Metabolomic analysis of primary human skeletal muscle cells during myogenic progression

Abstract Skeletal muscle constitutes more than 30% of total body mass using substrates such as glycogen, glucose, free fatty acids, and creatinine phosphate to generate energy. Consequently, multinucleated myofibers and resident mononucleated stem cells (satellite cells) generate several metabolites...

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Autores principales:	Ashok Kumar, Yashwant Kumar, Jayesh Kumar Sevak, Sonu Kumar, Niraj Kumar, Suchitra Devi Gopinath
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4f370ebcd9414a7fa3c9729671712591
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spelling	oai:doaj.org-article:4f370ebcd9414a7fa3c97296717125912021-12-02T15:33:11ZMetabolomic analysis of primary human skeletal muscle cells during myogenic progression10.1038/s41598-020-68796-42045-2322https://doaj.org/article/4f370ebcd9414a7fa3c97296717125912020-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-68796-4https://doaj.org/toc/2045-2322Abstract Skeletal muscle constitutes more than 30% of total body mass using substrates such as glycogen, glucose, free fatty acids, and creatinine phosphate to generate energy. Consequently, multinucleated myofibers and resident mononucleated stem cells (satellite cells) generate several metabolites, which enter into circulation affecting the function of other organs, especially during exercise and atrophy. The present study was aimed at building a comprehensive profile of metabolites in primary human skeletal muscle cells during myogenic progression in an untargeted metabolomics approach using a high resolution Orbitrap Fusion Tribrid Mass Spectrometer. Identification of metabolites with multivariate statistical analyses showed a global shift in metabolomic profiles between myoblasts undergoing proliferation and differentiation along with distinctly separable profiles between early and late differentiating cultures. Pathway analyses of 71 unique metabolites revealed that Pantothenate metabolism and Coenzyme A biosynthesis and Arginine Proline metabolism play dominant roles in proliferating myoblasts, while metabolites involved in vitamin B6, Glyoxylate and Dicarboxylate, Nitrogen, Glutathione, and Tryptophan metabolism were upregulated during differentiation. We found that early and late differentiating cultures displayed differences in Phenylalanine, Tyrosine, Glycine, Serine and Threonine metabolism. Our results identify metabolites during maturation of muscle from progenitor myoblasts that have implications in muscle regeneration and pathophysiology.Ashok KumarYashwant KumarJayesh Kumar SevakSonu KumarNiraj KumarSuchitra Devi GopinathNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ashok Kumar Yashwant Kumar Jayesh Kumar Sevak Sonu Kumar Niraj Kumar Suchitra Devi Gopinath Metabolomic analysis of primary human skeletal muscle cells during myogenic progression
description	Abstract Skeletal muscle constitutes more than 30% of total body mass using substrates such as glycogen, glucose, free fatty acids, and creatinine phosphate to generate energy. Consequently, multinucleated myofibers and resident mononucleated stem cells (satellite cells) generate several metabolites, which enter into circulation affecting the function of other organs, especially during exercise and atrophy. The present study was aimed at building a comprehensive profile of metabolites in primary human skeletal muscle cells during myogenic progression in an untargeted metabolomics approach using a high resolution Orbitrap Fusion Tribrid Mass Spectrometer. Identification of metabolites with multivariate statistical analyses showed a global shift in metabolomic profiles between myoblasts undergoing proliferation and differentiation along with distinctly separable profiles between early and late differentiating cultures. Pathway analyses of 71 unique metabolites revealed that Pantothenate metabolism and Coenzyme A biosynthesis and Arginine Proline metabolism play dominant roles in proliferating myoblasts, while metabolites involved in vitamin B6, Glyoxylate and Dicarboxylate, Nitrogen, Glutathione, and Tryptophan metabolism were upregulated during differentiation. We found that early and late differentiating cultures displayed differences in Phenylalanine, Tyrosine, Glycine, Serine and Threonine metabolism. Our results identify metabolites during maturation of muscle from progenitor myoblasts that have implications in muscle regeneration and pathophysiology.
format	article
author	Ashok Kumar Yashwant Kumar Jayesh Kumar Sevak Sonu Kumar Niraj Kumar Suchitra Devi Gopinath
author_facet	Ashok Kumar Yashwant Kumar Jayesh Kumar Sevak Sonu Kumar Niraj Kumar Suchitra Devi Gopinath
author_sort	Ashok Kumar
title	Metabolomic analysis of primary human skeletal muscle cells during myogenic progression
title_short	Metabolomic analysis of primary human skeletal muscle cells during myogenic progression
title_full	Metabolomic analysis of primary human skeletal muscle cells during myogenic progression
title_fullStr	Metabolomic analysis of primary human skeletal muscle cells during myogenic progression
title_full_unstemmed	Metabolomic analysis of primary human skeletal muscle cells during myogenic progression
title_sort	metabolomic analysis of primary human skeletal muscle cells during myogenic progression
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/4f370ebcd9414a7fa3c9729671712591
work_keys_str_mv	AT ashokkumar metabolomicanalysisofprimaryhumanskeletalmusclecellsduringmyogenicprogression AT yashwantkumar metabolomicanalysisofprimaryhumanskeletalmusclecellsduringmyogenicprogression AT jayeshkumarsevak metabolomicanalysisofprimaryhumanskeletalmusclecellsduringmyogenicprogression AT sonukumar metabolomicanalysisofprimaryhumanskeletalmusclecellsduringmyogenicprogression AT nirajkumar metabolomicanalysisofprimaryhumanskeletalmusclecellsduringmyogenicprogression AT suchitradevigopinath metabolomicanalysisofprimaryhumanskeletalmusclecellsduringmyogenicprogression
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Metabolomic analysis of primary human skeletal muscle cells during myogenic progression

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