathogenic bacteria were isolated and identified, drug sensitivity tests were performed. Then, changes in number and structure of intestinal flora were compared by 16S rDNA V3-V4 region high-throughput sequencing, and the ARGs were detected using high-throughput real-time PCR. Our results revealed that the microbial communities were positively correlated with the incidence of bacterial enteritis in different farms. Bacterial communities were higher in YJ (with highest incidence of diarrhea) than any other farms. However, the ARGs seemed not to be more affected by the incidence of bacterial enteritis, but one of the significant findings to emerge from this study is that MCR-1 and NDM are detected in manure. This study has provided an insight of the changes occurring in intestinal flora and AGRs in fattening sheep farms with diverse incidence of bacterial enteritis.Ling-cong KongBo WangYi-ming WangRen-ge HuAtiahLujia AtiewinDuo GaoYun-hang GaoHong-xia MaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-7 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ling-cong Kong Bo Wang Yi-ming Wang Ren-ge Hu AtiahLujia Atiewin Duo Gao Yun-hang Gao Hong-xia Ma Characterization of bacterial community changes and antibiotic resistance genes in lamb manure of different incidence
description	Abstract Bacterial enteritis is the most important disease in lamb for breeding greatly affects the growth of animals. Changes in the community of intestinal flora can cause the disorder of the colonic environment induced diarrhea. This study aimed to investigate the relationship between the incidence of bacterial enteritis and the number of intestinal microbiome, then the prevalence of drug-resistant genes was detected. Fecal samples were collected at five fattening sheep farms with different incidence of bacterial enteritis, pathogenic bacteria were isolated and identified, drug sensitivity tests were performed. Then, changes in number and structure of intestinal flora were compared by 16S rDNA V3-V4 region high-throughput sequencing, and the ARGs were detected using high-throughput real-time PCR. Our results revealed that the microbial communities were positively correlated with the incidence of bacterial enteritis in different farms. Bacterial communities were higher in YJ (with highest incidence of diarrhea) than any other farms. However, the ARGs seemed not to be more affected by the incidence of bacterial enteritis, but one of the significant findings to emerge from this study is that MCR-1 and NDM are detected in manure. This study has provided an insight of the changes occurring in intestinal flora and AGRs in fattening sheep farms with diverse incidence of bacterial enteritis.
format	article
author	Ling-cong Kong Bo Wang Yi-ming Wang Ren-ge Hu AtiahLujia Atiewin Duo Gao Yun-hang Gao Hong-xia Ma
author_facet	Ling-cong Kong Bo Wang Yi-ming Wang Ren-ge Hu AtiahLujia Atiewin Duo Gao Yun-hang Gao Hong-xia Ma
author_sort	Ling-cong Kong
title	Characterization of bacterial community changes and antibiotic resistance genes in lamb manure of different incidence
title_short	Characterization of bacterial community changes and antibiotic resistance genes in lamb manure of different incidence
title_full	Characterization of bacterial community changes and antibiotic resistance genes in lamb manure of different incidence
title_fullStr	Characterization of bacterial community changes and antibiotic resistance genes in lamb manure of different incidence
title_full_unstemmed	Characterization of bacterial community changes and antibiotic resistance genes in lamb manure of different incidence
title_sort	characterization of bacterial community changes and antibiotic resistance genes in lamb manure of different incidence
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/4f2ea9bb32114f78b7eedc99540c895f
work_keys_str_mv	AT lingcongkong characterizationofbacterialcommunitychangesandantibioticresistancegenesinlambmanureofdifferentincidence AT bowang characterizationofbacterialcommunitychangesandantibioticresistancegenesinlambmanureofdifferentincidence AT yimingwang characterizationofbacterialcommunitychangesandantibioticresistancegenesinlambmanureofdifferentincidence AT rengehu characterizationofbacterialcommunitychangesandantibioticresistancegenesinlambmanureofdifferentincidence AT atiahlujiaatiewin characterizationofbacterialcommunitychangesandantibioticresistancegenesinlambmanureofdifferentincidence AT duogao characterizationofbacterialcommunitychangesandantibioticresistancegenesinlambmanureofdifferentincidence AT yunhanggao characterizationofbacterialcommunitychangesandantibioticresistancegenesinlambmanureofdifferentincidence AT hongxiama characterizationofbacterialcommunitychangesandantibioticresistancegenesinlambmanureofdifferentincidence
_version_	1718387813407260672

Characterization of bacterial community changes and antibiotic resistance genes in lamb manure of different incidence

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